Spinosyn A

Cat. No.: HY-B0767 Purity: 99.93%: Data Sheet
COA

SDS
Handling Instructions
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Spinosyn A, a polyketide-derived macrolide produced by Saccharopolyspora spinosa, is a potent insecticide.

For research use only. We do not sell to patients.

Spinosyn A Chemical Structure

CAS No. : 131929-60-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Other Forms of Spinosyn A:

Spinosyn D In-stock
Spinosad In-stock

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Spinosyn A, a polyketide-derived macrolide produced by Saccharopolyspora spinosa, is a potent insecticide^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	10.7 μM Compound: 1	Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay	[PMID: 30201293]
HCT-116	IC₅₀	11.1 μM Compound: 1	Antiproliferative activity against human HCT116 cells incubated for 48 hrs Antiproliferative activity against human HCT116 cells incubated for 48 hrs	[PMID: 32139325]
HT-29	IC₅₀	7.3 μM Compound: SPA	Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay	[PMID: 37682035]
MCF7	IC₅₀	16.7 μM Compound: 1	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 30201293]
MCF7	IC₅₀	17.9 μM Compound: 1	Antiproliferative activity against human MCF7 cells incubated for 48 hrs Antiproliferative activity against human MCF7 cells incubated for 48 hrs	[PMID: 32139325]
NCI-H460	IC₅₀	66 μM Compound: 1	Antiproliferative activity against human NCI-H460 cells after 48 hrs by MTT assay Antiproliferative activity against human NCI-H460 cells after 48 hrs by MTT assay	[PMID: 30201293]
NCI-H460	IC₅₀	67.5 μM Compound: 1	Antiproliferative activity against human NCI-H460 cells incubated for 48 hrs Antiproliferative activity against human NCI-H460 cells incubated for 48 hrs	[PMID: 32139325]
RKO	IC₅₀	17 μM Compound: SPA	Antiproliferative activity against human RKO cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay Antiproliferative activity against human RKO cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay	[PMID: 37682035]
U-87MG ATCC	IC₅₀	103.8 μM Compound: 1	Antiproliferative activity against human U87MG cells after 48 hrs by MTT assay Antiproliferative activity against human U87MG cells after 48 hrs by MTT assay	[PMID: 30201293]
U-87MG ATCC	IC₅₀	96.5 μM Compound: 1	Antiproliferative activity against human U87MG cells incubated for 48 hrs Antiproliferative activity against human U87MG cells incubated for 48 hrs	[PMID: 32139325]

In Vitro

Spinosad is a mixture of two natural occurring macrocyclic lactones (spinosyn A and spinosyn D) and acts primarily as a nAChR agonist^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

731.96

Formula

C₄₁H₆₅NO₁₀

CAS No.

131929-60-7

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@H]([C@H](CCC[C@@H](O1)CC)O[C@@H]2O[C@@H]([C@H](CC2)N(C)C)C)C)C3=C[C@@]([C@](C=C[C@]4([H])C5)([H])[C@]3([H])CC1=O)([H])[C@]4([H])C[C@@H]5O[C@H]6[C@@H]([C@@H]([C@H]([C@@H](O6)C)OC)OC)OC

Structure Classification

Initial Source

Microorganisms

Saccharopolyspora spinosa

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (136.62 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3662 mL	6.8310 mL	13.6619 mL
5 mM	0.2732 mL	1.3662 mL	2.7324 mL
10 mM	0.1366 mL	0.6831 mL	1.3662 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (274 KB) SDS (597 KB)

COA (255 KB) HNMR (301 KB) RP-HPLC (255 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Hak Joong Kim, et al. Biosynthesis of spinosyn in Saccharopolyspora spinosa: synthesis of permethylated rhamnose and characterization of the functions of SpnH, SpnI, and SpnK. J Am Chem Soc. 2010 Mar 10;132(9):2901-3. [Content Brief]

[2]. D T Vo, et al. Insect nicotinic acetylcholine receptor agonists as flea adulticides in small animals. J Vet Pharmacol Ther. 2010 Aug;33(4):315-22. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3662 mL	6.8310 mL	13.6619 mL	34.1549 mL
	5 mM	0.2732 mL	1.3662 mL	2.7324 mL	6.8310 mL
	10 mM	0.1366 mL	0.6831 mL	1.3662 mL	3.4155 mL
	15 mM	0.0911 mL	0.4554 mL	0.9108 mL	2.2770 mL
	20 mM	0.0683 mL	0.3415 mL	0.6831 mL	1.7077 mL
	25 mM	0.0546 mL	0.2732 mL	0.5465 mL	1.3662 mL
	30 mM	0.0455 mL	0.2277 mL	0.4554 mL	1.1385 mL
	40 mM	0.0342 mL	0.1708 mL	0.3415 mL	0.8539 mL
	50 mM	0.0273 mL	0.1366 mL	0.2732 mL	0.6831 mL
	60 mM	0.0228 mL	0.1138 mL	0.2277 mL	0.5692 mL
	80 mM	0.0171 mL	0.0854 mL	0.1708 mL	0.4269 mL
	100 mM	0.0137 mL	0.0683 mL	0.1366 mL	0.3415 mL

Spinosyn A Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Spinosyn A131929-60-7InsecticideInhibitorinhibitorinhibit

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