IL-4-IN-2

IL-4-IN-2 (10 μM) potently inhibits the IL-4/IL-13 signaling pathway in HEK Blue IL-4/IL-13 reporter cells, with an inhibition rate of 94.5% at the concentration of 10 μM. Its EC₅₀ values for IL-4- and IL-13-induced signaling are 627.2 nM and 18.25 μM, respectively^[1].
IL-4-IN-2 (2 μM) binds to soluble IL-4, resulting in a thermal stability change (ΔT_m) of 1.66°C, while no thermal stability change meeting the binding criteria is observed for IL-2, IL-7, IL-13, and IL-21^[1].
IL-4-IN-2 (50-5000 nM) dose-dependently inhibits IL-4-induced STAT-6 phosphorylation in THP-1 monocytes at concentrations of 50 nM, 500 nM, and 5 μM^[1].
IL-4-IN-2 (50-5000 nM) inhibits IL-4-induced STAT-6 phosphorylation in Ramos B lymphocytes at concentrations of 50 nM, 500 nM, and 5 μM^[1].
IL-4-IN-2 (up to 25 μM; 24 h) shows no significant cytotoxicity in B16-F10 cells^[1].
IL-4-IN-2 shows a retention rate of 75.4% after 1 h of incubation in mouse liver microsomes, and its retention rates are both over 99% after 3 h of incubation in human and mouse plasma, with a PAMPA membrane permeability coefficient of 6.2 × 10^-6 cm/s

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.