Sec61-IN-2
Based on 1 Customer Validation
Sec61-IN-2 is a Sec61 inhibitor. Sec61-IN-2 shows a significant inhibitory effect on PD-1 related reporter gene cell models (PD1tsGluc) (IC50 = 42 nM), while it has almost no inhibitory activity in TNFα, Her3, and IL-2 pathway models. Sec61-IN-2 can be used for cancer research.
For research use only. We do not sell to patients.
- Purity: 99.51%
- CAS No.: 2484865-72-5
- Formula: C22H19N5OS
- Molecular Weight:401.48
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Immunology/Inflammation Compound Library
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Anti-Breast Cancer Compound Library
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Anti-Lung Cancer Compound Library
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Anti-Blood Cancer Compound Library
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Anti-Liver Cancer Compound Library
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Anti-Colorectal Cancer Compound Library
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Protein-protein Interaction Inhibitor Library
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Cancer Stem Cells Compound Library
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Heterocyclic Compound Library
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Membrane Protein-targeted Compound Library
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Membrane Receptor-targeted Compound Library
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Anti-Hematopathy Compound Library
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Anti-Ovarian Cancer Compound Library
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Bioactive Compound Library Max
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MCE Bioactive Compound Library
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Bioactive Compound Library
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Anti-Gastric Cancer Compound Library
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Anti-Brain Cancer Compound Library
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High-Throughput Bioactive Compound Library
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Biological Activity
Sec61-IN-2 (Compound A347) (48 h) exhibits EC50 values greater than 20 µM in viability assays using multiple myeloma cell lines H929 and U266[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2484865-72-5
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Appearance Solid
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Molecular Weight 401.48
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Formula C22H19N5OS
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Color Off-white to yellow
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SMILES
O=C(C1=CC=CN1CC2=CC=NC=C2)NC3=NC(/C=C/C4=NC=CC=C4C)=CS3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (249.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4908 mL | 12.4539 mL | 24.9078 mL | 62.2696 mL |
| 5 mM | 0.4982 mL | 2.4908 mL | 4.9816 mL | 12.4539 mL | |
| 10 mM | 0.2491 mL | 1.2454 mL | 2.4908 mL | 6.2270 mL | |
| 15 mM | 0.1661 mL | 0.8303 mL | 1.6605 mL | 4.1513 mL | |
| 20 mM | 0.1245 mL | 0.6227 mL | 1.2454 mL | 3.1135 mL | |
| 25 mM | 0.0996 mL | 0.4982 mL | 0.9963 mL | 2.4908 mL | |
| 30 mM | 0.0830 mL | 0.4151 mL | 0.8303 mL | 2.0757 mL | |
| 40 mM | 0.0623 mL | 0.3113 mL | 0.6227 mL | 1.5567 mL | |
| 50 mM | 0.0498 mL | 0.2491 mL | 0.4982 mL | 1.2454 mL | |
| 60 mM | 0.0415 mL | 0.2076 mL | 0.4151 mL | 1.0378 mL | |
| 80 mM | 0.0311 mL | 0.1557 mL | 0.3113 mL | 0.7784 mL | |
| 100 mM | 0.0249 mL | 0.1245 mL | 0.2491 mL | 0.6227 mL |
Sec61-IN-2 Related Classifications
HY-139616 Related Classifications
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