Rat Factor Xa
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Rat Factor Xa is a serine protease that plays a central role in blood coagulation, with prothrombin as its physiological substrate. Rat Factor Xa catalyzes the conversion of prothrombin to thrombin, activates factor VIII, and hydrolyzes peptide, thioester, and amide substrates. Rat Factor Xa cleaves protease-activated receptors 1 and 2 to activate intracellular signal transduction. Rat Factor Xa regulates multiple cellular pathways, mediates various cellular activities, tissue remodeling and cancer cell migration, and inhibits apoptosis in some cancer cells. Rat Factor Xa protects hippocampal neurons against glutamate-induced damage. Rat Factor Xa participates in embryonic vascular development and regulates immune hematopoiesis; its activity is inhibited by soybean trypsin inhibitor, and cannot be enhanced by phospholipids. Rat Factor Xa regulates immune responses and macrophage polarization, and participates in fibrosis, vascular remodeling and tumor progression through non-coagulant effects. Rat Factor Xa can be used in research related to atherosclerosis, fibrosis, asthma, cancer, thromboinflammation, sepsis, etc.
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- CAS No.: 9002-05-5
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- 순도&문서
- References
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Help & FAQs
Biological Activity
Rat Factor Xa (15-250 μM) and its Gla-domain truncated form (Factor Xa(-GD)) exhibit indistinguishable peptide p-nitroanilide hydrolytic activity, with nearly identical Km and kcat values for both tested substrates[2].
Rat Factor Xa (0.16 μM; up to 60 minutes) and its Gla-domain truncated form (Factor Xa(-GD)) exhibit indistinguishable prothrombin activation activity in solution (with or without Factor Va), but only native Rat Factor Xa shows enhanced activity in the presence of phospholipid[2].
Rat Factor Xa retains full one-stage clotting activity when stored at 4 °C for 4.5 months in the presence of 5 mM CaCl2, whereas activity drops to 20% of initial levels in the absence of CaCl2[2].
Rat Factor Xa has chymotryptic proteolysis inhibited by divalent cations (Ca2+, Mg2+), with Ca2+ providing near-complete inhibition at concentrations >2 mM and a half-maximal inhibitory concentration of 0.6 mM[2].
Rat Factor Xa (5.5-55 nM) hydrolyzes a range of arginine-containing thioester substrates with highest specificity (kcat/Km up to 1.6 × 105 M-1s-1) for substrates with a P2 Gly residue, consistent with its natural prothrombin substrate[4].
Rat Factor Xa hydrolyzes Z-Trp-Arg-NA and Z-Trp-Arg-AMC amide substrates with kcat/Km values around 1.0 × 104 M-1s-1, exhibiting unusual reactivity with amides compared to other coagulation factors[4].
Rat Factor Xa (5.5-55 nM) hydrolyzes select P'-extended thioester substrates with reduced reactivity (kcat/Km up to 4.5 × 104 M-1s-1) relative to simple thioester substrates, showing a slight preference for the P1' S-Ile analogue over S-Val[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 9002-05-5
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SMILES
[Rat Factor Xa]
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
순도&문서
References
[1]. Borensztajn K, et al. Factor Xa: at the crossroads between coagulation and signaling in physiology and disease. Trends in molecular medicine. 2008 Oct;14(10):429-40. [Content Brief]
[2]. Morita T, et al. Preparation and properties of derivatives of bovine factor X and factor Xa from which the gamma-carboxyglutamic acid containing domain has been removed. The Journal of biological chemistry. 1986 Mar 25;261(9):4015-23. [Content Brief]
[3]. Stenn KS, et al. Mechanism of bovine prothrombin activation by an insoluble preparation of bovine factor Xa (thrombokinase). Biochemistry. 1972 Nov 21;11(24):4502-15. [Content Brief]
[4]. McRae BJ, et al. Mapping the active sites of bovine thrombin, factor IXa, factor Xa, factor XIa, factor XIIa, plasma kallikrein, and trypsin with amino acid and peptide thioesters: development of new sensitive substrates. Biochemistry. 1981 Dec 08;20(25):7196-206. [Content Brief]
[5]. Ruf W, et al. Roles of factor Xa beyond coagulation. Journal of thrombosis and thrombolysis. 2021 Aug;52(2):391-396. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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