1. Anti-infection
  2. Virus Protease
    HSV
    HIV
  3. Theaflavin 3,3'-digallate

Theaflavin 3,3'-digallate (Synonyms: TF-3; ZP10)

Cat. No.: HY-N1992 Purity: 99.73%
Handling Instructions

Theaflavin 3,3'-digallate (TF-3) is a potent Zika virus (ZIKV) protease inhibitor with an IC50 of 2.3 μM. Theaflavin 3,3'-digallat directly binds to ZIKVpro (Kd=8.86 µM) and inhibits ZIKV replication. Theaflavin 3,3'-digallat inhibits the activity of gp41 and NS2B-3 protease and has antiviral activity against HSV and HIV-1. Theaflavin 3,3'-digallate, the typical pigment in black tea, is a potent antitumor agent.

For research use only. We do not sell to patients.

Theaflavin 3,3'-digallate Chemical Structure

Theaflavin 3,3'-digallate Chemical Structure

CAS No. : 30462-35-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 328 In-stock
Estimated Time of Arrival: December 31
5 mg USD 202 In-stock
Estimated Time of Arrival: December 31
10 mg USD 343 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

Theaflavin 3,3'-digallate (TF-3) is a potent Zika virus (ZIKV) protease inhibitor with an IC50 of 2.3 μM. Theaflavin 3,3'-digallat directly binds to ZIKVpro (Kd=8.86 µM) and inhibits ZIKV replication. Theaflavin 3,3'-digallat inhibits the activity of gp41 and NS2B-3 protease and has antiviral activity against HSV and HIV-1[1]. Theaflavin 3,3'-digallate, the typical pigment in black tea, is a potent antitumor agent[2].

IC50 & Target[1]

HSV

 

HIV-1

 

In Vitro

Theaflavin 3,3'-digallate (TF-3; 6.25, 12.5, 25 μM; 24 hours) markedly reduces viral RNA copy numbers and NS3, U87 MG protein expression in a dose-dependent manner[1].
Theaflavin 3,3'-digallate inhibits dose-dependently ZIKV replication in Vero E6 cells (EC50=7.65 μM). Theaflavin 3,3'-digallate has minor cytotoxicity up to 40 μM in Vero E6 cells. Theaflavin 3,3'-digallate can inhibit the post-entry events of the ZIKV replication cycle from gene transcription and translation levels[1].
Theaflavin 3,3'-digallate is generally regarded as the effective component for the inhibitory effects against carcinogenesis without adverse side effects by affecting multiple signal transduction pathways, such as upregulating p53 and p21, inhibiting phosphorylation of the cell survival protein Akt and MAPK pathway, downregulation of NF-κB, shifting the ratio between pro-/antiapoptotic proteins. Theaflavin 3,3'-digallate causes a rapid and sustained decrease in phospho-ERK1/2 and -MEK1/2 protein expression. Theaflavin 3,3'-digallate inhibits HCT116 cell growth with an IC50 of 17.26 μM[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: Vero E6 cells
Concentration: 6.25, 12.5, 25 μM
Incubation Time: 24 hours
Result: Markedly reduced NS3, U87 MG protein expression in a dose-dependent manner.
Molecular Weight

868.70

Formula

C₄₃H₃₂O₂₀

CAS No.

30462-35-2

SMILES

O=C(C1=CC(O)=C(O)C(O)=C1)O[[email protected]](CC2=C3O)[[email protected]](OC2=CC(O)=C3)C4=C(C=C([[email protected]@H](OC5=CC(O)=CC(O)=C5C6)[[email protected]@H]6OC(C7=CC(O)=C(O)C(O)=C7)=O)C=C(O)C8=O)C8=C(O)C(O)=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 41.67 mg/mL (47.97 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1511 mL 5.7557 mL 11.5115 mL
5 mM 0.2302 mL 1.1511 mL 2.3023 mL
10 mM 0.1151 mL 0.5756 mL 1.1511 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (2.39 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (2.39 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 99.73%

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Keywords:

Theaflavin 3,3'-digallateTF-3 ZP10TF3TF 3ZP10ZP 10ZP-10Virus ProteaseHSVHIVHerpes simplex virusHuman immunodeficiency virusZIKVreplicationtranslationp53p21AktMAPKNF-κBERK1/2-MEK1/2NS3U87gp41NS2B-3Inhibitorinhibitorinhibit

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Theaflavin 3,3'-digallate
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