Sulfamethoxazole 1000 µg/mL in methanol
Based on 24 publication(s) in Google Scholar
Sulfamethoxazole (Ro 4-2130) 1000 μg/mL in methanol is a sulfonamide antibiotic with a widespread antibacterial activity. Sulfamethoxazole 1000 μg/mL in methanol inhibits bacterial folate metabolism by competing with 4-Aminobenzoic acid (HY-B1008) (PABA) to act on dihydropteroate synthetase and dihydropteroate reductase. Sulfamethoxazole 1000 μg/mL in methanol can be used for the study of urinary tract infections (UTIs), prostatitis, and bronchitis.
For research use only. We do not sell to patients.
- CAS No.: 723-46-6
- Formula: C10H11N3O3S
- Molecular Weight:253.28
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-B0322
SulfamethoxazoleSulfonamide Antibiotic -
HY-B0322R
Sulfamethoxazole (Standard)Standard of Sulfamethoxazole -
HY-B1659
GlycerolEndogenous Metabolite -
HY-B0507
SulfathiazoleAntibiotics -
HY-B0035
SulfamethazineSulfonamide Antibiotic -
HY-N0565B
Doxycycline hyclateMMP Inhibitor -
HY-B0129
AztreonamBacterial Inhibitor -
HY-B0035R
Sulfamethazine (Standard)Reference Standard -
HY-B1659R
Glycerol (Standard)Endogenous Metabolite -
HY-B0333
SulfamethizoleSulfathiazole Antibiotic -
HY-B1455
ClindamycinLincosamide Antibiotic -
HY-B0273
SulfadiazineSulfonamide Antibiotic
Publications Citing Use of MedChemExpress (MCE) Sulfamethoxazole 1000 µg/mL in methanol
More- Signal Transduct Target Ther. 2023 Sep 18;8(1):353. [Abstract]
- Autophagy. 2023 Oct;19(10):2702-2718. [Abstract]
- Water Res. 2023 Jul 15:240:120110. [Abstract]
- J Exp Med. 2026 Mar 2;223(3):e20241287. [Abstract]
- ACS Environ Au. 2025 Aug 5;5(6):573-582. [Abstract]
- Food Chem X. 2026 Feb 8:34:103646. [Abstract]
- Clin Chem. 2019 Dec;65(12):1522-1531. [Abstract]
- Emerg Contam. 2026 Feb 23.
- Anal Chem. 2025 Jun 3;97(21):11099-11109. [Abstract]
- Pharmaceutics. 2025 Mar 26;17(4):423. [Abstract]
- Virulence. 2026 Dec 31;17(1):2646808. [Abstract]
- Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]
- Antibiotics (Basel). 2026 Mar 10;15(3):283. [Abstract]
- Clean Technol Environ Policy. 2025 May 22.
- Bioresour Technol Rep. 2023 Dec, 24, 101619.
- Microorganisms. 2025 Apr 18;13(4):938. [Abstract]
- Water. 2025 Jun 6.
- Infect Drug Resist. 2026 May 8;19.
- Eur J Clin Microbiol Infect Dis. 2025 Sep 29. [Abstract]
- PLoS One. 2026 Jul 8;21(7):e0352997.
- Res Sq. 2026 Mar 9.
- bioRxiv. 2025 January 19.
- bioRxiv. 2024 May 10.
- Chemosphere. 2019 Jun:225:378-387. [Abstract]
Customer Validation & Images
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
All Antibiotic Isoforms
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A7R5 | IC50 |
333 μM
Compound: 31
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Displacement of 125I-ET1 from ETA receptor in rat A7R5 cells
Displacement of 125I-ET1 from ETA receptor in rat A7R5 cells
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[PMID: 27321813] |
| A7R5 | IC50 |
333 μM
Compound: 5a
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Displacement of [125 I] ET-1 from rat aortic smooth muscle A7r5 cells transfected with Endothelin A receptor
Displacement of [125 I] ET-1 from rat aortic smooth muscle A7r5 cells transfected with Endothelin A receptor
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[PMID: 10969989] |
| HepG2 | IC50 |
2622 μM
Compound: 1a, SMX
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Cytotoxicity against human HepG2 cells after 24 hrs by MTS assay
Cytotoxicity against human HepG2 cells after 24 hrs by MTS assay
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[PMID: 22365879] |
| MT4 | ED50 |
>79 μM
Compound: A047
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Effective dose of compound required to inhibit replication of human immunodeficiency virus type 1 in MT-4 cells
Effective dose of compound required to inhibit replication of human immunodeficiency virus type 1 in MT-4 cells
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[PMID: 15537350] |
In Vitro
Sulfamethoxazole (0.01-1.28 mg/mL; 120 h) inhibits P. lutzii mitochondrial activity[1].
