Cat. No.: HY-149636

Multi-target kinase inhibitor 2

Customer Review

Based on 1 Customer Validation

Multi-target kinase inhibitor 2 is a multi-targeted kinase inhibitor, and exhibits activity against EGFR, Her2, VEGFR2, and CDK2 enzymes, with IC₅₀ values of 79 nM, 40 nM,136 nM, and 204 nM, respectively. Multi-target kinase inhibitor 2 shows cytotoxic effects were observed against HepG2, HeLa , MDA-MB-231 and MCF-7, with IC₅₀ of 41, 57, 51 and 59 μM. Multi-target kinase inhibitor 2 induces cell cycle arrest and apoptosis in HepG2 cells.

For research use only. We do not sell to patients.

Purity: 99.82%
Formula: C₂₀H₁₄Cl₂N₆O
Molecular Weight:425.27

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Multi-target kinase inhibitor 2 Chemical Structure

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Price

Stock

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Amount: USD 0.00

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Top Publications Citing

Use of MCE Products

Antibody-Drug Conjugates

One-stop solution for XDC (ADC/PDC/RDC/ApDC/FDC/ISAC/VDC/SMDC)

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

HER2

40 nM (IC₅₀)

EGFR

79 nM (IC₅₀)

VEGFR2

136 nM (IC₅₀)

CDK2

204 nM (IC₅₀)

All EGFR Isoforms :

All VEGFR Isoforms :

All CDK Isoforms :

In Vitro

Multi-target kinase inhibitor 2 (compound 5K) (41 μM; 24 h) induces cell cycle arrest and apoptosis in HepG2 cells, accompanied by a notable increase in proapoptotic proteins caspase-3 and Bax, as well as the downregulation of Bcl-2 activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:

HepG2 cells
Concentration:

41 μM
Incubation Time:

24 h
Result:

Increased in the G1 phase and decreased in the S and G2/M phases.

Apoptosis Analysis^[1]

Cell Line:

HepG2 cells
Concentration:

41 μM
Incubation Time:

24 h
Result:

Induced apoptosis in HepG2 cells, particularly in the early stages.

Western Blot Analysis^[1]

Cell Line:

HepG2 cells
Concentration:

41 μM
Incubation Time:

24 h
Result:

Increased the levels of caspase-3 and Bax.
The level of the anti-apoptotic protein Bcl-2 was lower.

Chemical Information

Appearance Solid
Molecular Weight 425.27
Formula C₂₀H₁₄Cl₂N₆O
Color White to off-white
SMILES

O=C(N/N=C/C1=CC(Cl)=C(Cl)C=C1)C2=CC=C(C=C2)NC3=NC=NC4=C3C=CN4
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 20 mg/mL (47.03 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3514 mL

11.7572 mL

23.5145 mL

5 mM

0.4703 mL

2.3514 mL

4.7029 mL

10 mM

0.2351 mL

1.1757 mL

2.3514 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Alotaibi AA, et al. Discovery of New Pyrrolo[2,3-d]pyrimidine Derivatives as Potential Multi-Targeted Kinase Inhibitors and Apoptosis Inducers. Pharmaceuticals (Basel). 2023;16(9):1324. Published 2023 Sep 19. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3514 mL	11.7572 mL	23.5145 mL	58.7862 mL
	5 mM	0.4703 mL	2.3514 mL	4.7029 mL	11.7572 mL
	10 mM	0.2351 mL	1.1757 mL	2.3514 mL	5.8786 mL
	15 mM	0.1568 mL	0.7838 mL	1.5676 mL	3.9191 mL
	20 mM	0.1176 mL	0.5879 mL	1.1757 mL	2.9393 mL
	25 mM	0.0941 mL	0.4703 mL	0.9406 mL	2.3514 mL
	30 mM	0.0784 mL	0.3919 mL	0.7838 mL	1.9595 mL
	40 mM	0.0588 mL	0.2939 mL	0.5879 mL	1.4697 mL

Keywords

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Multi-target kinase inhibitor 2

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Protein Tyrosine Kinase Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Reprogramming Compound Library

Anti-Hepatitis C Virus Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Cytokine Inhibitors Library

Cell Death Library

Serine/Threonine Kinase Inhibitor Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

MCE Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

High-Efficiency Gene Editing Compound Library

All EGFR Isoforms

All VEGFR Isoforms

All CDK Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Multi-target kinase inhibitor 2 Related Classifications

HY-149636 Related Classifications