2. Metabolic Enzyme/Protease NF-κB Neuronal Signaling Membrane Transporter/Ion Channel TGF-beta/Smad Epigenetics Immunology/Inflammation Apoptosis Cell Cycle/DNA Damage
  3. Angiotensin-converting Enzyme (ACE) IKK Calcium Channel PKC Reactive Oxygen Species (ROS) NF-κB Apoptosis Sirtuin NOD-like Receptor (NLR)
  4. Plantainoside D

Plantainoside D, a phenylethanoid glycosides, is a IKK-β inhibitor with diverse biological activities. Plantainoside D shows inhibitory activity of angiotensin-converting enzyme (ACE) with an IC50 of 2.17 mM. Plantainoside D significantly reduces the release of glutamate from nerve terminals in the cerebral cortex of rats by inhibiting the voltage-dependent calcium channel (VDCCs) and protein kinase C (PKC) signaling cascade. Plantainoside D significantly alleviates cell apoptosis by inhibiting the generation of ROS and the activation of NF-κB. Plantainoside D significantly improves acute lung injury (ALI) induced by sepsis by regulating the Sirt3/NLRP3 signaling pathway. Plantainoside D can be used for the study of neuroprotection, antioxidant, anti-inflammation, antihypertension.

For research use only. We do not sell to patients.

CAS No. : 147331-98-4

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Customer Review

Based on 2 publication(s) in Google Scholar

Plantainoside D Related Antibodies

Top Publications Citing Use of Products
IP
In Vivo Efficacy Study
Histological Imaging/Staining
IF
ELISA

    Plantainoside D purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Mar 25:82:103614.  [Abstract]

    Representative images of immunoblotting of GPX4 protein and its ubiquitination levels in HPMECs co-cultured with SMφ-EVs, with or without Plantainoside D (PD) (10 μM) treatment.

    Plantainoside D purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Mar 25:82:103614.  [Abstract]

    Survival rate of mice treated with or without CLP following administration of AAV-shGbp2 or Plantainoside D PD (30 mg/kg, i.v.) treatment.

    Plantainoside D purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Mar 25:82:103614.  [Abstract]

    Representative images of Evans blue staining and HE staining of mouse lungs treated with Plantainoside D PD (30 mg/kg, i.v.).

    Plantainoside D purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Mar 25:82:103614.  [Abstract]

    Representative images and quantification of Tunel staining of mouse lungs treated with Plantainoside D PD (30 mg/kg, i.v.).

    Plantainoside D purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Mar 25:82:103614.  [Abstract]

    Quantification of IL-6, TNF-α and IL-1β in mouse lungs treated with Plantainoside D PD (30 mg/kg, i.v.).

    View All IKK Isoform Specific Products:

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    IKK-α IKK-β IKK IKKε TBK1

    View All Calcium Channel Isoform Specific Products:

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    N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

    View All PKC Isoform Specific Products:

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    PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

    View All NF-κB Isoform Specific Products:

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    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    View All Sirtuin Isoform Specific Products:

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    Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7 SIRT4

    View All NOD-like Receptor (NLR) Isoform Specific Products:

    View All Isoforms
    NLRP3 NLRP1 NOD1 NOD2

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Plantainoside D, a phenylethanoid glycosides, is a IKK-β inhibitor with diverse biological activities. Plantainoside D shows inhibitory activity of angiotensin-converting enzyme (ACE) with an IC50 of 2.17 mM. Plantainoside D significantly reduces the release of glutamate from nerve terminals in the cerebral cortex of rats by inhibiting the voltage-dependent calcium channel (VDCCs) and protein kinase C (PKC) signaling cascade. Plantainoside D significantly alleviates cell apoptosis by inhibiting the generation of ROS and the activation of NF-κB. Plantainoside D significantly improves acute lung injury (ALI) induced by sepsis by regulating the Sirt3/NLRP3 signaling pathway. Plantainoside D can be used for the study of neuroprotection, antioxidant, anti-inflammation, antihypertension[1][2][3][4][5][6].

    IC50 & Target[1][2][5][6]

    IKK-β

     

    Calcium Channel

     

    NF-κB

     

    NLRP3

     

    ACE

    2.17 mM (IC50)

    PKC

     

    In Vitro

    Plantainoside D (0-50 μM, 10 min) significantly inhibits the release of glutamate induced by 4-aminopyridine (4-AP) (HY-B0604) via vesicle exocytosis pathway with an IC50 of 32 μM in rat synaptosome[1].
    Plantainoside D (30 μM, 10 min) reduces the Ca2+ influx induced by 4-AP through N-type calcium channels, and does not alter the membrane potential in rat synaptosome[1].
    Plantainoside D (30 μM, 20 min) acts through PKC-α/SNAP-25 phosphorylation as its key downstream mechanism in rat synaptosome[1].
    Plantainoside D (5 μM, 24 h) significantly improves sepsis induced ALI by regulation of Sirt3/NLRP3 pathway in MLE-12 cells[2].
    Plantainoside D (1-20 μg/mL, 1-4 h) significantly reduces the apoptosis induced by Doxorubicin (ADR) (HY-15142A) by inhibiting the generation of ROS and the activation of NF-κB[3].
    Plantainoside D exhibits weak inhibitory effects of CYP1A2 (IC50 = 12.83 μM), CYP2D6 (IC50 = 8.39 μM) and CYP3A4 (IC50 = 14.66 μM)[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Plantainoside D Related Antibodies

