IPTG
Based on 40 publication(s) in Google Scholar
IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 367-93-1
- Formula: C9H18O5S
- Molecular Weight:238.30
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) IPTG
More- Cell. 2026 Feb 5;189(3):853-871.e31. [Abstract]
- Cell Metab. 2026 May 13:S1550-4131(26)00150-6. [Abstract]
- Cell Metab. 2025 Jan 7;37(1):169-186.e9. [Abstract]
- Cancer Commun (Lond). 2025 Oct 16. [Abstract]
- Nat Biomed Eng. 2026 Jan 7.
- Cell Mol Immunol. 2025 Apr 14. [Abstract]
- Cell Host Microbe. 2024 Aug 14;32(8):1301-1314.e9. [Abstract]
- Cancer Res. 2025 Aug 21. [Abstract]
- Autophagy. 2026 Jun 3:1-31. [Abstract]
- Autophagy. 2025 May;21(5):917-933. [Abstract]
- Nat Commun. 2023 Oct 11;14(1):6384. [Abstract]
- J Adv Res. 2024 Dec 6:S2090-1232(24)00559-9. [Abstract]
- Theranostics. 2024 Jul 22;14(11):4481-4498. [Abstract]
- Acta Pharm Sin B. 2023 Mar;13(3):1071-1092. [Abstract]
- J Exp Clin Cancer Res. 2025 Aug 9;44(1):232. [Abstract]
- Sci Adv. 2023 Feb 17;9(7):eade4770. [Abstract]
- Cancer Lett. 2025 Dec 1:634:218072. [Abstract]
- Cell Commun Signal. 2025 Feb 5;23(1):65. [Abstract]
- Oncogene. 2023 Jul;42(29):2249-2262. [Abstract]
- Cell Chem Biol. 2026 Apr 16;33(4):523-537.e7. [Abstract]
- Sens Actuators B Chem. 2025 May 1.
- Free Radic Biol Med. 2025 Dec 24:245:43-55. [Abstract]
- ACS Appl Mater Interfaces. 2025 Oct 1;17(39):54423-54436. [Abstract]
- J Med Chem. 2026 Jan 23. [Abstract]
- J Ethnopharmacol. 2026 May 23:363:121390. [Abstract]
- EMBO Rep. 2025 Sep 22. [Abstract]
- Colloids Surf B Biointerfaces. 2025 Jul 22:255:114974. [Abstract]
- Int J Mol Sci. 2018 May 7;19(5):1393. [Abstract]
- Molecules. 2026 Feb 5;31(3):557. [Abstract]
- Aquaculture. 2026 Apr 24;622:744082.
- Mol Divers. 2025 May 3. [Abstract]
- Development. 2025 Nov 1;152(21):dev204701. [Abstract]
- Development. 2025 Oct 15;152(20):dev204483. [Abstract]
- AMB Express. 2023 Apr 29;13(1):40. [Abstract]
- J Sep Sci. 2026 Apr;49(4):e70402. [Abstract]
- Res Sq. 2026 May 21.
- bioRxiv. 2026 May 10:2026.05.04.722566. [Abstract]
- medRxiv. 2025 Nov 28.
- bioRxiv. 2025 Jan 2:2024.12.31.630902. [Abstract]
- bioRxiv. 2023 Jun 14.
