MptpB-IN-3 

MptpB-IN-3 is a selective inhibitor of Mycobacterium tuberculosis protein tyrosine phosphatase B (MptpB) with an IC₅₀ of 0.19 μM. MptpB-IN-3 blocks MptpB-mediated inhibition of the macrophage MAPK pathway and restores the phosphorylation levels of Erk1/2 and p38. MptpB-IN-3 exhibits direct anti-tuberculosis activity against Mycobacterium tuberculosis and reduces the Mycobacterium tuberculosis load in mouse macrophages. MptpB-IN-3 can be used for tuberculosis research^[1].

MptpB-IN-3 (Compound 4) inhibits purified recombinant human PTP1B with an IC₅₀ of 65.61 μM, demonstrating 345-fold selectivity for Mycobacterium tuberculosis MptpB over the human phosphatase^[1].
MptpB-IN-3 inhibits the growth of drug-sensitive Mycobacterium tuberculosis H37Rv with a MIC of 1.94 μg/mL^[1].
MptpB-IN-3 inhibits the growth of multi-drug-resistant Mycobacterium tuberculosis strains 13946 and 14862 with MICs of 3.80 μg/mL and 4.60 μg/mL, respectively, but is inactive against a pretomanid-resistant strain (MIC >32 μg/mL)^[1].
MptpB-IN-3 (0.26-64 μg/mL; 48 h) exhibits no cytotoxicity against Vero and J774A.1 macrophage cells (IC₅₀ >64 μg/mL) and low cytotoxicity against HepG2 cells (IC₅₀ = 55.92 μg/mL)^[1].
MptpB-IN-3 (1-10 μg/mL) reduces Mycobacterium tuberculosis burden in infected J774A.1 macrophages in a dose-dependent manner, and exhibits greater potency than Rifampicin and PA-824 at equivalent doses^[1].
MptpB-IN-3 (5-20 μM; 3 h) specifically restores Erk1/2 and p38 phosphorylation in IFN-γ-stimulated Raw264.7 cells overexpressing wild-type Mycobacterium tuberculosis MptpB, reversing MptpB-mediated suppression of the MAPK pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MptpB-IN-3 (Compound 4) (5-50 mg/kg; p.o., i.v.; single dose) exhibits acceptable pharmacokinetic properties in male ICR mice, with an oral bioavailability of 4.9%^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

536.55

C₂₂H₂₁FN₄O₇S₂

3104354-58-4

CCCC[C@H](N1C(/C(SC1=S)=C/C2=CC(F)=C(C=C2)O[C@@H]3COC4=NC([N+]([O-])=O)=CN4C3)=O)C(O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Shao H, et al. Rhodanine-Inspired Development of Dual-Action Mycobacterium tuberculosis Protein Tyrosine Phosphatase B Inhibitors. J Med Chem. 2026 May 6.