1. Metabolic Enzyme/Protease
  2. Lipoxygenase
  3. ThioLox

ThioLox is a competitive 15-lipoxygenase-1 (15-LOX-1) inhibitor with a Ki of 3.30 μM. ThioLox shows anti-inflammatory and neuroprotective properties.

For research use only. We do not sell to patients.

ThioLox Chemical Structure

ThioLox Chemical Structure

CAS No. : 1202193-89-2

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
5 mg USD 60 In-stock
10 mg USD 100 In-stock
25 mg USD 220 In-stock
50 mg USD 370 In-stock
100 mg USD 630 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

View All Lipoxygenase Isoform Specific Products:

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

ThioLox is a competitive 15-lipoxygenase-1 (15-LOX-1) inhibitor with a Ki of 3.30 μM. ThioLox shows anti-inflammatory and neuroprotective properties[1].

IC50 & Target

Ki: 3.30 μM (15-LOX-1)[1]

In Vitro

ThioLox shows competitive inhibition thus indicating non-covalent interaction with the active site of the 15-LOX-1[1].
ThioLox (50 μM; 24 h) inhibits interleukin (IL-1β, IL-6, IL-8, IL-12b, TNFα and iNOS) expression in precision-cut lung slices (PCLS)[1].
ThioLox (10 μM; 16 h) prevent lipid peroxidation and mitochondrial superoxide formation in neuronal cells[1].
ThioLox (5-20 μM; 14-16 h) protects HT-22 cells against glutamate toxicity and significantly reduces neuronal cell death[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR[1]

Cell Line: Precision-cut lung slices (PCLS)
Concentration: 1, 5, 10, 25 and 50 μM
Incubation Time: In combination with 10 μM linoleic acid for 20 h and stimulated with 10 ng/mL LPS for the last 4 h.
Result: Provided almost 50% inhibition of the expression of the pro-inflammatory genes IL-1β, IL-6, IL-8, IL-12b, TNFα and iNOS at 50 μM.

Cell Viability Assay[1]

Cell Line: Neuronal HT-22 cells
Concentration: 5, 10 and 20 μM
Incubation Time: 14-16 h
Result: Significantly reduced neuronal cell death against glutamate toxicity.
In Vivo

ThioLox has a logP of 3 and shows values that comply very well with the BBB permeability[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

274.38

Formula

C15H18N2OS

CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=C(N)SC(C2=CC=CC=C2)=C1)NCCCC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (364.46 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6446 mL 18.2229 mL 36.4458 mL
5 mM 0.7289 mL 3.6446 mL 7.2892 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.11 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 2.5 mg/mL (9.11 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.6446 mL 18.2229 mL 36.4458 mL 91.1145 mL
5 mM 0.7289 mL 3.6446 mL 7.2892 mL 18.2229 mL
10 mM 0.3645 mL 1.8223 mL 3.6446 mL 9.1115 mL
15 mM 0.2430 mL 1.2149 mL 2.4297 mL 6.0743 mL
20 mM 0.1822 mL 0.9111 mL 1.8223 mL 4.5557 mL
25 mM 0.1458 mL 0.7289 mL 1.4578 mL 3.6446 mL
30 mM 0.1215 mL 0.6074 mL 1.2149 mL 3.0372 mL
40 mM 0.0911 mL 0.4556 mL 0.9111 mL 2.2779 mL
50 mM 0.0729 mL 0.3645 mL 0.7289 mL 1.8223 mL
60 mM 0.0607 mL 0.3037 mL 0.6074 mL 1.5186 mL
80 mM 0.0456 mL 0.2278 mL 0.4556 mL 1.1389 mL
100 mM 0.0364 mL 0.1822 mL 0.3645 mL 0.9111 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
ThioLox
Cat. No.:
HY-139196
Quantity:
MCE Japan Authorized Agent: