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Cat. No.: HY-N21964

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Sutherlandioside D

Sutherlandioside D is a natural product present in the aerial parts of Sutherlandia frutescens. Sutherlandioside D acts as an inhibitor of the Gli/hedgehog signaling pathway and exhibits reactive oxygen species (ROS) regulatory activity. Sutherlandioside D inhibits Gli reporter gene activity and reduces ROS production. Sutherlandioside D can be used in prostate cancer-related research.

For research use only. We do not sell to patients.

CAS No.: 1055329-49-1
Formula: C₃₆H₅₈O₉
Molecular Weight:634.84

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Sutherlandioside D Chemical Structure

CAS No.: 1055329-49-1

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Protocols (1)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

All Endogenous Metabolite Isoforms :

In Vitro

Sutherlandioside D (0.1-10 μg/mL; 48 h) potently inhibits Gli-reporter activity in stimulated Shh Light II cells with an IC₅₀ of 1.8 μg/mL^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS No. 1055329-49-1
Molecular Weight 634.84
Formula C₃₆H₅₈O₉
SMILES

O=C1[C@]23[C@@]4(C3)[C@]([C@H](C[C@@]2([H])C(C)(C=C1)C)O)([H])[C@]5([C@](CC4)([C@@](CC5)([H])[C@H](C)CC[C@H](O)C(C)(C)O[C@@H]6O[C@@H]([C@H]([C@@H]([C@H]6O)O)O)CO)C)C
Structure Classification
- Saccharides
- Monosaccharides
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Avula B, et al. Quantitative determination of flavonoids and cycloartanol glycosides from aerial parts of Sutherlandia frutescens (L.) R. BR. by using LC-UV/ELSD methods and confirmation by using LC-MS method. J Pharm Biomed Anal. 2010 Jun 5;52(2):173-80.

[2]. Lin H, et al. Inhibition of Gli/hedgehog signaling in prostate cancer cells by "cancer bush" Sutherlandia frutescens extract. Cell biology international. 2016 Feb;40(2):131-42. [Content Brief]

[3]. Lei W, et al. Unveiling the anti-inflammatory activity of Sutherlandia frutescens using murine macrophages. International immunopharmacology. 2015 Dec;29(2):254-262.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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