Nek2-IN-5
Based on 1 Customer Validation
Nek2-IN-5 (compound 6) is a potent and irreversible Nek2 ((Never in mitosis gene a)-related kinase 2) inhibitior. Nek2-IN-5 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity : 99.17%
- CAS No.: 1507367-00-1
- Formula: C15H12N6O
- Molecular Weight:292.30
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
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NEK2 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | GI50 |
18.1 μM
Compound: 66
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Growth inhibition against human HCT116 cells incubated for 72 hrs
Growth inhibition against human HCT116 cells incubated for 72 hrs
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[PMID: 33479670] |
| HeLa | GI50 |
32.2 μM
Compound: 66
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Growth inhibition of human HeLa cells incubated for 72 hrs
Growth inhibition of human HeLa cells incubated for 72 hrs
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[PMID: 33479670] |
| HeLa | GI50 |
47.1 μM
Compound: 66
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Growth inhibition of human HeLa cells measured after 92 hrs by CellTiter Blue assay
Growth inhibition of human HeLa cells measured after 92 hrs by CellTiter Blue assay
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[PMID: 33479670] |
| MCF7 | GI50 |
6.6 μM
Compound: 66
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Growth inhibition against human MCF-7 cells incubated for 72 hrs
Growth inhibition against human MCF-7 cells incubated for 72 hrs
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[PMID: 33479670] |
| Rec1 | GI50 |
1.6 μM
Compound: 66
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Growth inhibition against human Rec-1 cells incubated for 72 hrs
Growth inhibition against human Rec-1 cells incubated for 72 hrs
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[PMID: 33479670] |
| SK-BR-3 | GI50 |
2.2 μM
Compound: 66
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Growth inhibition against human SKBR3 cells incubated for 72 hrs
Growth inhibition against human SKBR3 cells incubated for 72 hrs
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[PMID: 33479670] |
| T47D | GI50 |
3.6 μM
Compound: 66
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Growth inhibition against human T47D cells incubated for 72 hrs
Growth inhibition against human T47D cells incubated for 72 hrs
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[PMID: 33479670] |
| U2OS | GI50 |
71 μM
Compound: 66
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Growth inhibition of human U2OS cells measured after 92 hrs by CellTiter Blue assay
Growth inhibition of human U2OS cells measured after 92 hrs by CellTiter Blue assay
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[PMID: 33479670] |
Chemical Information
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CAS No. 1507367-00-1
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Appearance Solid
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Molecular Weight 292.30
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Formula C15H12N6O
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Color Light yellow to yellow
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SMILES
O=C(CC1=CC=CC(NC2=NC(C#C)=C3N=CNC3=N2)=C1)N
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 3.08 mg/mL (10.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4211 mL | 17.1057 mL | 34.2114 mL | 85.5286 mL |
| 5 mM | 0.6842 mL | 3.4211 mL | 6.8423 mL | 17.1057 mL | |
| 10 mM | 0.3421 mL | 1.7106 mL | 3.4211 mL | 8.5529 mL |
Nek2-IN-5 Related Classifications
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