Sulfamethoxazole (0.01 mg/mL; 1-2 h) acts in P. lutzii as a competitor for amino acid, nucleic acids and folate cofactor biosynthesis, disrupting mitochondrial functions[1].
Sulfamethoxazole at different concentrations, by using the whole-cell patch clamp recording method, inhibits the KATP channel currents in mouse pancreatic β cells in a dose-dependent manner, and the IC50 is 0.46 mM[3].
Sulfamethoxazole at different concentrations, through the static incubation assay of islets, stimulates insulin secretion in mouse islets. It can stimulate insulin secretion at a low concentration (0.1 mM) under high-glucose (10 mM) conditions and only at a high concentration (10 mM) under low-glucose (2 mM) conditions[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:P. lutzii yeast cells
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Concentration:1.28 mg/mL, 0.64 mg/mL, 0.32 mg/mL, 0.16 mg/mL, 0.08 mg/mL, 0.04 mg/mL, 0.02 mg/mL, and 0.01 mg/mL
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Incubation Time:120 h
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Result:Produced a marked dose-dependent adverse effect on P. lutzii.
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Cell Line:P. lutzii yeast cells
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Concentration:0.01 mg/mL
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Incubation Time:1 or 2 h
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Result:The up-regulated genes included those for HSP70, HSP60, and HSP30.
The down-regulated genes included betaine aldehyde dehydrogenase (BADH), carnitine/acyl carnitine carrier (ACUH), guanine nucleotide binding protein α-1 subunit (GPA2), C6 transcription factor (CTF1B) and pleckstrin homology domain (PH).
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 723-46-6
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Appearance Solid
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Molecular Weight 253.28
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Formula C10H11N3O3S
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Color White to off-white
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SMILES
O=S(NC1=NOC(C)=C1)(C2=CC=C(C=C2)N)=O
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Synonyms
Ro 4-2130 1000 µg/mL in methanol
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (24)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
NAD+ exhaustion by CD38 upregulation contributes to blood pressure elevation and vascular damage in hypertension. [Abstract]2023 Sep 18;8(1):353. PMID: 37718359 -
Autophagy
2023 Oct;19(10):2702-2718. PMID: 37312409 -
Water Res
Quantifying community-wide antibiotic usage via urban water fingerprinting: Focus on contrasting resource settings in South Africa. [Abstract]2023 Jul 15:240:120110. PMID: 37247434 -
J Exp Med
2026 Mar 2;223(3):e20241287. PMID: 41400657 -
ACS Environ Au
Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]2025 Aug 5;5(6):573-582. PMID: 41277996 -
Food Chem X
Machine learning prioritization of antibiotic residues in aquatic foods reveals exposure-driven genotoxic risk mediated by BCL2. [Abstract]2026 Feb 8:34:103646. PMID: 41756597 -
Clin Chem
Discovering Cross-Reactivity in Urine Drug Screening Immunoassays through Large-Scale Analysis of Electronic Health Records. [Abstract]2019 Dec;65(12):1522-1531. PMID: 31578215 -
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Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
Pharmaceutics
Plasma Protein Binding, Biostability, Metabolite Profiling, and CYP450 Phenotype of TPB15 Across Different Species: A Novel Smoothened Inhibitor for TNBC Therapy. [Abstract]2025 Mar 26;17(4):423. PMID: 40284418 -
Virulence
Antibacterial efficacy and mechanism of the novel antimicrobial peptide lachnospirin-1 against Acinetobacter baumannii. [Abstract]2026 Dec 31;17(1):2646808. PMID: 41838520 -
Ecotoxicol Environ Saf
Microbial profiles and immune responses in seahorse gut and brood pouch under chronic exposure to environmental antibiotics. [Abstract]2023 Mar 15:253:114711. PMID: 36868035
Sulfamethoxazole 1000 µg/mL in methanol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]
Heat map of several key genes involved in immunity, metabolism, and circadian rhythm within the gut of juvenile and adult seahorses treated with Sulfamethoxazole (SMX) (1 μg/L; 4 weeks) was constructed using Tbtools software.
