2. Apoptosis
  3. TNF Receptor
  4. NMB04

NMB04 is a recombinant anti-Human CD257 (BAFF) (BLyS) antibody. NMB04 binds with specificity and high affinity to soluble and membrane-bound Blys, and blocks Blys binding to receptors TACI, BCMA, and BR-3. NMB04 can be used for the research of autoimmune diseases.

For research use only. We do not sell to patients.

NMB04

NMB04 Chemical Structure

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NMB04 Related Antibodies

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Description

NMB04 is a recombinant anti-Human CD257 (BAFF) (BLyS) antibody. NMB04 binds with specificity and high affinity to soluble and membrane-bound Blys, and blocks Blys binding to receptors TACI, BCMA, and BR-3. NMB04 can be used for the research of autoimmune diseases[1].

Species Reactivity

Human
In Vitro

NMB04 (1 h) binds to soluble human Blys with high affinity, as indicated by an EC50 of 0.023 nM[1].
NMB04 binds to soluble human Blys with a high sub-nanomolar affinity (KD = 7.38×10-10 M)[1].
NMB04 (1 h) binds to membrane-bound human Blys expressed on 293C18 cells with an EC50 of 0.13 nM[1].
NMB04 (1 h) potently inhibits Blys binding to human BR3, TACI, and BCMA receptors with IC50 values of 0.12 nM, 0.25 nM, and 0.12 nM respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NMB04 Related Antibodies
Parmacokinetics
Species Dose Route AUC0-∞ T1/2
Cynomolgus Monkey[1] 5 mg/kg i.v. 17456 μg·h/mL 153.83 h
In Vivo

NMB04 (5 mg/kg; i.v.; on days 1 and 3) completely inhibits human Blys-induced proliferation of mature B220+/ThB+ splenic B cells in BALB/C mice[1].
NMB04 (5 mg/kg; i.v.; once weekly; 4 weeks) inhibits CD20+ B cells in mesenteric lymph nodes and mature CD21+ B cells in peripheral blood in cynomolgus monkeys[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/C mice (female)[1]
Dosage: 5 mg/kg
Administration: i.v.; on days 1 and 3
Result: Completely attenuated the increase in B220+/ThB+ mature splenic B cell population induced by human Blys.
Reduced the proportion of B220+/ThB+ cells to levels comparable to control group.
Animal Model: Cynomolgus Monkeys (2-4 years old, male)[1]
Dosage: 5 mg/kg
Administration: i.v.; once weekly; 4 weeks
Result: Reduced the CD20+ B cell population in mesenteric lymph nodes to 18.31%.
Significantly inhibited the proliferation of mature CD21+ B cells in peripheral blood.
Gene ID

10673  [NCBI]

Accession
Target

TNFSF13B/BAFF/CD257
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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NMB04 Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NMB04NMB 04NMB-04TNF ReceptorTumor Necrosis Factor ReceptorTNFRBR-3TACIB lymphocyte stimulatorIM9 B cellcynomolgus monkeys293C18 cellsBCMABALB/C miceautoimmune diseasesBlysInhibitorinhibitorinhibit

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