Phenylarsine oxide
Based on 1 Customer Validation
Phenylarsine oxide (PAO), an inhibitor of endocytosis, inhibits PTPε with an IC50 of 18 μM. Phenylarsine oxide (PAO) inhibits oxygen consumption and decreases cellular ATP content overlap with those used to inhibit protein internalization.
For research use only. We do not sell to patients.
- Purity: 99.32%
- CAS No.: 637-03-6
- Formula: C6H5AsO
- Molecular Weight:168.02
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Metabolism/Protease Compound Library
HY-L012
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Phosphatase Inhibitor Library
HY-L081
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Metabolic Enzyme Compound Library
HY-L146
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Environmental Pollutant Compound Library
HY-L247
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | CC50 |
0.9 μM
Compound: Phenylarsine oxide
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Cytotoxicity against HEK293 cells
Cytotoxicity against HEK293 cells
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[PMID: 35178188] |
| HEK293 | IC50 |
1.49 μM
Compound: phenylarsine oxide
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Cytotoxicity against HEK cells after 16 hrs by resazurin-based fluorescence assay
Cytotoxicity against HEK cells after 16 hrs by resazurin-based fluorescence assay
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[PMID: 25007262] |
| HEK-293T | CC50 |
0.29 μM
Compound: PAO
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Cytostatic activity against HEK293T cells after 30 hrs by resazurin based fluorimetric assay
Cytostatic activity against HEK293T cells after 30 hrs by resazurin based fluorimetric assay
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[PMID: 28112515] |
| HEK-293T | CC50 |
0.32 μM
Compound: PAO
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Cytotoxicity against HEK293T cells after 30 hrs by Alamar Blue assay
Cytotoxicity against HEK293T cells after 30 hrs by Alamar Blue assay
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[PMID: 26231081] |
| HEK-293T | CC50 |
0.46 μM
Compound: Phenylarsine oxide
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Cytotoxicity against HEK293T cells assessed as reduction in cell viability incubated for 24 hrs by resazurin dye based fluorescence assay
Cytotoxicity against HEK293T cells assessed as reduction in cell viability incubated for 24 hrs by resazurin dye based fluorescence assay
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[PMID: 33940464] |
| HEK-293T | CC50 |
1.1 μM
Compound: PAO
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Cytotoxicity against HEK293T cells after 30 hrs by resazurin dye based fluorescence assay
Cytotoxicity against HEK293T cells after 30 hrs by resazurin dye based fluorescence assay
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[PMID: 30258542] |
| HEK-293T | EC50 |
6.4 μM
Compound: phenylarsine oxide
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Cytotoxicity against human HEK293T cells after 72 hrs by alamar blue assay
Cytotoxicity against human HEK293T cells after 72 hrs by alamar blue assay
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[PMID: 24900279] |
| HEK-293T | IC50 |
1.24 μM
Compound: Phenylarsine oxide
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Cytotoxicity against HEK293T cells after 16 hrs by alamar blue staining
Cytotoxicity against HEK293T cells after 16 hrs by alamar blue staining
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[PMID: 17698622] |
| HFF | CC50 |
0.29 μM
Compound: PAO
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Cytotoxicity against HFF cells assessed as reduction in cell viability after 30 hrs by resazurin-based assay
Cytotoxicity against HFF cells assessed as reduction in cell viability after 30 hrs by resazurin-based assay
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[PMID: 29544150] |
| NB-4 | IC50 |
0.03 μM
Compound: 11
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Antiproliferative activity against human NB4 cells expressing PML/RARalpha assessed as cell growth inhibition measured after 48 hrs by CCK-8 assay
Antiproliferative activity against human NB4 cells expressing PML/RARalpha assessed as cell growth inhibition measured after 48 hrs by CCK-8 assay
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[PMID: 38069817] |
Chemical Information
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CAS No. 637-03-6
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Appearance Solid
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Molecular Weight 168.02
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Formula C6H5AsO
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Color White to off-white
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SMILES
O=[As]C1=CC=CC=C1
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Synonyms
Oxophenylarsine; PhAsO; Arsenosobenzene
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 40 mg/mL (238.07 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 4 mg/mL (23.81 mM); Clear solution
This protocol yields a clear solution of ≥ 4 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 4 mg/mL (23.81 mM); Clear solution
This protocol yields a clear solution of ≥ 4 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (564 KB)
- English - EN (564 KB)
- Français - FR (564 KB)
- Deutsch - DE (564 KB)
- Norwegian - NO (564 KB)
- Español - ES (564 KB)
- Swedish - SV (564 KB)
- Italian - IT (564 KB)
- Portuguese - PT (564 KB)
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Handling Instructions (2659 KB)
References
[1]. A Schmidt, et al. Protein-tyrosine phosphatase activity regulates osteoclast formation and function: inhibition by alendronate. Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):3068-73. [Content Brief]
[2]. A E Gibson, et al. Phenylarsine oxide inhibition of endocytosis: effects on asialofetuin internalization. Am J Physiol. 1989 Aug;257(2 Pt 1):C182-4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.9517 mL | 29.7584 mL | 59.5167 mL | 148.7918 mL |
| 5 mM | 1.1903 mL | 5.9517 mL | 11.9033 mL | 29.7584 mL | |
| 10 mM | 0.5952 mL | 2.9758 mL | 5.9517 mL | 14.8792 mL | |
| 15 mM | 0.3968 mL | 1.9839 mL | 3.9678 mL | 9.9195 mL | |
| 20 mM | 0.2976 mL | 1.4879 mL | 2.9758 mL | 7.4396 mL | |
| 25 mM | 0.2381 mL | 1.1903 mL | 2.3807 mL | 5.9517 mL | |
| 30 mM | 0.1984 mL | 0.9919 mL | 1.9839 mL | 4.9597 mL | |
| 40 mM | 0.1488 mL | 0.7440 mL | 1.4879 mL | 3.7198 mL | |
| 50 mM | 0.1190 mL | 0.5952 mL | 1.1903 mL | 2.9758 mL | |
| 60 mM | 0.0992 mL | 0.4960 mL | 0.9919 mL | 2.4799 mL | |
| 80 mM | 0.0744 mL | 0.3720 mL | 0.7440 mL | 1.8599 mL | |
| 100 mM | 0.0595 mL | 0.2976 mL | 0.5952 mL | 1.4879 mL |
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