2. Neuronal Signaling
  3. Cholinesterase (ChE)
  4. Methylene Violet 3RAX

Methylene Violet 3RAX is a phenazine dye to stain the mitochondria of cells. Methylene Violet 3RAX can change the molecular structure of DNA, undermine the module of DNA, and induce the generation of the reactive singlet oxygen. Methylene Violet 3RAX shows inhibition for human erythrocyte AChE and human plasma BChE with an Kis of 1.58, 0.51 μM, respectively. Methylene Violet 3RAX has the potential for the research of potential photosensitizers for mitochondrial targeting action in PDT (photodynamic therapy).

For research use only. We do not sell to patients.

Methylene Violet 3RAX

Methylene Violet 3RAX Chemical Structure

CAS No. : 4569-86-2

Size Price Stock Quantity
Powder + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Powder
500 mg In-stock
1 g In-stock
5 g In-stock
10 g In-stock
25 g In-stock
50 g   Get quote  

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Based on 1 publication(s) in Google Scholar

Methylene Violet 3RAX Related Antibodies

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Description

Methylene Violet 3RAX is a phenazine dye to stain the mitochondria of cells. Methylene Violet 3RAX can change the molecular structure of DNA, undermine the module of DNA, and induce the generation of the reactive singlet oxygen. Methylene Violet 3RAX shows inhibition for human erythrocyte AChE and human plasma BChE with an Kis of 1.58, 0.51 μM, respectively. Methylene Violet 3RAX has the potential for the research of potential photosensitizers for mitochondrial targeting action in PDT (photodynamic therapy)[1][2].

IC50 & Target[2]

BChE

0.51 μM (Ki)

AChE

1.58 μM (Ki)

In Vitro

Methylene Violet 3RAX shows antitumor activity by cutting off DNA strands in tumour cell[1]. Methylene Violet 3RAX (0-400 µM) significantly enhances cleavage of DNA with over 60% photocleavage activity towards DNA at 300 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Methylene Violet 3RAX Related Antibodies
Molecular Weight

378.90

Formula

C22H23ClN4
CAS No.
Appearance

Powder

Color

Green to dark green

SMILES

[Cl-].N=1C=2C=CC(N)=CC2[N+](C=3C=CC=CC3)=C4C=C(C=CC14)N(CC)CC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (65.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6392 mL 13.1961 mL 26.3922 mL
5 mM 0.5278 mL 2.6392 mL 5.2784 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 95.54%

SDS (394 KB)

COA (250 KB) HNMR (115 KB) RP-HPLC (246 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6392 mL 13.1961 mL 26.3922 mL 65.9805 mL
5 mM 0.5278 mL 2.6392 mL 5.2784 mL 13.1961 mL
10 mM 0.2639 mL 1.3196 mL 2.6392 mL 6.5980 mL
15 mM 0.1759 mL 0.8797 mL 1.7595 mL 4.3987 mL
20 mM 0.1320 mL 0.6598 mL 1.3196 mL 3.2990 mL
25 mM 0.1056 mL 0.5278 mL 1.0557 mL 2.6392 mL
30 mM 0.0880 mL 0.4399 mL 0.8797 mL 2.1993 mL
40 mM 0.0660 mL 0.3299 mL 0.6598 mL 1.6495 mL
50 mM 0.0528 mL 0.2639 mL 0.5278 mL 1.3196 mL
60 mM 0.0440 mL 0.2199 mL 0.4399 mL 1.0997 mL
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Methylene Violet 3RAX Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methylene Violet 3RAX4569-86-2Cholinesterase (ChE)photodynamic therapyantitumorphotosensitizerscleavage of DNAInhibitorinhibitorinhibit

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