Cat. No.: HY-N6043

Hydroxytyrosol acetate

고객리뷰

Based on 1 Customer Validation

Hydroxytyrosol acetate is an orally active, blood-brain barrier-permeable multi-active compound with multiple effects including antibacterial, antioxidant, anti-platelet aggregation, and neuroprotective activities. Hydroxytyrosol acetate not only inhibits the growth of Vibrio by increasing bacterial membrane permeability, but also interacts with DNA and mediates supercoiled DNA relaxation. Meanwhile, Hydroxytyrosol acetate effectively reduces thrombosis and inhibits lipid oxidation by inhibiting COX activity and promoting vascular nitric oxide production. In terms of neuroprotection, Hydroxytyrosol acetate significantly alleviates neuronal apoptosis and inflammatory responses by up-regulating the expression level of ERβ, thereby improving cognitive function in Alzheimer's disease models. Hydroxytyrosol acetate has been widely used in scientific research related to Vibrio infection, arterial thrombosis, Alzheimer's disease and other related fields.

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Purity: 98.10%
CAS No.: 69039-02-7
화학식: C₁₀H₁₂O₄
분자량:196.20

보관:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Hydroxytyrosol acetate Chemical Structure

CAS No.: 69039-02-7

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
용액&용해도
- In Vitro
- In Vivo
순도&문서
References
Help & FAQs
Bioz

Biological Activity

제품 설명

IC₅₀ & Target

ERβ

All Estrogen Receptor/ERR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	IC₅₀	32 μM Compound: 1	Cytotoxicity against human Caco2/TC7 cells assessed as growth inhibition after 24 hrs by WST-1 assay Cytotoxicity against human Caco2/TC7 cells assessed as growth inhibition after 24 hrs by WST-1 assay	[PMID: 26225717]
HEp-2	CC₅₀	50 μg/mL Compound: 4a	Cytotoxicity against human Hep2 cells after 24 hrs by MTT assay Cytotoxicity against human Hep2 cells after 24 hrs by MTT assay	[PMID: 26260341]
HFF-1	CC₅₀	50 μg/mL Compound: 4a	Cytotoxicity against human HFF1 cells after 24 hrs by MTT assay Cytotoxicity against human HFF1 cells after 24 hrs by MTT assay	[PMID: 26260341]
MRC5	IC₅₀	>50 μM Compound: 10	Cytotoxicity against human MRC5 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 27155468]
Platelet	IC₅₀	10 μM Compound: 1	Inhibition of collagen-induced platelet aggregation in human whole blood preincubated for 10 mins prior to collagen addition measured after 10 mins by aggregometric analysis Inhibition of collagen-induced platelet aggregation in human whole blood preincubated for 10 mins prior to collagen addition measured after 10 mins by aggregometric analysis	[PMID: 26225717]
THP-1	IC₅₀	>10 μM Compound: 10	Antileishmanial activity against Leishmania donovani MHOM/ET/67/HU3 intracellular amastigotes infected in human THP1 cells after 72 hrs by luciferase coupled luminescence assay Antileishmanial activity against Leishmania donovani MHOM/ET/67/HU3 intracellular amastigotes infected in human THP1 cells after 72 hrs by luciferase coupled luminescence assay	[PMID: 27155468]
Vero	CC₅₀	100 μg/mL Compound: 4a	Cytotoxicity against African green monkey Vero cells after 24 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 24 hrs by MTT assay	[PMID: 26260341]

In Vitro

Hydroxytyrosol acetate (39-80 μg/mL; up to 24 h) inhibits growth of Vibrio parahemolyticus with a minimum inhibitory concentration of 39 μg/mL and minimum bactericidal concentration of 78 μg/mL, and 80 μg/mL of the compound almost completely stops bacterial growth during incubation^[1].
Hydroxytyrosol acetate (15-250 min) exhibits time-dependent DPPH radical scavenging activity with EC₅₀ values of 0.26, 0.22, and 0.14 mol AH/mol DPPH at 15, 60, and 250 min, respectively, showing weaker activity than hydroxytyrosol, oleuropein, and 3,4-DHPEA-EA but activity comparable to α-tocopherol^[3].
Hydroxytyrosol acetate (20 μM; 24 h) enhances ERβ promoter activity in HT22 cells, promoting ERβ transcriptional expression^[4].
Hydroxytyrosol acetate (10-20 μM; 24-48 h) enhances ERβ mRNA and protein expression in HT22 cells, with greater effects at higher concentrations or longer incubation times^[4].
Hydroxytyrosol acetate (10 μM; 48 h) protects primary rat hippocampal neurons from Aβ_25-35-induced cell death and apoptosis by restoring ERβ expression and inhibiting apoptotic signaling pathways^[4].
Hydroxytyrosol acetate (10 μM; 48 h) protects primary rat hippocampal neurons from Aβ_25-35-induced apoptosis in an ERβ-dependent manner, as its neuroprotective effects are eliminated when ERβ is knocked down^[4].
Hydroxytyrosol acetate (10 μM; 24 h) alleviates Aβ_25-35-induced synaptic dysfunction in primary rat hippocampal neurons in an in vitro ERβ-dependent manner, as its protective effects on electrophysiological function are eliminated when ERβ is knocked down^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[4]

Cell Line:

HT22 cells
Concentration:

10-20 μM
Incubation Time:

24-48 h
Result:

Enhanced ERβ mRNA and protein expression in a concentrations or times-dependent manner.

