Poly(U)
Poly (U) (Polyuridylic acid) is an RNA chain that exists in a disordered extended state at room temperature and serves as a TLR7 ligand. Poly (U) produces distinguishable blocking effects when transported through α-hemolysin nanopores. Poly (U) forms a triple helix with adenosine or adenosine monophosphate, thereby significantly promoting the nucleotide bond condensation reaction between adenosine monophosphate and adenosine, while exerting minimal effects on other nucleosides. When conjugated with cationic lipids, Poly (U) specifically stimulates plasmacytoid dendritic cells to produce type I interferons.
For research use only. We do not sell to patients.
- CAS No.: 27416-86-0
- Formula: (C9H13N2O9P)x
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-101981
Uridine 5'-monophosphateNucleotide Analogue -
HY-101981R
Uridine 5'-monophosphate (Standard)Nucleotide Analogue -
HY-W416291
Polyadenylic acid potassiumPolyribonucleotide -
HY-W008940
Cytidine 5'-diphosphate disodium saltNucleotide -
HY-W008940R
Cytidine 5'-diphosphate disodium salt (Standard)Nucleotide -
HY-141567
Pseudouridine 5'-triphosphateUTP Derivative
Biological Activity
Description
IC50 & Target
[1]|
TLR7 |
In Vitro
Poly(U) (10 μg/mL) enabled α-hemolysin nanopores to distinguish 150 nt poly(U) from poly(A) via shorter translocation durations (centered at 1.4 μs/nt and 6 μs/nt) and absence of 55% amplitude blockade shoulders[1].
Poly(U) (30 μg/mL; 24 h) does not induce IFN-α or IL-12p40 production from Flt3L-induced murine bone marrow dendritic cells alone, but does induce both cytokines when mixed with Lipofectamine 2000, with IFN-α levels reaching ~10 ng/mL and IL-12p40 levels reaching ~4 ng/mL after 24 hours[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 27416-86-0
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Formula (C9H13N2O9P)x
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SMILES
O=C(C=CN1[C@H]2[C@H](O)[C@H](O)[C@@H](COP(O)(O)=O)O2)NC1=O.[n]
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Synonyms
Polyuridylic acid
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Monocyte-derived dendritic cell differentiation
Human monocyte-derived dendritic cells are generated by isolating PBMC-derived monocytes and culturing them with GM-CSF plus IL-4, which produces cells with dendritic-cell antigen-presenting properties, reduced monocyte phenotype, and increased dendritic-cell functional readouts such as antigen uptake, allogeneic T-cell stimulation, and expression of markers including HLA-DR, CD80, CD86, CD83, CD1a, or CD209 depending on protocol and maturation state. The main readout is phenotypic and functional differentiation: immature MoDCs are commonly evaluated by loss or reduction of CD14 with acquisition of dendritic-cell markers and antigen uptake capacity, whereas mature MoDCs are evaluated by increased CD83, CD80, CD86, HLA-DR, and T-cell stimulatory function after exposure to maturation stimuli such as TNF-α or a cytokine/PGE2 cocktail.
Purity & Documentation
References
[1]. Akeson M, et al. Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules[J]. Biophysical journal, 1999, 77(6): 3227-3233. [Content Brief]
[2]. Sugiyama T, et al. Immunoadjuvant effects of polyadenylic: polyuridylic acids through TLR3 and TLR7[J]. International immunology, 2008, 20(1): 1-9. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Poly(U) Related Classifications
HY-131920 Related Classifications
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