    Western Blot Analysis[1]

    Cell Line: Rat cerebral cortical synaptosomes
    Concentration: 30 μM
    Incubation Time: 20 min
    Result: Reduced the phosphorylation of PKC-α (from 239.2% to 54.1%) and SNAP-25 (from 228.9% to 89.7%) induced by 4-AP.

    Cell Viability Assay[2]

    Cell Line: MLE-12 cells induced by LPS (HY-D1056)
    Concentration: 5 μM
    Incubation Time: 24 h
    Result: Increased cell vitality, reduced ROS and MDA.

    Apoptosis Analysis[3]

    Cell Line: H9c2 cell with ADR
    Concentration: 1, 5, 10, 20 μg/mL
    Incubation Time: 1 h
    Result: Reduced the cell mortality rate with 20 μg/mL pretreatment.
    Reduced apoptotic morphology (nuclear enrichment, fragmentation).
    Increased Bcl-2/Bax ratio and inhibited caspase-3.

    Western Blot Analysis[3]

    Cell Line: H9c2 cell with ADR
    Concentration: 2 μM
    Incubation Time: 15 min, 30 min, 1h, 2h, 4h
    Result: Abrogated the activation of NF-κB.
    The glycoside reduced the degradation of IkBα in ADR-treated cells.
    In Vivo

    Plantainoside D (50 mg/kg, i.p., once daily for 7 days) significantly improves sepsis induced ALI by regulation of Sirt3/NLRP3 pathway in mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: LPS-induced ALI model established in ICR WT male mice (6 weeks, 16-18 g)[2]
    Dosage: 50 mg/kg
    Administration: Intraperitoneal injection (i.p.), once daily for 7 days
    Result: Significantly decreased the levels of w/d, ROS and MPO.
    Significantly decreased MDA and increased SOD and GSH-px in serum and lung tissues.
    Significantly restored oxidative stress and neutrophil infiltration.
    Significantly decreased the levels of cytokines TNF-α, IL-1β, IL-6, NLRP3, ASC and caspase-1.
    Molecular Weight

    640.59

    Formula

    C29H36O16
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O[C@H]([C@H]([C@@H]([C@@H](COC(/C=C/C1=CC=C(O)C(O)=C1)=O)O2)O)O[C@H]3[C@@H]([C@H]([C@@H]([C@@H](CO)O3)O)O)O)[C@@H]2OCCC4=CC=C(O)C(O)=C4
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 250 mg/mL (390.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5611 mL 7.8053 mL 15.6106 mL
    5 mM 0.3122 mL 1.5611 mL 3.1221 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (3.25 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (3.25 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
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    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.15%

    SDS (252 KB)

    COA (250 KB) HNMR (349 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.5611 mL 7.8053 mL 15.6106 mL 39.0265 mL
    5 mM 0.3122 mL 1.5611 mL 3.1221 mL 7.8053 mL
    10 mM 0.1561 mL 0.7805 mL 1.5611 mL 3.9027 mL
    15 mM 0.1041 mL 0.5204 mL 1.0407 mL 2.6018 mL
    20 mM 0.0781 mL 0.3903 mL 0.7805 mL 1.9513 mL
    25 mM 0.0624 mL 0.3122 mL 0.6244 mL 1.5611 mL
    30 mM 0.0520 mL 0.2602 mL 0.5204 mL 1.3009 mL
    40 mM 0.0390 mL 0.1951 mL 0.3903 mL 0.9757 mL
    50 mM 0.0312 mL 0.1561 mL 0.3122 mL 0.7805 mL
    60 mM 0.0260 mL 0.1301 mL 0.2602 mL 0.6504 mL
    80 mM 0.0195 mL 0.0976 mL 0.1951 mL 0.4878 mL
    100 mM 0.0156 mL 0.0781 mL 0.1561 mL 0.3903 mL
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    Plantainoside D Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Plantainoside D147331-98-4Angiotensin-converting Enzyme (ACE)IKKCalcium ChannelPKCReactive Oxygen Species (ROS)NF-κBApoptosisSirtuinNOD-like Receptor (NLR)IκB kinaseI kappa B kinase Ca2+ channelsCa channelsProtein kinase CNuclear factor-κBNuclear factor-kappaBGlutamate releaseVoltage-dependent Ca2+ channelCerebral cortexSynaptosomesInhibitorinhibitorinhibit

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