CP61
CP61 is a CtBP1 and CtBP2 inhibitor that binds to dimeric CtBP1 and allosterically inhibits NADH binding, disrupting the NADH-dependent dimerization of CtBP1 and CtBP2, inducing aberrant mitosis and micronucleus formation, thereby inhibiting cell proliferation and colony formation. CP61 can be used in breast cancer research.
For research use only. We do not sell to patients.
- CAS No.: 1631984-43-4
- Formula: C50H73N13O12S
- Molecular Weight:1080.26
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
CtBP1 |
CtBP2 |
In Vitro
CP61 acetate disrupts the NADH-dependent homodimerization of CtBP1[1].
CP61 acetate inhibits CtBP2 homodimerization with an IC50 of 19 μM in ELISA assays[1].
CP61 acetate disrupts CtBP1 dimerization in a dose-dependent manner[1].
CP61 acetate inhibits NADH binding to CtBP1 through an allosteric mechanism and binds to dimeric CtBP1 with an affinity of 3-11 μM[1].
CP61 (50 μM) acetate increases abnormal mitosis and prolongs mitotic duration in synchronized MCF-7 cells[1].
CP61 (50 μM) acetate selectively increases abnormal mitosis and prolongs mitotic duration in highly glycolytic MCF-7 cells, but has no such effect in MDA-MB-453 cells with lower glycolysis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7
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Concentration:50 μM
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Incubation Time:48 h
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Result:Did not affect the protein abundance of CtBP1 or CtBP2 in MCF-7 cells when treated with CP61-TAT.
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Breast Cancer Modeling
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Soft Agar Colony Formation Assay
- MTT Cell Proliferation Assay
- Genotoxicity/Mutagenicity Study
Chemical Information
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CAS No. 1631984-43-4
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Molecular Weight 1080.26
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Formula C50H73N13O12S
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Sequence
cyclo(Ser-Gly-Trp-Thr-Val-Val-Arg-Met-Tyr)
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Sequence Shortening
cyclo(SGWTVVRMY)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CP61 Related Classifications
HY-P10812 Related Classifications
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