1. Apoptosis
  2. MDM-2/p53
  3. RO-5963

RO-5963 

Cat. No.: HY-120086
Handling Instructions

RO-5963 is a dual p53-MDM2 and p53-MDMX inhibitor with IC50s of ~17 nM and ~24 nM, respectively.

For research use only. We do not sell to patients.

RO-5963 Chemical Structure

RO-5963 Chemical Structure

CAS No. : 1416663-77-8

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Description

RO-5963 is a dual p53-MDM2 and p53-MDMX inhibitor with IC50s of ~17 nM and ~24 nM, respectively[1].

IC50 & Target

IC50: ~17 nM (p53-MDM2), ~24 nM (p53-MDMX)[1]

In Vitro

RO-5963 (10-20 μM; 48 hours) shows much higher apoptotic activity than Nutlin in both MCF7 and ZR75-30 cell lines[1].
RO-5963 (10 μM, 24 hours) effectively activats p53 and elevates p21 and MDM2 levels[1].

Apoptosis Analysis[1]

Cell Line: ZR75-30, MCF7 cells
Concentration: 10, 20 μM
Incubation Time: 48 hours
Result: Potently showed apoptotic activity.

Western Blot Analysis[1]

Cell Line: LNCap, U2OS, RKO, A489, 22Rv1, HCT116, H460, LOX, MCF7, A549, G401 cells
Concentration: 10 μM
Incubation Time: 24 hour
Result: Effectively activated p53 and elevated p21 and MDM2 levels.
Molecular Weight

518.90

Formula

C₂₄H₂₁ClF₂N₄O₅

CAS No.

1416663-77-8

SMILES

OCC(CO)NC(C(C1=CC(F)=C(F)C=C1)N2C(/C(NC2=O)=C\C(C3=CC=C4Cl)=CNC3=C4C)=O)=O

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Room temperature in continental US; may vary elsewhere

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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