Cat. No.: HY-103316A

Ned 19

3 Cited Publications

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Based on 3 publication(s) in Google Scholar

Ned 19 is a selective membrane-permeant non competitive NAADP antagonist and inhibits NAADP-mediated Ca²⁺ signaling, with an IC₅₀ of 65 nM. Ned 19 strongly inhibits tumor growth and vascularization as well as lung metastases in mice.

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Pureté: 99.92%
CAS No.: 874374-25-1
Formule: C₃₀H₃₁FN₄O₃
Masse moléculaire:514.59

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Ned 19 Chemical Structure

CAS No.: 874374-25-1

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvant et solubilité
- In Vitro
Pureté et documentation
Références
Help & FAQs
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Activité biologique

Description

Ned 19 is a selective membrane-permeant non competitive NAADP antagonist and inhibits NAADP-mediated Ca²⁺ signaling, with an IC₅₀ of 65 nM^[1]. Ned 19 strongly inhibits tumor growth and vascularization as well as lung metastases in mice^[2].

IC₅₀ & Target

NAADP, Ca₂₊^[1]

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In Vitro

Ned 19 (25-100 μM; 24-72 hours) reduces cell proliferation^[2].
Ned 19 (25-100 μM; 24-72 hours) reduces markedly the cell number^[2].
Ned 19 (25-100 μM; 24-72 hours) reduces the S phase percentage and increases of the G0/G1 phase percentage evaluated by cell cycle analysis^[2].
Ned 19 (25-100 μM; 24-72 hours) induces cell apoptosis a time-dependent manner^[2].
Ned 19 (25-100 μM; 24-72 hours) reduces expression of N-cadherin and increases expression of E-cadherin, affecting the cell migratory behavior^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:

B16 cells
Concentration:

25, 50, 100 μM
Incubation Time:

24, 48, 72 hours
Result:

Reduced cell proliferation.

Cell Viability Assay^[2]

Cell Line:

B16 cells
Concentration:

25, 50, 100 μM
Incubation Time:

24, 48, 72 hours
Result:

Reduced markedly the cell number.

Cell Cycle Analysis^[2]

Cell Line:

B16 cells
Concentration:

25, 50, 100 μM
Incubation Time:

24, 48, 72 hours
Result:

Reduced the S phase percentage and increased of the G0/G1 phase percentage.

Apoptosis Analysis^[2]

Cell Line:

B16 cells
Concentration:

25, 50, 100 μM
Incubation Time:

24, 48, 72 hours
Result:

Induced cell apoptosis a time-dependent manner.

Western Blot Analysis^[2]

Cell Line:

B16 cells
Concentration:

25, 50, 100 μM
Incubation Time:

24, 48, 72 hours
Result:

Reduced expression of N-cadherin and increased expression of E-cadherin.

In Vivo

Ned 19 (i.p.; 5 mg/Kg/every second day; 4 week) impaires severely tumor growth^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Adult male C57BL/6 mice^[2]
Dosage:

5 mg/Kg
Administration:

I.p.; every second day; 4 week
Result:

Impaired severely tumor growth.

Chemical Information

CAS No. 874374-25-1
Appearance Solid
Masse moléculaire 514.59
Formule C₃₀H₃₁FN₄O₃
Color Off-white to light yellow
SMILES

OC(C(C1)NC(C2=CC=C(OC)C(CN3CCN(C4=CC=CC=C4F)CC3)=C2)C5=C1C6=C(N5)C=CC=C6)=O
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (3)

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Journal Impact Factor

Nat Commun

A gain-of-function TPC2 variant R210C increases affinity to PI(3,5)P2 and causes lysosome acidification and hypopigmentation. [Abstract]

2023 Jan 14;14(1):226. PMID: 36641477
Adv Sci (Weinh)

Activation of Lysosomal Retrograde Transport Triggers TPC1-IP3R1 Ca2+ Crosstalk at Lysosome-ER MCSs Leading to Lethal Depleting of ER Calcium. [Abstract]

2025 Jul 25:e15313. PMID: 40709664
Cell Rep

Reduced lysosomal density in neuronal dendrites mediates deficits in synaptic plasticity in Huntington's disease. [Abstract]

2023 Dec 26;42(12):113573. PMID: 38096054

Solvant et solubilité

In Vitro:

DMSO : 125 mg/mL (242.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9433 mL

9.7165 mL

19.4329 mL

5 mM

0.3887 mL

1.9433 mL

3.8866 mL

10 mM

0.1943 mL

0.9716 mL

1.9433 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Rosen D, et al. Analogues of the nicotinic acid adenine dinucleotide phosphate (NAADP) antagonistNed-19 indicate two binding sites on the NAADP receptor. J Biol Chem. 2009 Dec 11;284(50):34930-4. [Content Brief]

[2]. Annarita Favia, et al. NAADP-Dependent Ca2+ Signaling Controls Melanoma Progression, Metastatic Dissemination and Neoangiogenesis. Sci Rep. 2016; 6: 18925.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9433 mL	9.7165 mL	19.4329 mL	48.5824 mL
	5 mM	0.3887 mL	1.9433 mL	3.8866 mL	9.7165 mL
	10 mM	0.1943 mL	0.9716 mL	1.9433 mL	4.8582 mL
	15 mM	0.1296 mL	0.6478 mL	1.2955 mL	3.2388 mL
	20 mM	0.0972 mL	0.4858 mL	0.9716 mL	2.4291 mL
	25 mM	0.0777 mL	0.3887 mL	0.7773 mL	1.9433 mL
	30 mM	0.0648 mL	0.3239 mL	0.6478 mL	1.6194 mL
	40 mM	0.0486 mL	0.2429 mL	0.4858 mL	1.2146 mL
	50 mM	0.0389 mL	0.1943 mL	0.3887 mL	0.9716 mL
	60 mM	0.0324 mL	0.1619 mL	0.3239 mL	0.8097 mL
	80 mM	0.0243 mL	0.1215 mL	0.2429 mL	0.6073 mL
	100 mM	0.0194 mL	0.0972 mL	0.1943 mL	0.4858 mL

Keywords

Ned 19

3 Cited Publications

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Ned 19

Membrane Transporter/Ion Channel Compound Library

Neuronal Signaling Compound Library

Anti-Cancer Compound Library

Anti-Cardiovascular Disease Compound Library

Neuroprotective Compound Library

Calcium Channel Blocker Library

Antihypertensive Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Ion Channel Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Ned 19

Voir tous les produits spécifiques à Isoform Calcium Channel

Activité biologique

Chemical Information

Publications (3)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Ned 19

Membrane Transporter/Ion Channel Compound Library

Neuronal Signaling Compound Library

Anti-Cancer Compound Library

Anti-Cardiovascular Disease Compound Library

Neuroprotective Compound Library

Calcium Channel Blocker Library

Antihypertensive Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Ion Channel Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Ned 19

Voir tous les produits spécifiques à Isoform Calcium Channel

Activité biologique

Chemical Information

Publications (3)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Ned 19 Related Classifications

Cat. No.: HY-103316A