Sulfamethoxazole 1000 µg/mL in methanol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]
Sulfamethoxazole (SMX) (1 μg/L; 4 weeks) increased the relative abundance of Proteobacteria, whereas decreased the relative abundance of Actinobacteria and Acidobacteria.
Sulfamethoxazole 1000 µg/mL in methanol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]
Sulfamethoxazole (SMX) (1 μg/L; 4 weeks) significantly increased the ACE index, but significantly reduced the Simpson's index.
Sulfamethoxazole 1000 µg/mL in methanol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]
Sulfamethoxazole (SMX) (1 μg/L; 4 weeks) considerably increased the relative abundances of potential pathogens.
Sulfamethoxazole 1000 µg/mL in methanol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]
Sulfamethoxazole (SMX) (1 μg/L; 4 weeks). Heat map of some of representative genes related to immunity and drug metabolism in seahorse brood pouches.
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Antibiotics (Basel)
Revitalizing Trimethoprim/Sulfamethoxazole via Nanotechnology for Improved Pharmacokinetics and Antibacterial Efficacy. [Abstract]2026 Mar 10;15(3):283. PMID: 41892445 -
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Microorganisms
Isolation, Antimicrobial Susceptibility, and Genotypes of Three Pasteurellaeae Species Prevalent on Pig Farms in China Between 2021 and 2023. [Abstract]2025 Apr 18;13(4):938. PMID: 40284774 -
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Eur J Clin Microbiol Infect Dis
Evaluation of EUCAST rapid antimicrobial susceptibility testing (RAST) for carbapenemase-producing Klebsiella pneumoniae with focus on KPC variants. [Abstract]2025 Sep 29. PMID: 41023358 -
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Chemosphere
Mass-balance-model-based evaluation of sewage treatment plant contribution to residual pharmaceuticals in environmental waters. [Abstract]2019 Jun:225:378-387. PMID: 30884299
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Bacterial live/dead nucleic-acid viability staining
The LIVE/DEAD bacterial viability staining method is based on differential permeability of nucleic-acid-binding fluorescent dyes, most commonly SYTO 9 and propidium iodide (PI), which enables discrimination of bacterial populations with intact versus compromised cytoplasmic membranes. SYTO 9 penetrates both intact and damaged bacterial membranes and binds nucleic acids to produce green fluorescence, whereas propidium iodide penetrates only cells with compromised membranes and fluoresces red while also reducing SYTO 9 signal through competitive binding and fluorescence interactions. The resulting fluorescence pattern is interpreted as a proxy for membrane integrity, which is widely used as an indicator of bacterial viability in microscopy, flow cytometry, and spectroscopic platforms. However, mechanistic studies show that SYTO 9 and PI interactions involve displacement and fluorescence resonance energy transfer effects, which can influence signal interpretation depending on dye ratios a
Purity & Documentation
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Data Sheet (275 KB)
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SDS (563 KB)
- English - EN (563 KB)
- Français - FR (563 KB)
- Deutsch - DE (563 KB)
- Norwegian - NO (563 KB)
- Español - ES (563 KB)
- Swedish - SV (563 KB)
- Italian - IT (563 KB)
- Korean - KR (563 KB)
- Portuguese - PT (563 KB)
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Handling Instructions (2659 KB)
References
[1]. Zambuzzi-Carvalho et al. Transcriptional profile of the human pathogenic fungus Paracoccidioides lutzii in response to sulfamethoxazole. Med Mycol. 2015;53(5):477-492. [Content Brief]
[2]. Choonara BF, et al. A menthol-based solid dispersion technique for enhanced solubility and dissolution of sulfamethoxazole from an oral tablet matrix. AAPS PharmSciTech. 2015 Aug;16(4):771-86. [Content Brief]
[3]. Ogata H, et al. Dose Dependent Effect of Sulfamethoxazole on Inhibiting KATP Channel of Mouse Pancreatic β Cell. Dose Response. 2023 Sep 28;21(3):15593258231203611. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Sulfamethoxazole 1000 µg/mL in methanol Related Classifications
HY-B0322B Related Classifications
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