NADs-IN-2
Based on 1 Customer Validation
NADs-IN-2 is a NADSYN1 inhibitor. NADs-IN-2 also binds to the NaAD subsite within the active site of bacterial NADs, thereby inhibiting enzymatic function. NADs-IN-2 can be used in research related to anthrax and cancer.
For research use only. We do not sell to patients.
- Purity : 95.63%
- CAS No.: 706772-75-0
- Formula: C19H14Cl2N4O5S
- Molecular Weight:481.31
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
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NADSYN1 |
In Vitro
NADs-IN-2 (2 μM; 72 h) fails to induce drug sensitivity in non-cancer cells (IMR90, RPE-1) with NAPRT- or NAMPT-overexpression[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:5-6 week-old female athymic nu/nu mice (nude mice)[1]
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Dosage:10 mg/kg; 30mg/kg
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Administration:i.p.; once daily; for two weeks
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Result:Reduced the volume and intratumoral NAD+ levels of Preiss Handler pathway-dependent OV4PH-amp tumors in nude mice.
Had no significant effect on salvage pathway-dependent H460Sal-dep tumors.
Chemical Information
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CAS No. 706772-75-0
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Appearance Solid
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Molecular Weight 481.31
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Formula C19H14Cl2N4O5S
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Color Light yellow to yellow
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SMILES
O=C(NC1=CC=C(S(=O)(NC2=CC=C(C(Cl)=C2)Cl)=O)C=C1)NC3=CC=C([N+]([O-])=O)C=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (207.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.19 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.0777 mL | 10.3883 mL | 20.7766 mL | 51.9416 mL |
| 5 mM | 0.4155 mL | 2.0777 mL | 4.1553 mL | 10.3883 mL | |
| 10 mM | 0.2078 mL | 1.0388 mL | 2.0777 mL | 5.1942 mL | |
| 15 mM | 0.1385 mL | 0.6926 mL | 1.3851 mL | 3.4628 mL | |
| 20 mM | 0.1039 mL | 0.5194 mL | 1.0388 mL | 2.5971 mL | |
| 25 mM | 0.0831 mL | 0.4155 mL | 0.8311 mL | 2.0777 mL | |
| 30 mM | 0.0693 mL | 0.3463 mL | 0.6926 mL | 1.7314 mL | |
| 40 mM | 0.0519 mL | 0.2597 mL | 0.5194 mL | 1.2985 mL | |
| 50 mM | 0.0416 mL | 0.2078 mL | 0.4155 mL | 1.0388 mL | |
| 60 mM | 0.0346 mL | 0.1731 mL | 0.3463 mL | 0.8657 mL | |
| 80 mM | 0.0260 mL | 0.1299 mL | 0.2597 mL | 0.6493 mL | |
| 100 mM | 0.0208 mL | 0.1039 mL | 0.2078 mL | 0.5194 mL |
NADs-IN-2 Related Classifications
HY-189475 Related Classifications
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