Cat. No.: HY-N0070

Solasonine

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Solasonine is a ferroptosis inducer which can be isolated from Solanum melongena that has anti-infection, anti-cancer, and neurogenesis promoting properties. Solasonine promotes ferroptosis of HCC cells via destruction of the glutathione redox system induced by inhibiting GPX4, and can be used for cancer research.

For research use only. We do not sell to patients.

Purity: 99.0%
CAS No.: 19121-58-5
Formula: C₄₅H₇₃NO₁₆
Molecular Weight:884.06

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solasonine Chemical Structure

CAS No.: 19121-58-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

GPX4

All Glutathione Peroxidase Isoforms :

GPX1
GPX4

Cellular Effect

Cell Line	Type	Value	Description	References
KB	ED₅₀	11.4 μg/mL Compound: solasonine	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 8450317]
PLC-PRF-5	ED₅₀	7.88 μg/mL Compound: solasonine	Cytotoxicity against human PLC/PRF/5 cells Cytotoxicity against human PLC/PRF/5 cells	[PMID: 8450317]

In Vitro

Solasonine (2.5-50 ng/mL, 24 h) inhibits cell viability of HepG2 and HepRG in a dose-dependent manner with IC₅₀s of about 15 ng/mL for both HepG2 and HepRG^[2].
Solasonine (15 ng/mL, 24 h) significantly increases the proportions of apoptotic HepG2 and HepRG cells from about 4% to more than 20 %^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 19121-58-5
Appearance Solid
Molecular Weight 884.06
Formula C₄₅H₇₃NO₁₆
Color White to light yellow
SMILES

C[C@H](CC1)CN[C@]21O[C@@]3([H])C[C@@]4([H])[C@]5([H])CC=C6C[C@@H](O[C@@]7([H])[C@H](O[C@@]8([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O8)[C@@H](O[C@@]9([H])[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O9)[C@@H](O)[C@@H](CO)O7)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@@]3([H])[C@@H]2C
Structure Classification
- Alkaloids
- Piperidine Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

All Cell Proliferation/Viability AssayCell Imaging/StainingFlow CytometryWBIF

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Journal Impact Factor

Front Oncol

Solasonine Causes Redox Imbalance and Mitochondrial Oxidative Stress of Ferroptosis in Lung Adenocarcinoma. [Abstract]

2022 May 18:12:874900. PMID: 35664792

Solasonine purchased from MedChemExpress. Usage Cited in: Front Oncol. 2022 May 18:12:874900. [Abstract]

The cell viability of Calu-1 and A549 cells was measured using the CCK-8 assay after SS treatment for 24 h.

Solasonine purchased from MedChemExpress. Usage Cited in: Front Oncol. 2022 May 18:12:874900. [Abstract]

Microscope observation of Calu-1 or A549 cells treated with 10, 15, 20 μM or 20, 25, 30 μM of Solasonine (SS) for 24 h, respectively.

Solasonine purchased from MedChemExpress. Usage Cited in: Front Oncol. 2022 May 18:12:874900. [Abstract]

Flow cytometry were used to detect ROS production in Calu-1 or A549 cells treated for 6 h with 10, 15, 20 μM or 20, 25, 30 μM Solasonine (SS) and pretreated with or without Fer-1 (1 μM).

Solasonine purchased from MedChemExpress. Usage Cited in: Front Oncol. 2022 May 18:12:874900. [Abstract]

Western blot analysis was used to detect the expressions of SLC7A11 and GPX4 in Calu-1 cells, which were treated with 10, 15, 20 μM Solasonine (SS) for 6 h.

Solasonine purchased from MedChemExpress. Usage Cited in: Front Oncol. 2022 May 18:12:874900. [Abstract]

Fluorescence microscopy was used to detect the lipid ROS production in Calu-1cells, which were treated with 20 μM Solasonine (SS) for 6 h and pretreated with or without Trolox (100 mM) or Mito TEMPO (10 mM).
Adv Biol (Weinh)

Solasonine Restores Sensitivity of Gastric Cancer to NK Cells through DNA Demethylation of MICA. [Abstract]

2025 Aug 7:e00793. PMID: 40772533
bioRxiv

2024 Mar 20.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (113.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.1311 mL

5.6557 mL

11.3114 mL

5 mM

0.2262 mL

1.1311 mL

2.2623 mL

10 mM

0.1131 mL

0.5656 mL

1.1311 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (2.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (2.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ding X, et al. Purification, antitumor activity in vitro of steroidal glycoalkaloids from black nightshade (Solanum nigrum L.). Food Chem. 2013 Nov 15;141(2):1181-6. [Content Brief]

[2]. Jin M, et al. Solasonine promotes ferroptosis of hepatoma carcinoma cells via glutathione peroxidase 4-induced destruction of the glutathione redox system. Biomed Pharmacother. 2020 Sep;129:110282. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.1311 mL	5.6557 mL	11.3114 mL	28.2786 mL
	5 mM	0.2262 mL	1.1311 mL	2.2623 mL	5.6557 mL
	10 mM	0.1131 mL	0.5656 mL	1.1311 mL	2.8279 mL
	15 mM	0.0754 mL	0.3770 mL	0.7541 mL	1.8852 mL
	20 mM	0.0566 mL	0.2828 mL	0.5656 mL	1.4139 mL
	25 mM	0.0452 mL	0.2262 mL	0.4525 mL	1.1311 mL
	30 mM	0.0377 mL	0.1885 mL	0.3770 mL	0.9426 mL
	40 mM	0.0283 mL	0.1414 mL	0.2828 mL	0.7070 mL
	50 mM	0.0226 mL	0.1131 mL	0.2262 mL	0.5656 mL
	60 mM	0.0189 mL	0.0943 mL	0.1885 mL	0.4713 mL
	80 mM	0.0141 mL	0.0707 mL	0.1414 mL	0.3535 mL
	100 mM	0.0113 mL	0.0566 mL	0.1131 mL	0.2828 mL

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Solasonine

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Glycoside Compound Library

Lipid Compound Library

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Traditional Chinese Medicine Active Compound Library

Alkaloids Library

Anti-Cancer Metabolism Compound Library

Food-Sourced Compound Library

Anti-Cancer Natural Product Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Cuproptosis Compound Library

Metabolic Enzyme Compound Library

Cell Death Library

Anti-Ovarian Cancer Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Miao Ethnicity Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Solasonine

All Glutathione Peroxidase Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Solasonine Related Classifications

HY-N0070 Related Classifications