1. Apoptosis
    Metabolic Enzyme/Protease
  2. MDM-2/p53
    E1/E2/E3 Enzyme
  3. RO-5963


Cat. No.: HY-120086
Handling Instructions

RO-5963 is a dual p53-MDM2 and p53-MDMX inhibitor with IC50s of ~17 nM and ~24 nM, respectively.

For research use only. We do not sell to patients.

RO-5963 Chemical Structure

RO-5963 Chemical Structure

CAS No. : 1416663-77-8

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RO-5963 is a dual p53-MDM2 and p53-MDMX inhibitor with IC50s of ~17 nM and ~24 nM, respectively[1].

IC50 & Target

IC50: ~17 nM (p53-MDM2), ~24 nM (p53-MDMX)[1]

In Vitro

RO-5963 (10-20 μM; 48 hours) shows much higher apoptotic activity than Nutlin in both MCF7 and ZR75-30 cell lines[1].
RO-5963 (10 μM, 24 hours) effectively activats p53 and elevates p21 and MDM2 levels[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis[1]

Cell Line: ZR75-30, MCF7 cells
Concentration: 10, 20 μM
Incubation Time: 48 hours
Result: Potently showed apoptotic activity.

Western Blot Analysis[1]

Cell Line: LNCap, U2OS, RKO, A489, 22Rv1, HCT116, H460, LOX, MCF7, A549, G401 cells
Concentration: 10 μM
Incubation Time: 24 hour
Result: Effectively activated p53 and elevated p21 and MDM2 levels.
Molecular Weight







Room temperature in continental US; may vary elsewhere.


Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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