Phenylthiourea
Based on 3 publication(s) in Google Scholar
Phenylthiourea (Phenylthiocarbamide) is an inhibitor for phenoloxidase. Phenylthiourea inhibits enzymatic oxidation of DOPA by phenoloxidase (Ki = 0.21 μM). Phenylthiourea is an effective inhibitor for tyrosinase. Phenylthiourea can lead to graying of hair in black rats due to the interference with melanin formation.
For research use only. We do not sell to patients.
- Purity: 99.26%
- CAS No.: 103-85-5
- Formula: C7H8N2S
- Molecular Weight:152.22
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Phenylthiourea
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
phenoloxidase[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| B16 | IC50 |
1.8 μM
Compound: PTU
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Inhibition of tyrosinase in alpha-MSH-induced mouse B16 cells
Inhibition of tyrosinase in alpha-MSH-induced mouse B16 cells
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[PMID: 20638280] |
| B16 | IC50 |
5.4 μM
Compound: 1a
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Inhibition of melanogenesis in mouse B16 cells
Inhibition of melanogenesis in mouse B16 cells
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[PMID: 20359890] |
Phenylthiourea (Compound PTU) (0-20 μM, 0-300 s) affects the melanization process by either inhibiting the action of phenoloxidase directly or reducing DOPA-quinine to DOPA[1].
Phenylthiourea (0-2.5 μM) has inhibitory effect on DOPA oxidation catalyzed by phenoloxidase (Ki = 0.21 μM)[1].
Phenylthiourea (Compound Phenylthiocarbamide) inhibits the methemoglobin production in rat tissues[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 103-85-5
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Appearance Solid
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Molecular Weight 152.22
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Formula C7H8N2S
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Color White to off-white
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SMILES
S=C(NC1=CC=CC=C1)N
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Synonyms
Phenylthiocarbamide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Acta Pharmacol Sin
Discovery and characterization of a novel HIF-2α agonist for the treatment of CKD-related renal anemia. [Abstract]2025 Oct 1. PMID: 41034402 -
Chin Med
Targeting G-protein-coupled receptors and gut microbiota: Ge-Lian Qi-Shen decoction elevates GLP-1 to combat non-alcoholic fatty liver disease. [Abstract]2026 Jan 24;21(1):51. PMID: 41580820 -
Chin J Integr Med
Inhibitory Effects of Nardostachys Jatamansi DC. Volatile Oil on Psychological Factors SP/CORT-Induced Hyperpigmentation. [Abstract]2025 Dec;31(12):1097-1104. PMID: 41342982
Solvent & Solubility
DMSO : 100 mg/mL (656.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.42 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.42 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.5694 mL | 32.8472 mL | 65.6944 mL | 164.2360 mL |
| 5 mM | 1.3139 mL | 6.5694 mL | 13.1389 mL | 32.8472 mL | |
| 10 mM | 0.6569 mL | 3.2847 mL | 6.5694 mL | 16.4236 mL | |
| 15 mM | 0.4380 mL | 2.1898 mL | 4.3796 mL | 10.9491 mL | |
| 20 mM | 0.3285 mL | 1.6424 mL | 3.2847 mL | 8.2118 mL | |
| 25 mM | 0.2628 mL | 1.3139 mL | 2.6278 mL | 6.5694 mL | |
| 30 mM | 0.2190 mL | 1.0949 mL | 2.1898 mL | 5.4745 mL | |
| 40 mM | 0.1642 mL | 0.8212 mL | 1.6424 mL | 4.1059 mL | |
| 50 mM | 0.1314 mL | 0.6569 mL | 1.3139 mL | 3.2847 mL | |
| 60 mM | 0.1095 mL | 0.5475 mL | 1.0949 mL | 2.7373 mL | |
| 80 mM | 0.0821 mL | 0.4106 mL | 0.8212 mL | 2.0529 mL | |
| 100 mM | 0.0657 mL | 0.3285 mL | 0.6569 mL | 1.6424 mL |
Phenylthiourea Related Classifications
HY-Y0351 Related Classifications
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