2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. Calcium Channel
  4. Ned 19

Ned 19 is a selective membrane-permeant non competitive NAADP antagonist and inhibits NAADP-mediated Ca2+ signaling, with an IC50 of 65 nM. Ned 19 strongly inhibits tumor growth and vascularization as well as lung metastases in mice.

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Ned 19 Chemical Structure

Ned 19 Chemical Structure

CAS No. : 874374-25-1

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 192 In-stock
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10 mM * 1 mL in DMSO USD 192 In-stock
Solid
5 mg USD 170 In-stock
10 mg USD 260 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Ned 19:

Ned 19 Related Antibodies

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Description

Ned 19 is a selective membrane-permeant non competitive NAADP antagonist and inhibits NAADP-mediated Ca2+ signaling, with an IC50 of 65 nM[1]. Ned 19 strongly inhibits tumor growth and vascularization as well as lung metastases in mice[2].

IC50 & Target

NAADP, Ca2+[1]
In Vitro

Ned 19 (25-100 μM; 24-72 hours) reduces cell proliferation[2].
Ned 19 (25-100 μM; 24-72 hours) reduces markedly the cell number[2].
Ned 19 (25-100 μM; 24-72 hours) reduces the S phase percentage and increases of the G0/G1 phase percentage evaluated by cell cycle analysis[2].
Ned 19 (25-100 μM; 24-72 hours) induces cell apoptosis a time-dependent manner[2].
Ned 19 (25-100 μM; 24-72 hours) reduces expression of N-cadherin and increases expression of E-cadherin, affecting the cell migratory behavior[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ned 19 Related Antibodies

Cell Proliferation Assay[2]

Cell Line: B16 cells
Concentration: 25, 50, 100 μM
Incubation Time: 24, 48, 72 hours
Result: Reduced cell proliferation.

Cell Viability Assay[2]

Cell Line: B16 cells
Concentration: 25, 50, 100 μM
Incubation Time: 24, 48, 72 hours
Result: Reduced markedly the cell number.

Cell Cycle Analysis[2]

Cell Line: B16 cells
Concentration: 25, 50, 100 μM
Incubation Time: 24, 48, 72 hours
Result: Reduced the S phase percentage and increased of the G0/G1 phase percentage.

Apoptosis Analysis[2]

Cell Line: B16 cells
Concentration: 25, 50, 100 μM
Incubation Time: 24, 48, 72 hours
Result: Induced cell apoptosis a time-dependent manner.

Western Blot Analysis[2]

Cell Line: B16 cells
Concentration: 25, 50, 100 μM
Incubation Time: 24, 48, 72 hours
Result: Reduced expression of N-cadherin and increased expression of E-cadherin.
In Vivo

Ned 19 (i.p.; 5 mg/Kg/every second day; 4 week) impaires severely tumor growth[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male C57BL/6 mice[2]
Dosage: 5 mg/Kg
Administration: I.p.; every second day; 4 week
Result: Impaired severely tumor growth.
Molecular Weight

514.59

Formula

C30H31FN4O3
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

OC(C(C1)NC(C2=CC=C(OC)C(CN3CCN(C4=CC=CC=C4F)CC3)=C2)C5=C1C6=C(N5)C=CC=C6)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (242.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9433 mL 9.7165 mL 19.4329 mL
5 mM 0.3887 mL 1.9433 mL 3.8866 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9433 mL 9.7165 mL 19.4329 mL 48.5824 mL
5 mM 0.3887 mL 1.9433 mL 3.8866 mL 9.7165 mL
10 mM 0.1943 mL 0.9716 mL 1.9433 mL 4.8582 mL
15 mM 0.1296 mL 0.6478 mL 1.2955 mL 3.2388 mL
20 mM 0.0972 mL 0.4858 mL 0.9716 mL 2.4291 mL
25 mM 0.0777 mL 0.3887 mL 0.7773 mL 1.9433 mL
30 mM 0.0648 mL 0.3239 mL 0.6478 mL 1.6194 mL
40 mM 0.0486 mL 0.2429 mL 0.4858 mL 1.2146 mL
50 mM 0.0389 mL 0.1943 mL 0.3887 mL 0.9716 mL
60 mM 0.0324 mL 0.1619 mL 0.3239 mL 0.8097 mL
80 mM 0.0243 mL 0.1215 mL 0.2429 mL 0.6073 mL
100 mM 0.0194 mL 0.0972 mL 0.1943 mL 0.4858 mL
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Ned 19 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ned 19874374-25-1Ned19Ned-19Calcium ChannelCa2+ channelsCa channelsInhibitorinhibitorinhibit

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