DA 3003-2
Based on 1 Customer Validation
DA 3003-2 is a potent and selectively Cdc25 inhibitor. DA 3003-2 shows antiproliferative activity. DA 3003-2 induces cell cycle arrest at the G2/M phase and increases the expression of P-tyr15 Cdc2. DA 3003-2 has the potential for the research of prostate cancer.
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- Purity: 98.36%
- CAS No.: 383907-47-9
- 화학식: C15H16ClN3O3
- 분자량:321.76
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보관:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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Metabolism/Protease Compound Library
HY-L012
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Anti-Cancer Compound Library
HY-L025
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Phosphatase Inhibitor Library
HY-L081
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Anti-Prostate Cancer Compound Library
HY-L124
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Metabolic Enzyme Compound Library
HY-L146
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Highly Selective Inhibitors Library
HY-L158
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CCD-841CoN | IC50 |
>2 μM
Compound: 6b
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Cytotoxicity against human CCD-841CoN cells assessed as growth inhibition after 72 hrs by MTS assay
Cytotoxicity against human CCD-841CoN cells assessed as growth inhibition after 72 hrs by MTS assay
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[PMID: 37302341] |
| DLD-1 | IC50 |
0.59 μM
Compound: 6b
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Cytotoxicity against human DLD1 cells assessed as growth inhibition after 72 hrs by MTS assay
Cytotoxicity against human DLD1 cells assessed as growth inhibition after 72 hrs by MTS assay
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[PMID: 37302341] |
| HEL 299 | IC50 |
>2 μM
Compound: 6b
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Cytotoxicity against human HEL 299 cells assessed as growth inhibition after 72 hrs by MTS assay
Cytotoxicity against human HEL 299 cells assessed as growth inhibition after 72 hrs by MTS assay
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[PMID: 37302341] |
| MDA-MB-231 | IC50 |
0.44 μM
Compound: 6b
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Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTS assay
Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTS assay
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[PMID: 37302341] |
| MRC5 | IC50 |
>2 μM
Compound: 6b
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Cytotoxicity against human MRC5 cells assessed as growth inhibition after 72 hrs by MTS assay
Cytotoxicity against human MRC5 cells assessed as growth inhibition after 72 hrs by MTS assay
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[PMID: 37302341] |
DA 3003-2 (0.3-30 µM; 48 h) shows antiproliferative activity with an IC50 value of 5 µM in PC-3 cells[1].
DA 3003-2 (5, 10 µM; 24, 1 h) induces cell cycle arrest at G2/M phase and increases the expression of P-tyr15 Cdc2 in PC-3 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PC-3 cells
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Concentration:0.3-30 µM
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Incubation Time:48 h
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Result:Showed antiproliferative efficacy in a dose-dependent manner with an IC50 value of 5 µM.
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Cell Line:PC-3 cells
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Concentration:5, 10 µM
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Incubation Time:24 h
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Result:Induced cell cycle arrest at G2/M phase.
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Cell Line:PC-3 cells
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Concentration:5, 10 µM
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Incubation Time:1 h
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Result:Increased the expression of P-tyr15 Cdc2.
Chemical Information
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CAS No. 383907-47-9
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Appearance Solid
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분자량 321.76
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화학식 C15H16ClN3O3
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Color Brown to red
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SMILES
O=C(C1=C(C2=O)N=CC=C1)C(NCCN3CCOCC3)=C2Cl
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Synonyms
NSC663285
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
용액&용해도
DMSO : 150 mg/mL (466.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.75 mg/mL (11.65 mM); Clear solution
This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1079 mL | 15.5395 mL | 31.0791 mL | 77.6977 mL |
| 5 mM | 0.6216 mL | 3.1079 mL | 6.2158 mL | 15.5395 mL | |
| 10 mM | 0.3108 mL | 1.5540 mL | 3.1079 mL | 7.7698 mL | |
| 15 mM | 0.2072 mL | 1.0360 mL | 2.0719 mL | 5.1798 mL | |
| 20 mM | 0.1554 mL | 0.7770 mL | 1.5540 mL | 3.8849 mL | |
| 25 mM | 0.1243 mL | 0.6216 mL | 1.2432 mL | 3.1079 mL | |
| 30 mM | 0.1036 mL | 0.5180 mL | 1.0360 mL | 2.5899 mL | |
| 40 mM | 0.0777 mL | 0.3885 mL | 0.7770 mL | 1.9424 mL | |
| 50 mM | 0.0622 mL | 0.3108 mL | 0.6216 mL | 1.5540 mL | |
| 60 mM | 0.0518 mL | 0.2590 mL | 0.5180 mL | 1.2950 mL | |
| 80 mM | 0.0388 mL | 0.1942 mL | 0.3885 mL | 0.9712 mL | |
| 100 mM | 0.0311 mL | 0.1554 mL | 0.3108 mL | 0.7770 mL |
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