(+)-β-Pinene
(+)-β-Pinene (d-β-Pinene) acts as an Antifungal and Antibacterial agent. (+)-β-Pinene inhibits Phospholipase. (+)-β-Pinene interferes with fungal cell wall function, reduces Candida biofilm adhesion, inhibits Candida albicans biofilm formation, and suppresses the growth of various Candida species. Combined with Ciprofloxacin (HY-B0356), (+)-β-Pinene exerts synergistic antibacterial activity against Methicillin (HY-121544)-resistant Staphylococcus aureus. (+)-β-Pinene can be used in research related to candidiasis and Staphylococcus aureus infections.
For research use only. We do not sell to patients.
- CAS No.: 19902-08-0
- Formula: C10H16
- Molecular Weight:136.23
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
(+)-β-Pinene exhibits antifungal and anti-biofilm activity against 25 reference and clinical Candida spp. strains (including antifungal-susceptible and resistant isolates) with MIC values ranging from <56.25 to 1800 µmol/L, and acts primarily via cell wall interference rather than ergosterol binding[1].
(+)-β-Pinene exhibits microbicidal activity against Candida albicans ATCC 10231, Cryptococcus neoformans T1-444, Rhizopus oryzae UCP1506, and MRSA BMB9393 with MIC values of 187 µg/mL, 234 µg/mL, 780 µg/mL, and 6250 µg/mL, respectively[2].
(+)-β-Pinene (5 min-6 h) kills 100% of Candida albicans ATCC 10231 within 60 min and 100% of MRSA BMB9393 within 6 h when used at its respective MIC concentration[2].
(+)-β-Pinene (93.5-3125 µg/mL; 24 h) inhibits phospholipase activity by up to 37.91% and esterase activity by up to 72.6% in Cryptococcus neoformans T1-444, with minimal inhibition in Candida albicans ATCC 10231 and MRSA BMB9393[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 19902-08-0
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Molecular Weight 136.23
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Formula C10H16
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SMILES
CC1([C@@H]2C[C@H]1CCC2=C)C
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Synonyms
d-β-Pinene
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Fungal Biofilm Culture
Fungal biofilm culture is an in vitro method for growing surface-attached fungal communities, most commonly Candida albicans, on abiotic substrates such as polystyrene wells, silicone elastomer, or polymethylmethacrylate; the assay models adhesion, proliferation, filamentation, extracellular-matrix-associated maturation, and dispersion. Biofilm output can be read by optical density at 600 nm for adherent biomass, XTT reduction for metabolic activity, CFU recovery for viable attached or dispersed cells, and microscopy for architecture.
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Bacterial live/dead nucleic-acid viability staining
The LIVE/DEAD bacterial viability staining method is based on differential permeability of nucleic-acid-binding fluorescent dyes, most commonly SYTO 9 and propidium iodide (PI), which enables discrimination of bacterial populations with intact versus compromised cytoplasmic membranes. SYTO 9 penetrates both intact and damaged bacterial membranes and binds nucleic acids to produce green fluorescence, whereas propidium iodide penetrates only cells with compromised membranes and fluoresces red while also reducing SYTO 9 signal through competitive binding and fluorescence interactions. The resulting fluorescence pattern is interpreted as a proxy for membrane integrity, which is widely used as an indicator of bacterial viability in microscopy, flow cytometry, and spectroscopic platforms. However, mechanistic studies show that SYTO 9 and PI interactions involve displacement and fluorescence resonance energy transfer effects, which can influence signal interpretation depending on dye ratios a
Purity & Documentation
References
[1]. de Macêdo Andrade AC, et al. Antifungal Activity, Mode of Action, Docking Prediction and Anti-biofilm Effects of (+)-β-pinene Enantiomers against Candida spp. Current topics in medicinal chemistry. 2018;18(29):2481-2490. [Content Brief]
[2]. Rivas da Silva AC, et al. Biological activities of α-pinene and β-pinene enantiomers. Molecules (Basel, Switzerland). 2012 May 25;17(6):6305-16. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
(+)-β-Pinene Related Classifications
HY-W716339 Related Classifications
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