2. Metabolic Enzyme/Protease Apoptosis
  3. Endogenous Metabolite Ferroptosis
  4. Itaconic acid

Itaconic acid  (Synonyms: Methylenesuccinic acid)

Cat. No.: HY-Y0520 Purity: 99.88%
COA
SDS
Handling Instructions Technical Support

Itaconic acid, a precursor of polymers, chemicals, and fuels, can be synthesized by many fungi. Itaconic acid also is a macrophage-specific metabolite. Itaconic acid mediates crosstalk between macrophage metabolism and peritoneal tumors. Itaconic acid has anti-inflammatory, anti-microbial and immunomodulatory effect.

For research use only. We do not sell to patients.

CAS No. : 97-65-4

Size Price Stock Quantity
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Itaconic acid:

Itaconic acid Related Antibodies

Top Publications Citing Use of Products
Cell Imaging/Staining
Apoptosis Analysis
WB
Histological Imaging/Staining
In Vivo Efficacy Study

    Itaconic acid purchased from MedChemExpress. Usage Cited in: Apoptosis. 2025 Apr;30(3-4):991-1004.  [Abstract]

    Itaconic acid (Itaconate, IA; 80 μM). EDU method to detect the survival of HT22 cells.

    Itaconic acid purchased from MedChemExpress. Usage Cited in: Apoptosis. 2025 Apr;30(3-4):991-1004.  [Abstract]

    Itaconic acid (Itaconate, IA; 80 μM). Tunel to detect apoptosis of HT22 cells.

    Itaconic acid purchased from MedChemExpress. Usage Cited in: Apoptosis. 2025 Apr;30(3-4):991-1004.  [Abstract]

    Itaconic acid (Itaconate, IA; 80 μM). Representative western blot images and statistical analysis of ferroptosis-related proteins.

    Itaconic acid purchased from MedChemExpress. Usage Cited in: Apoptosis. 2025 Apr;30(3-4):991-1004.  [Abstract]

    Itaconic acid (Itaconate, IA; 80 μM). The effect of itaconate on lipid peroxidation level and Fe2+ level in HT22 cells.

    Itaconic acid purchased from MedChemExpress. Usage Cited in: Apoptosis. 2025 Apr;30(3-4):991-1004.  [Abstract]

    Itaconic acid (Itaconate, IA; 5 mg/kg; ip). Assessment of cell death in mice’s cortical brain tissue by HE staining.

    Itaconic acid purchased from MedChemExpress. Usage Cited in: Apoptosis. 2025 Apr;30(3-4):991-1004.  [Abstract]

    Itaconic acid (Itaconate, IA; 5 mg/kg; ip). Measurement of water content in the left hemisphere of mice’s brain by wet-dry weight method.

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Itaconic acid, a precursor of polymers, chemicals, and fuels, can be synthesized by many fungi. Itaconic acid also is a macrophage-specific metabolite. Itaconic acid mediates crosstalk between macrophage metabolism and peritoneal tumors. Itaconic acid has anti-inflammatory, anti-microbial and immunomodulatory effect[1][2][4][5].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Itaconic acid (2–8 mM, 24 h) induces ferroptosis by activating ferritinophagy in PANC1 cells[3].
    Itaconic acid (10 mM, 24 h) inhibits LPS-induced inflammatory cytokines in RAW264.7 cells[4].
    Itaconic acid (10 mM, 24 h) promotes the pentose phosphate pathway (PPP) and NADPH oxidase activity in RAW264.7 cells[4].
    Itaconic acid (10 mM, 24-72 h) inhibits cell viability of cerebellar granule cells (CGC) and inhibits ADP-stimulated respiration in permeabilized CGC cells[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Itaconic acid Related Antibodies
    Molecular Weight

    130.10

    Formula

    C5H6O4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(O)C(CC(O)=O)=C
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 27.78 mg/mL (213.53 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 7.6864 mL 38.4320 mL 76.8639 mL
    5 mM 1.5373 mL 7.6864 mL 15.3728 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 25 mg/mL (192.16 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.98%

    SDS (476 KB)

    COA (244 KB) HNMR (217 KB) RP-HPLC (241 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 7.6864 mL 38.4320 mL 76.8639 mL 192.1599 mL
    5 mM 1.5373 mL 7.6864 mL 15.3728 mL 38.4320 mL
    10 mM 0.7686 mL 3.8432 mL 7.6864 mL 19.2160 mL
    15 mM 0.5124 mL 2.5621 mL 5.1243 mL 12.8107 mL
    20 mM 0.3843 mL 1.9216 mL 3.8432 mL 9.6080 mL
    25 mM 0.3075 mL 1.5373 mL 3.0746 mL 7.6864 mL
    30 mM 0.2562 mL 1.2811 mL 2.5621 mL 6.4053 mL
    40 mM 0.1922 mL 0.9608 mL 1.9216 mL 4.8040 mL
    50 mM 0.1537 mL 0.7686 mL 1.5373 mL 3.8432 mL
    60 mM 0.1281 mL 0.6405 mL 1.2811 mL 3.2027 mL
    80 mM 0.0961 mL 0.4804 mL 0.9608 mL 2.4020 mL
    100 mM 0.0769 mL 0.3843 mL 0.7686 mL 1.9216 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Itaconic acid Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Itaconic acid97-65-4Methylenesuccinic acidEndogenous MetaboliteFerroptosispolymerschemicalsand fuelsfungimacrophageInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Itaconic acid
    Cat. No.:
    HY-Y0520
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (476 KB) Français - FR (476 KB) Deutsch - DE (476 KB) Norwegian - NO (476 KB) Español - ES (476 KB) Swedish - SV (476 KB) Italian - IT (476 KB) Korean - KR (476 KB) Portuguese - PT (476 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.