In Vivo

Hydroxytyrosol acetate (1-100 mg/kg per day; p.o.; daily; 7 days) administered orally to rats for 7 days inhibits collagen-induced platelet aggregation with an ID₅₀ of 16.05 mg/kg per day, via mechanisms including reduced thromboxane B₂ synthesis and increased nitric oxide production^[2].
Hydroxytyrosol acetate (50 mg/kg; p.o.; once daily; 12 weeks) improves cognitive function, reduces neuronal apoptosis and inflammation, and restores cerebral ERβ expression in female APP/PS1 transgenic Alzheimer's disease mice, without altering Aβ processing^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Wistar Rat (male, 200-250 g)^[2]
Dosage:

1-100 mg/kg per day
Administration:

p.o.; daily; 7 days
Result:

Inhibited collagen-induced platelet aggregation in whole blood in a dose-dependent manner, with an ID₅₀ of 16.05 mg/kg per day.
Inhibited platelet thromboxane B2 production by 37% at 100 mg/kg per day.
Inhibited aortic 6-keto-prostaglandin F1α production by 32% at 100 mg/kg per day.
Increased aortic nitric oxide production by 66% at 100 mg/kg per day compared to control rats.
Did not significantly modify plasma concentrations of 6-keto-prostaglandin F1α.

Animal Model:

APPswe/PS1dE9 (APP/PS1) transgenic mice (female, 12 weeks old at study start, 6.5 months old at testing, 7 months old at sacrifice, on C57BL/6J genetic background)^[4]
Dosage:

50 mg/kg
Administration:

p.o.; once daily; 12 weeks
Result:

Shortened escape latencies in the water maze test on days 2 and 3 of training.
Increased mean number of platform crossings in the probe trial to levels similar to nontransgenic control mice.
Increased spontaneous alternation rate in the T maze test compared to untreated AD mice.
Reduced the relative percentage of TUNEL-positive apoptotic cells in the cerebral cortex and hippocampus to near control levels.
Normalized the increased protein expression of apoptosis markers Bad, Bak, caspase-3, and caspase-9 seen in untreated AD mice.
Reversed elevated serum levels of C-reactive protein (CRP), TNF-α, and IL-1β.
Reversed increased cerebral cortex expression of inflammatory regulators NF-κB, p-p38, p-JNK, and mRNA levels of TNF-α and IL-6.
Restored reduced cerebral cortex levels of p-ERK and p-Akt to control levels.
Restored reduced cerebral cortex ERβ protein and mRNA levels in AD mice to control levels.
Did not affect ERα expression.
Showed no significant effect on soluble Aβ₄₀ or Aβ₄₂ levels, or on expression of APP, BACE, NEP, or IDE compared to untreated AD mice.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 69039-02-7
Appearance Solid
분자량 196.20
화학식 C₁₀H₁₂O₄
Color White to off-white
SMILES

CC(OCCC1=CC(O)=C(O)C=C1)=O
Structure Classification
- Phenols
- Polyphenols
Initial Source
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도

In Vitro:

DMSO : 250 mg/mL (1274.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.0968 mL

25.4842 mL

50.9684 mL

5 mM

1.0194 mL

5.0968 mL

10.1937 mL

10 mM

0.5097 mL

2.5484 mL

5.0968 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 6.25 mg/mL (31.86 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 6.25 mg/mL (31.86 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 6.25 mg/mL (31.86 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wei J, et al. Antibacterial Activity of Hydroxytyrosol Acetate from Olive Leaves (Olea Europaea L.). Nat Prod Res. 2018;32(16):1967-1970. [Content Brief]

[2]. González-Correa JA, et al. Effects of hydroxytyrosol and hydroxytyrosol acetate administration to rats on platelet function compared to acetylsalicylic acid. J Agric Food Chem. 2008;56(17):7872-7876. [Content Brief]

[3]. Gordon MH, et al. Antioxidant activity of hydroxytyrosol acetate compared with that of other olive oil polyphenols. J Agric Food Chem. 2001;49(5):2480-2485. [Content Brief]

[4]. Qin C, et al. Hydroxytyrosol acetate improves the cognitive function of APP/PS1 transgenic mice in ERβ‐dependent manner[J]. Molecular nutrition & food research, 2021, 65(3): 2000797.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.0968 mL	25.4842 mL	50.9684 mL	127.4210 mL
	5 mM	1.0194 mL	5.0968 mL	10.1937 mL	25.4842 mL
	10 mM	0.5097 mL	2.5484 mL	5.0968 mL	12.7421 mL
	15 mM	0.3398 mL	1.6989 mL	3.3979 mL	8.4947 mL
	20 mM	0.2548 mL	1.2742 mL	2.5484 mL	6.3711 mL
	25 mM	0.2039 mL	1.0194 mL	2.0387 mL	5.0968 mL
	30 mM	0.1699 mL	0.8495 mL	1.6989 mL	4.2474 mL
	40 mM	0.1274 mL	0.6371 mL	1.2742 mL	3.1855 mL
	50 mM	0.1019 mL	0.5097 mL	1.0194 mL	2.5484 mL
	60 mM	0.0849 mL	0.4247 mL	0.8495 mL	2.1237 mL
	80 mM	0.0637 mL	0.3186 mL	0.6371 mL	1.5928 mL
	100 mM	0.0510 mL	0.2548 mL	0.5097 mL	1.2742 mL

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Hydroxytyrosol acetate

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All Estrogen Receptor/ERR Isoforms

Biological Activity

Chemical Information

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

Hydroxytyrosol acetate Related Classifications

Cat. No.: HY-N6043