USP10-IN-3
Based on 1 Customer Validation
USP10-IN-3 (compound D1) is a potent USP10 inhibitor with an IC50 value of 7.2 µM. USP10-IN-3 inhibits cell proliferation. USP10-IN-3 induces apoptosis and cell cycle arrest at S-phase.
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- Purity : 98.00%
- CAS No.: 3059565-11-3
- 화학식: C29H25ClN4O3
- 분자량:512.99
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보관:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
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USP10 7.2 μM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Bel-7402 | IC50 |
2.3 μM
Compound: D1
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Antiproliferative activity against human Bel-7402 cells by CCK-8 method
Antiproliferative activity against human Bel-7402 cells by CCK-8 method
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[PMID: 38718626] |
In Vitro
USP10-IN-3 (compound D1) (0-100 µM) inhibits cell proliferation with an IC50 value of 2.3 µM for Huh-7 cells[1].
USP10-IN-3 (compound D1) (0-10 µM; 24 h) induces apoptosis and cell cycle arrest at S-phase in a dose-dependent manner[1].
USP10-IN-3 (compound D1) (0-30 µM; 24 h) decreases the expression of YAP1, p53, p21 in a dose-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Huh-7 cells
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Concentration:0, 0.625, 1.25, 2.5, 5, 10 µM
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Incubation Time:24 h
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Result:Induced apoptosis in a dose-dependent manner.
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Cell Line:Huh-7 cells
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Concentration:0, 0.625, 1.25, 2.5, 5 µM
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Incubation Time:24 h
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Result:Induced cell cycle arrest at S-phase.
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Cell Line:Huh-7 cells
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Concentration:0, 0.37, 1.1, 3.3, 10, 30 µM
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Incubation Time:24 h
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Result:Decreased the expression of YAP1, p53, p21 in a dose-dependent manner.
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- PCNA Immunodetection Proliferation Assay
- Flow cytometric DNA-content cell-cycle staining
- Protocol for Cell Counting and Cell Density Analysis
- Apoptosis Solutions
- MTT Cell Proliferation Assay
Chemical Information
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CAS No. 3059565-11-3
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Appearance Solid
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분자량 512.99
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화학식 C29H25ClN4O3
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Color White to off-white
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SMILES
CC1=CC=C2C(C(C3=NC(C4=C(C=CC=C4)C)=NO3)=CN(C2=C1)CC(NCCC5=CC=C(C=C5)Cl)=O)=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
In Vitro:
DMSO : 5 mg/mL (9.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (278 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9494 mL | 9.7468 mL | 19.4936 mL | 48.7339 mL |
| 5 mM | 0.3899 mL | 1.9494 mL | 3.8987 mL | 9.7468 mL |
USP10-IN-3 Related Classifications
HY-163622 Related Classifications
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