Tat-βsyn-degron
Tat-βsyn-degron is a Degron peptide that can cross the blood-brain barrier. Tat-βsyn-degron binds to α-synuclein through a β-synuclein-derived domain, promotes proteasomal degradation, and can induce the release of lactate dehydrogenase from primary cortical neurons. Tat-βsyn-degron can be used in research on Parkinson's disease.
For research use only. We do not sell to patients.
- CAS No.: 2816095-52-8
- Formula: C132H237N59O31
- Molecular Weight:3146.67
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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All α-synuclein Isoforms
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Biological Activity
Description
In Vitro
Tat-βsyn-degron (25 µM; 48 h) reduces α-synuclein and protects TH-positive dopaminergic neurons in rat ventral midbrain cultures exposed to 20 µM MPP+[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Primary rat cortical neurons
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Concentration:5; 12.5; 25; 50 μM
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Incubation Time:24 h
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Result:Reduced the expression levels of endogenous α-synuclein in primary rat cortical neurons.
The inhibitory effect on α-synuclein was blocked by MG132.
In Vivo
Tat-βsyn-degron (6 µmol/kg; intraperitoneal injection; twice daily; 12 days) protects dopaminergic neurons and motor function in MPTP-treated mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:M83 hemizygous transgenic mice overexpressing human A53T α-synuclein (male, 12 weeks old, 25-30 g)[1]
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Dosage:40 mg/kg
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Administration:i.p.; single injection
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Result:Brain α-synuclein levels were reduced at 12 and 24 h after injection but not at 48 h.
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Animal Model:M83 hemizygous transgenic mice overexpressing human A53T α-synuclein (male, 12 weeks old, 25-30 g)[1]
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Dosage:40 mg/kg
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Administration:i.p.; daily for 12 days, then every other day for 8 days; 20 days total
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Result:Phosphorylated S129 α-synuclein immunostaining was reduced in the substantia nigra pars compacta and pons.
Iba-1 staining was reduced in the pons, with no observable effect in the substantia nigra pars compacta.
No obvious PD-related behavioral phenotypes were observed in either group at 45 days or 3 months.
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Animal Model:C57BL/6 (male, 20-25 g)[1]
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Dosage:6 µmol/kg
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Administration:i.p.; twice daily; 12 days
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Result:Reduced α-synuclein in the substantia nigra-containing ventral midbrain and striatum.
Protected against MPTP-induced loss of TH protein in both ventral midbrain and striatum, protected TH-positive neurons in the substantia nigra pars compacta and TH-positive terminals in the striatum, and rescued MPTP-induced motor deficits in the rotarod test.
Chemical Information
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CAS No. 2816095-52-8
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Molecular Weight 3146.67
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Formula C132H237N59O31
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Sequence
Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Arg-Thr-Lys-Ser-Gly-Val-Tyr-Leu-Val-Gly-Arg-Arg-Arg-Gly
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Sequence Shortening
YGRKKRRQRRRRTKSGVYLVGRRRG
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Tat-βsyn-degron Related Classifications
HY-P10360 Related Classifications
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