MRT-83 hydrochloride
Based on 1 Customer Validation
MRT-83 (hydrochloride) is the potent antagonist of Smoothened (Smo) receptor. MRT-83 (hydrochloride) inhibits the Hedgehog (Hh) signaling pathway and BODIPY-cyclopamine binding to human Smo. MRT-83 (hydrochloride) has the potential for researching cancer disease.
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- Pureté : 99.60%
- CAS No.: 1359944-60-7
- Formule: C31H31ClN4O5
- Masse moléculaire:575.05
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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HY-12848
SAGSmoothened Agonist -
HY-12848B
SAG hydrochlorideSmoothened Agonist -
HY-12848R
SAG (Standard)Smoothened Agonist -
HY-16587
SaridegibSmo Inhibitor -
HY-100224
SANT-1Smo Antagonist -
HY-15108
PurmorphamineSmoothened Receptor Agonist -
HY-16582
Sonidegib diphosphateSmo Antagonist -
HY-16391
Glasdegib -
HY-16582A
SonidegibSmo Antagonist -
HY-13459
PF-5274857Smo Antagonist -
HY-15108G
Purmorphamine (GMP)Smo Receptor Agonist (GMP) -
HY-12316
20(S)-HydroxycholesterolSmo Activator
Activité biologique
Description
IC50 & Target
Smo[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HT-1080 | IC50 |
29 μM
Compound: 4; ST8117
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Antiproliferative activity against human HT1080 cells after 72 hrs by sulforhodamine-B assay
Antiproliferative activity against human HT1080 cells after 72 hrs by sulforhodamine-B assay
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[PMID: 30099257] |
| LS180 | IC50 |
9.7 μM
Compound: 4; ST8117
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Antiproliferative activity against human LS180 cells after 72 hrs by sulforhodamine-B assay
Antiproliferative activity against human LS180 cells after 72 hrs by sulforhodamine-B assay
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[PMID: 30099257] |
In Vitro
MRT-83 (hydrochloride) (compound 86) has IC50s of 15 and 11 nM respectively in Shh-light2 and C3H10T1/2 cells in the biological activity assay[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1359944-60-7
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Appearance Solid
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Masse moléculaire 575.05
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Formule C31H31ClN4O5
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Color White to off-white
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SMILES
O=C(C1=CC=C(C2=CC=CC=C2)C=C1)NC3=CC(NC(NC(C4=CC(OC)=C(OC)C(OC)=C4)=O)=N)=CC=C3C.Cl
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
In Vitro:
DMSO : 250 mg/mL (434.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.62 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.62 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7390 mL | 8.6949 mL | 17.3898 mL | 43.4745 mL |
| 5 mM | 0.3478 mL | 1.7390 mL | 3.4780 mL | 8.6949 mL | |
| 10 mM | 0.1739 mL | 0.8695 mL | 1.7390 mL | 4.3474 mL | |
| 15 mM | 0.1159 mL | 0.5797 mL | 1.1593 mL | 2.8983 mL | |
| 20 mM | 0.0869 mL | 0.4347 mL | 0.8695 mL | 2.1737 mL | |
| 25 mM | 0.0696 mL | 0.3478 mL | 0.6956 mL | 1.7390 mL | |
| 30 mM | 0.0580 mL | 0.2898 mL | 0.5797 mL | 1.4491 mL | |
| 40 mM | 0.0435 mL | 0.2174 mL | 0.4347 mL | 1.0869 mL | |
| 50 mM | 0.0348 mL | 0.1739 mL | 0.3478 mL | 0.8695 mL | |
| 60 mM | 0.0290 mL | 0.1449 mL | 0.2898 mL | 0.7246 mL | |
| 80 mM | 0.0217 mL | 0.1087 mL | 0.2174 mL | 0.5434 mL | |
| 100 mM | 0.0174 mL | 0.0869 mL | 0.1739 mL | 0.4347 mL |
MRT-83 hydrochloride Related Classifications
HY-18287A Related Classifications
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