1. Epigenetics
  2. MicroRNA
  3. RJW100

RJW100 

Cat. No.: HY-131445
Handling Instructions

RJW100 is a potent liver receptor homolog 1 (LRH-1, NR5A2) and steroidogenic factor-1 (SF-1, NR5A1) agonist with pEC50s of 6.6 and 7.5, respectively. RJW100 also causes strong activation of the miR-200c (miRNA-200c, microRNA-200c) promoter.

For research use only. We do not sell to patients.

RJW100 Chemical Structure

RJW100 Chemical Structure

CAS No. : 1276664-20-0

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Description

RJW100 is a potent liver receptor homolog 1 (LRH-1, NR5A2) and steroidogenic factor-1 (SF-1, NR5A1) agonist with pEC50s of 6.6 and 7.5, respectively[1]. RJW100 also causes strong activation of the miR-200c (miRNA-200c, microRNA-200c) promoter[2].

IC50 & Target

pEC50: 6.6 (LRH-1) and 7.5 (SF-1)[1]

In Vitro

RJW100 (compound 24-exo) treatment induces a significant dose-dependent increase in small/short heterodimer partner (SHP) transcripts beginning at 5 µM[1].
RJW100 (compound 24-exo) clearly displaces the bound PIP2 phospholipid from SF-1 almost completely at 1 µM[1].
Using hLRH-1 LBD alone that had not been complexed with any phospholipids, the result shows a clear dose-dependent shift in hLRH-1 LBD (ligand binding domain) native PAGE migration upon RJW100 (compound 24-exo; 0-100 µM) binding[1].
RJW100 causes strong activation of the miR-200c promoter and exhibits strong ability to downregulate ZEB1 and ZEB2 proteins[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR[1]

Cell Line: HEK293 cells
Concentration: 1 µM, 5 µM, 10 µM
Incubation Time: 24 hours
Result: Induced a significant dose-dependent increase in SHP transcripts beginning at 5 µM.
Molecular Weight

386.57

Formula

C28H34O

CAS No.
SMILES

O[[email protected]@H]1CC[[email protected]@]2(C(C3=CC=CC=C3)=C)C(C4=CC=CC=C4)=C(CCCCCC)C[[email protected]@]12[H].[Relative stereochemistry]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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RJW100
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