1. Membrane Transporter/Ion Channel
    Neuronal Signaling
  2. Calcium Channel
  3. Ned 19

Ned 19 

Cat. No.: HY-103316A
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Ned 19 is a selective membrane-permeant non competitive NAADP antagonist and inhibits NAADP-mediated Ca2+ signaling, with an IC50 of 65 nM. Ned 19 strongly inhibits tumor growth and vascularization as well as lung metastases in mice.

For research use only. We do not sell to patients.

Ned 19 Chemical Structure

Ned 19 Chemical Structure

CAS No. : 874374-25-1

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Description

Ned 19 is a selective membrane-permeant non competitive NAADP antagonist and inhibits NAADP-mediated Ca2+ signaling, with an IC50 of 65 nM[1]. Ned 19 strongly inhibits tumor growth and vascularization as well as lung metastases in mice[2].

IC50 & Target[1]

Ca2+

 

In Vitro

Ned 19 (25-100 μM; 24-72 hours) reduces cell proliferation[2].
Ned 19 (25-100 μM; 24-72 hours) reduces markedly the cell number[2].
Ned 19 (25-100 μM; 24-72 hours) reduces the S phase percentage and increases of the G0/G1 phase percentage evaluated by cell cycle analysis[2].
Ned 19 (25-100 μM; 24-72 hours) induces cell apoptosis a time-dependent manner[2].
Ned 19 (25-100 μM; 24-72 hours) reduces expression of N-cadherin and increases expression of E-cadherin, affecting the cell migratory behavior[2].

Cell Proliferation Assay[2]

Cell Line: B16 cells
Concentration: 25, 50, 100 μM
Incubation Time: 24, 48, 72 hours
Result: Reduced cell proliferation.

Cell Viability Assay[2]

Cell Line: B16 cells
Concentration: 25, 50, 100 μM
Incubation Time: 24, 48, 72 hours
Result: Reduced markedly the cell number.

Cell Cycle Analysis[2]

Cell Line: B16 cells
Concentration: 25, 50, 100 μM
Incubation Time: 24, 48, 72 hours
Result: Reduced the S phase percentage and increased of the G0/G1 phase percentage.

Apoptosis Analysis[2]

Cell Line: B16 cells
Concentration: 25, 50, 100 μM
Incubation Time: 24, 48, 72 hours
Result: Induced cell apoptosis a time-dependent manner.

Western Blot Analysis[2]

Cell Line: B16 cells
Concentration: 25, 50, 100 μM
Incubation Time: 24, 48, 72 hours
Result: Reduced expression of N-cadherin and increased expression of E-cadherin.
In Vivo

Ned 19 (i.p.; 5 mg/Kg/every second day; 4 week) impaires severely tumor growth[2].

Animal Model: Adult male C57BL/6 mice[2]
Dosage: 5 mg/Kg
Administration: I.p.; every second day; 4 week
Result: Impaired severely tumor growth.
Molecular Weight

514.59

Formula

C₃₀H₃₁FN₄O₃

CAS No.

874374-25-1

SMILES

OC(C(C1)NC(C2=CC=C(OC)C(CN3CCN(C4=CC=CC=C4F)CC3)=C2)C5=C1C6=C(N5)C=CC=C6)=O

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Room temperature in continental US; may vary elsewhere

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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