Customer Validation & Images
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WB
Biological Activity
IPTG uptake by E. coli can be independent of the action of lactose permease, since other transport pathways are also involved. At low concentration, IPTG enters cells through lactose permease, but at high concentrations (typically used for protein induction), IPTG can enter the cells independently of lactose permease[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 367-93-1
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Appearance Solid
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Molecular Weight 238.30
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Formula C9H18O5S
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Color White to off-white
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SMILES
O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1SC(C)C
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Synonyms
Isopropyl β-D-thiogalactoside
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (40)
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Journal Impact Factor
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Most Recent
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Cell
Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2. [Abstract]2026 Feb 5;189(3):853-871.e31. PMID: 41576952 -
Cell Metab
Microbial phosphoketolase promotes histone lactylation to improve anti-TNF therapy efficacy in inflammatory bowel disease. [Abstract]2026 May 13:S1550-4131(26)00150-6. PMID: 42134325 -
Cell Metab
Microbiota-derived lysophosphatidylcholine alleviates Alzheimer's disease pathology via suppressing ferroptosis. [Abstract]2025 Jan 7;37(1):169-186.e9. PMID: 39510074 -
Cancer Commun (Lond)
RNA m1A methyltransferase TRMT61A promotes colorectal tumorigenesis by enhancing ONECUT2 mRNA stability and is a potential therapeutic target. [Abstract]2025 Oct 16. PMID: 41103012 -
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Cell Mol Immunol
The NET-DNA-CCDC25 inhibitor di-Pal-MTO suppresses tumor progression and promotes the innate immune response. [Abstract]2025 Apr 14. PMID: 40229592 -
Cell Host Microbe
2024 Aug 14;32(8):1301-1314.e9. PMID: 38996548 -
Cancer Res
The Acetyltransferase ARD1 Induces Glutathione Synthesis to Facilitate Ferroptosis Evasion in Hepatocellular Carcinoma. [Abstract]2025 Aug 21. PMID: 40838989 -
Autophagy
WNT2B impairs endosomal trafficking via WASHC5 to inhibit autophagy: a novel non-secretory WNT pathway. [Abstract]2026 Jun 3:1-31. PMID: 42233622 -
Autophagy
2025 May;21(5):917-933. PMID: 39710370 -
Nat Commun
Tripartite motif containing 26 prevents steatohepatitis progression by suppressing C/EBPδ signalling activation. [Abstract]2023 Oct 11;14(1):6384. PMID: 37821436 -
J Adv Res
Cancer-associated fibroblasts regulate mitochondrial metabolism and inhibit chemosensitivity via ANGPTL4-IQGAP1 axis in prostate cancer. [Abstract]2024 Dec 6:S2090-1232(24)00559-9. PMID: 39647634 -
Theranostics
Endosome-microautophagy targeting chimera (eMIATAC) for targeted proteins degradation and enhance CAR-T cell anti-tumor therapy. [Abstract]2024 Jul 22;14(11):4481-4498. PMID: 39113807 -
Acta Pharm Sin B
The deubiquitinating enzyme 13 retards non-alcoholic steatohepatitis via blocking inactive rhomboid protein 2-dependent pathway. [Abstract]2023 Mar;13(3):1071-1092. PMID: 36970206 -
J Exp Clin Cancer Res
Targeting NOTCH1-KEAP1 axis retards chronic liver injury and liver cancer progression via regulating stabilization of NRF2. [Abstract]2025 Aug 9;44(1):232. PMID: 40781716 -
Sci Adv
Imaging of Escherichia coli K5 and glycosaminoglycan precursors via targeted metabolic labeling of capsular polysaccharides in bacteria. [Abstract]2023 Feb 17;9(7):eade4770. PMID: 36800421 -
Cancer Lett
Targeting the FBXO5-DOK6 axis to overcome temozolomide resistance in glioblastoma via proteasome-cytomechanics regulation. [Abstract]2025 Dec 1:634:218072. PMID: 41045986 -
Cell Commun Signal
Chemo-proteomics reveals dihydrocaffeic acid exhibits anti-inflammation effects via Transaldolase 1 mediated PERK-NF-κB pathway. [Abstract]2025 Feb 5;23(1):65. PMID: 39910568 -
Oncogene
USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation. [Abstract]2023 Jul;42(29):2249-2262. PMID: 37311811 -
Cell Chem Biol
A nature-inspired nanoplatform for multi-protein targeted degradation via the autophagy-lysosome pathway. [Abstract]2026 Apr 16;33(4):523-537.e7. PMID: 41932333 -
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Free Radic Biol Med
Eleutheroside B ameliorates AD-like pathological features in Caenorhabditis elegans by inducing autophagy and combating oxidative stress. [Abstract]2025 Dec 24:245:43-55. PMID: 41453542 -
ACS Appl Mater Interfaces
Recombinant Trichosanthin-Loaded Nanoparticles with Tumor-Targeting and Cell-Penetrating Capabilities for Activatable Antitumor Therapy. [Abstract]2025 Oct 1;17(39):54423-54436. PMID: 40985241 -
J Med Chem
Discovery of 2-Chloro-pyrrolo[2,3- d]pyrimidin-4-one Derivatives as Protein Disulfide Isomerase Inhibitors with a Novel Allosteric-Covalent Binding Mode and Anti-Glioblastoma Activity. [Abstract]2026 Jan 23. PMID: 41574754 -
J Ethnopharmacol
2026 May 23:363:121390. PMID: 41707809 -
EMBO Rep
UBE2O-mediated ubiquitylation directs cytoplasmic CTNNA1 to promote cell-to-ECM adhesions. [Abstract]2025 Sep 22. PMID: 40983751 -
Colloids Surf B Biointerfaces
Precision targeting of ALK-positive lung cancer: Engineering HFN@MS4078 nanocages for optimized PROTAC delivery. [Abstract]2025 Jul 22:255:114974. PMID: 40712494 -
Int J Mol Sci
2018 May 7;19(5):1393. PMID: 29735940
IPTG purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2018 May 7;19(5):1393. [Abstract]
Screening of thymidylate synthase (TYMS) overexpression conditions.
