2. Cell Cycle/DNA Damage
  3. NEKs Mitosis
  4. Nek2-IN-5

Nek2-IN-5 (compound 6) is a potent and irreversible Nek2 ((Never in mitosis gene a)-related kinase 2) inhibitior. Nek2-IN-5 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

Nek2-IN-5

Nek2-IN-5 Chemical Structure

CAS No. : 1507367-00-1

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Nek2-IN-5 Related Antibodies

Top Publications Citing Use of Products

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NEK1 NEK2 NEK4 NEK6 NEK7

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Description

Nek2-IN-5 (compound 6) is a potent and irreversible Nek2 ((Never in mitosis gene a)-related kinase 2) inhibitior[1]. Nek2-IN-5 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC50 & Target

NEK2

 

Cellular Effect
Cell Line Type Value Description References
HCT-116 GI50
18.1 μM
Compound: 66
Growth inhibition against human HCT116 cells incubated for 72 hrs
Growth inhibition against human HCT116 cells incubated for 72 hrs
[PMID: 33479670]
HeLa GI50
32.2 μM
Compound: 66
Growth inhibition of human HeLa cells incubated for 72 hrs
Growth inhibition of human HeLa cells incubated for 72 hrs
[PMID: 33479670]
HeLa GI50
47.1 μM
Compound: 66
Growth inhibition of human HeLa cells measured after 92 hrs by CellTiter Blue assay
Growth inhibition of human HeLa cells measured after 92 hrs by CellTiter Blue assay
[PMID: 33479670]
MCF7 GI50
6.6 μM
Compound: 66
Growth inhibition against human MCF-7 cells incubated for 72 hrs
Growth inhibition against human MCF-7 cells incubated for 72 hrs
[PMID: 33479670]
Rec1 GI50
1.6 μM
Compound: 66
Growth inhibition against human Rec-1 cells incubated for 72 hrs
Growth inhibition against human Rec-1 cells incubated for 72 hrs
[PMID: 33479670]
SK-BR-3 GI50
2.2 μM
Compound: 66
Growth inhibition against human SKBR3 cells incubated for 72 hrs
Growth inhibition against human SKBR3 cells incubated for 72 hrs
[PMID: 33479670]
T47D GI50
3.6 μM
Compound: 66
Growth inhibition against human T47D cells incubated for 72 hrs
Growth inhibition against human T47D cells incubated for 72 hrs
[PMID: 33479670]
U2OS GI50
71 μM
Compound: 66
Growth inhibition of human U2OS cells measured after 92 hrs by CellTiter Blue assay
Growth inhibition of human U2OS cells measured after 92 hrs by CellTiter Blue assay
[PMID: 33479670]
Molecular Weight

292.30

Formula

C15H12N6O
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(CC1=CC=CC(NC2=NC(C#C)=C3N=CNC3=N2)=C1)N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 3.08 mg/mL (10.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4211 mL 17.1057 mL 34.2114 mL
5 mM 0.6842 mL 3.4211 mL 6.8423 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4211 mL 17.1057 mL 34.2114 mL 85.5286 mL
5 mM 0.6842 mL 3.4211 mL 6.8423 mL 17.1057 mL
10 mM 0.3421 mL 1.7106 mL 3.4211 mL 8.5529 mL
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Nek2-IN-5 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nek2-IN-51507367-00-1NEKsMitosisNIMA–related KinasesNIMA (never in mitosis gene a)-related Expressed Kinases6-ethynylpurinecancercysteineInhibitorinhibitorinhibit

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