NBD-556
Based on 1 Customer Validation
NBD-556, a CD4 mimetic, is a potent HIV-1 entry inhibitor that blocks the gp120-CD4 interaction. NBD-556 shows potent cell fusion and virus-cell fusion inhibitory activity at low micromolar levels.
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- Purity: 99.57%
- CAS No.: 333353-44-9
- 화학식: C17H24ClN3O2
- 분자량:337.84
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
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HIV-1 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MT2 | CC50 |
>280 μM
Compound: 1; NBD-556
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Cytotoxicity against human MT2 cells assessed as reduction in cell viability incubated for 4 days by XTT assay
Cytotoxicity against human MT2 cells assessed as reduction in cell viability incubated for 4 days by XTT assay
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[PMID: 34992041] |
| MT2 | CC50 |
150 μM
Compound: 1, NBD-556
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Cytotoxicity against human MT2 cells after 4 days by XTT assay
Cytotoxicity against human MT2 cells after 4 days by XTT assay
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[PMID: 22524483] |
| MT2 | IC50 |
2.1 μM
Compound: 1
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Antiviral activity against HIV1 3B infected in human MT2 cells assessed as protection against virus-induced cytopathogenicity by MTT assay
Antiviral activity against HIV1 3B infected in human MT2 cells assessed as protection against virus-induced cytopathogenicity by MTT assay
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[PMID: 20728351] |
| MT2 | IC50 |
8 μM
Compound: 1, NBD-556
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Inhibition of X4-tropic HIV1 3B gp120-mediated entry into human MT2 cells assessed as Inhibition of p24 production incubated overnight followed by compound wash-out measured after 4 days post-infection by multi-cycle neutralization assay
Inhibition of X4-tropic HIV1 3B gp120-mediated entry into human MT2 cells assessed as Inhibition of p24 production incubated overnight followed by compound wash-out measured after 4 days post-infection by multi-cycle neutralization assay
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[PMID: 22524483] |
| TZM | CC50 |
132 μM
Compound: NBD-556
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Cytotoxicity against human TZM-bl cells assessed as reduction in cell viability by Cell Titer-Glo assay
Cytotoxicity against human TZM-bl cells assessed as reduction in cell viability by Cell Titer-Glo assay
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[PMID: 33497209] |
| TZM | CC50 |
177 μM
Compound: NBD-556; 1
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Cytotoxicity against human TZM-bl cells assessed as reduction in cell viability by CellTiter-Glo 2.0 assay
Cytotoxicity against human TZM-bl cells assessed as reduction in cell viability by CellTiter-Glo 2.0 assay
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[PMID: 35063895] |
| TZM | CC50 |
60 μM
Compound: 1; NBD-556
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Cytotoxicity against human TZM-bl cells assessed as reduction in cell viability incubated for 3 days by XTT assay
Cytotoxicity against human TZM-bl cells assessed as reduction in cell viability incubated for 3 days by XTT assay
|
[PMID: 34992041] |
| TZM | CC50 |
60 μM
Compound: NBD-556
|
Cytotoxicity against human TZM-bl cells assessed as reduction in cell viability incubated for 3 days by XTT assay
Cytotoxicity against human TZM-bl cells assessed as reduction in cell viability incubated for 3 days by XTT assay
|
[PMID: 34246921] |
NBD-556 (1-100 μM) inhibits HIV-1 envelope-mediated virus-cell and cell-cell fusion[1].
NBD-556 inhibits the infection of MT-2 cells by laboratory-adapted HIV-1 IIIB, MN, and V32 strains, with IC50s of 6.5, 15.9, and 5.3 μM, respectively[1].
NBD-556 (1-100 μM) inhibits the CD4-dependent virus in a dose-dependent manner with an IC50 of 22.6 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 333353-44-9
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Appearance Solid
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분자량 337.84
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화학식 C17H24ClN3O2
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Color White to off-white
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SMILES
O=C(C(NC1CC(C)(NC(C)(C1)C)C)=O)NC2=CC=C(C=C2)Cl
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
용액&용해도
DMSO : 33.33 mg/mL (98.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (271 KB)
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SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Portuguese - PT (420 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhao Q, Ma L, Jiang S, Lu H, Liu S, He Y, Strick N, Neamati N, Debnath AK. Identification of N-phenyl-N'-(2,2,6,6-tetramethyl-piperidin-4-yl)-oxalamides as a new class of HIV-1 entry inhibitors that prevent gp120 binding to CD4. Virology. 2005 Sep 1;339(2):213-25. [Content Brief]
[2]. Schon A, et al. Thermodynamics of binding of a low-molecular-weight CD4 mimetic to HIV-1 gp120. Biochemistry. 2006 Sep 12;45(36):10973-10980. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9600 mL | 14.7999 mL | 29.5998 mL | 73.9995 mL |
| 5 mM | 0.5920 mL | 2.9600 mL | 5.9200 mL | 14.7999 mL | |
| 10 mM | 0.2960 mL | 1.4800 mL | 2.9600 mL | 7.4000 mL | |
| 15 mM | 0.1973 mL | 0.9867 mL | 1.9733 mL | 4.9333 mL | |
| 20 mM | 0.1480 mL | 0.7400 mL | 1.4800 mL | 3.7000 mL | |
| 25 mM | 0.1184 mL | 0.5920 mL | 1.1840 mL | 2.9600 mL | |
| 30 mM | 0.0987 mL | 0.4933 mL | 0.9867 mL | 2.4667 mL | |
| 40 mM | 0.0740 mL | 0.3700 mL | 0.7400 mL | 1.8500 mL | |
| 50 mM | 0.0592 mL | 0.2960 mL | 0.5920 mL | 1.4800 mL | |
| 60 mM | 0.0493 mL | 0.2467 mL | 0.4933 mL | 1.2333 mL | |
| 80 mM | 0.0370 mL | 0.1850 mL | 0.3700 mL | 0.9250 mL |
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