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Molecules
Ginsenoside Rg5 Targets PRDX1 to Disrupt Redox Homeostasis and Induce Mitochondria-Dependent Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells. [Abstract]2026 Feb 5;31(3):557. PMID: 41683534 -
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Mol Divers
Unleashing the potential of traditional Chinese medicine: a computational approach to discovering drug targets utilizing the CSLN and molecular dynamics. [Abstract]2025 May 3. PMID: 40317435 -
Development
Antagonism between JAK/STAT downstream targets controls stem cell proliferation, cell fate conversion and tumorigenesis. [Abstract]2025 Nov 1;152(21):dev204701. PMID: 41051178 -
Development
2025 Oct 15;152(20):dev204483. PMID: 40521668 -
AMB Express
2023 Apr 29;13(1):40. PMID: 37119334 -
J Sep Sci
Oriented Immobilization Coupled With Ligand Fishing Strategy for Rapid Discovery of Direct HMGB1 Inhibitors in Taxus wallichiana var. mairei. [Abstract]2026 Apr;49(4):e70402. PMID: 41906428 -
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bioRxiv
2026 May 10:2026.05.04.722566. PMID: 42146548 -
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bioRxiv
Carbon source, cell density, and the microbial community control inhibition of V. cholerae surface colonization by environmental nitrate. [Abstract]2025 Jan 2:2024.12.31.630902. PMID: 39803477 -
Solvent & Solubility
H2O : 100 mg/mL (419.64 mM; Need ultrasonic)
DMSO : ≥ 60 mg/mL (251.78 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.73 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.73 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (419.64 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 4.1964 mL | 20.9820 mL | 41.9639 mL | 104.9098 mL |
| 5 mM | 0.8393 mL | 4.1964 mL | 8.3928 mL | 20.9820 mL | |
| 10 mM | 0.4196 mL | 2.0982 mL | 4.1964 mL | 10.4910 mL | |
| 15 mM | 0.2798 mL | 1.3988 mL | 2.7976 mL | 6.9940 mL | |
| 20 mM | 0.2098 mL | 1.0491 mL | 2.0982 mL | 5.2455 mL | |
| 25 mM | 0.1679 mL | 0.8393 mL | 1.6786 mL | 4.1964 mL | |
| 30 mM | 0.1399 mL | 0.6994 mL | 1.3988 mL | 3.4970 mL | |
| 40 mM | 0.1049 mL | 0.5245 mL | 1.0491 mL | 2.6227 mL | |
| 50 mM | 0.0839 mL | 0.4196 mL | 0.8393 mL | 2.0982 mL | |
| 60 mM | 0.0699 mL | 0.3497 mL | 0.6994 mL | 1.7485 mL | |
| 80 mM | 0.0525 mL | 0.2623 mL | 0.5245 mL | 1.3114 mL | |
| 100 mM | 0.0420 mL | 0.2098 mL | 0.4196 mL | 1.0491 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
IPTG Related Classifications
HY-15921 Related Classifications
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