2. Epigenetics Apoptosis
  3. METTL3 c-Myc Bcl-2 Family
  4. METTL3-IN-11

METTL3-IN-11 is an excellent, selective METTL3 inhibitor (IC50 = 45.31 nM). METTL3-IN-11 exhibits high selectivity towards METTL3 compared to DNMT1, EZH1, MLL1, and PRMT1. METTL3-IN-11 reduces the m6A level of total RNA in MOLM-13 and SKOV3 cells, induces cell apoptosis, and inhibits cell migration. METTL3-IN-11 can reduce the expression of m6A downstream target genes (c-MYC and BCL2). METTL3-IN-11 can be used for the study of ovarian cancer and acute myeloid leukemia.

For research use only. We do not sell to patients.

METTL3-IN-11

METTL3-IN-11 Chemical Structure

CAS No. : 3069369-05-4

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METTL3-IN-11 Related Antibodies

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Description

METTL3-IN-11 is an excellent, selective METTL3 inhibitor (IC50 = 45.31 nM). METTL3-IN-11 exhibits high selectivity towards METTL3 compared to DNMT1, EZH1, MLL1, and PRMT1. METTL3-IN-11 reduces the m6A level of total RNA in MOLM-13 and SKOV3 cells, induces cell apoptosis, and inhibits cell migration. METTL3-IN-11 can reduce the expression of m6A downstream target genes (c-MYC and BCL2). METTL3-IN-11 can be used for the study of ovarian cancer and acute myeloid leukemia[1].

IC50 & Target

Bcl-2

 

In Vitro

METTL3-IN-11 (Compound 30t) exhibits good antiproliferative activity in both SKOV3 (IC50 = 6.42 μM) and MOLM-13 (IC50 = 12.34 μM) cells, while showing low toxicity in LO2 and HK2 cells, with the survival rate of HK2 cells at 25 μM and 50 μM being significantly higher than that of the positive control STM2457[1].
METTL3-IN-11 (5-15 μM) reduces the m6A levels on total RNA in a dose-dependent manner in SKOV3 and MOLM-13 cells[1].
METTL3-IN-11 (10-25 μM, 48 h) significantly inhibits the migratory capability of SKOV3 cells and induces apoptosis[1].
METTL3-IN-11 (10-25 μM, 48 h) does not affect the expression level of METTL3 protein, but it downregulates the protein expression of downstream target genes of m6A (such as c-MYC and BCL2) in SKOV3 and MOLM-13 cells in a dose-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

METTL3-IN-11 Related Antibodies

Cell Migration Assay [1]

Cell Line: SKOV3 cells
Concentration: 10 μM, 25 μM
Incubation Time: 48 h
Result: Significantly inhibits the migratory capability of SKOV3 cells.

Western Blot Analysis[1]

Cell Line: SKOV3 cells, MOLM-13 cells
Concentration: 10 μM, 25 μM
Incubation Time: 48 h
Result: Did not affect the expression level of METTL3 protein.
Downregulated the protein expression of downstream target genes of m6A (such as c-MYC and BCL2).
Parmacokinetics
Species Dose Route T1/2 MRT AUC0-∞ AUC0-t Cmax Tmax F CL/F
Rat 1 mg/kg i.v. 9.92 h 8.96 h 204.4 ng·h/mL 176.07 ng·h/mL 168.27 ng/mL 0.083 h / /
Rat 3 mg/kg p.o. 5.88 h 4.71 h 106.96 ng·h/mL 102.55 ng·h/mL 40.84 ng/mL 2 h 17.44 % 0.028086 h
In Vivo

METTL3-IN-11 (Compound 30t) (50 mg/kg, i.p., once daily for 2 weeks) significantly inhibits tumor growth in mice bearing SKOV3 cells and maintains stable body weight during the experiment, and no obvious toxic reactions are observed[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: SKOV3 cells were subcutaneously inoculated into the right flank of female BALB/c mice (4–5 weeks old)[1].
Dosage: 50 mg/kg
Administration: I.p., once daily for 2 weeks
Result: Significantly inhibited tumor growth.
No significant difference was observed in the body weight.
Molecular Weight

472.54

Formula

C26H28N6O3
CAS No.
SMILES

OC1=C(CN2C=NC(C(NCC3=CN4C(C=CC(CNCC5CCC5)=C4)=N3)=O)=CC2=O)C=CC=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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METTL3-IN-11 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

METTL3-IN-113069369-05-4METTL3c-MycBcl-2 FamilyMethyltransferase-like 3MycAcute myeloid leukemiaImidazo[1,2-a]pyridineMETTL3 inhibitorOvarian carcinomaInhibitorinhibitorinhibit

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