VRC-01
Based on 1 Customer Validation
VRC-01 is a broadly neutralizing IgG1 monoclonal antibody that targets HIV-1 envelope glycoprotein gp120 Protein. VRC-01 blocks HIV-1 viral entry by mimicking CD4 receptor interaction with HIV-1 gp120 and neutralizes broad HIV-1 clades. VRC-01 can be used for the research of HIV-1 infection.
For research use only. We do not sell to patients.
- Purity : 99.805%
- CAS No.: 1412901-55-3
- Molecular Weight:145.87 kDa
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Storage:Shipping with dry ice
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
[1]|
HIV-1 |
In Vitro
VRC-01 binds HIV-1 gp120 IIIB with Kd values of 1.68 nM and 1.39 nM for transient and stable tobacco plant-produced VRC-01, respectively[2].
VRC-01 (1-24 h) from transient and stable tobacco plants neutralize HIV-1 BaL in TZM-bl cells with IC50 values of 0.339 μg/mL and 0.407 μg/mL, respectively[2].
VRC-01 (1-48 h) from transient tobacco plants neutralizes a panel of 10 HIV-1 Env pseudoviruses (clades A, B, C, G), while stable plant-produced VRC-01 shows slightly reduced potency against the same panel[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | 2011-05 | PHASE1 |
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
ELISA, FACS, Functional assay
Verified Bioactivity
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Immobilized envelope glycoprotein gp120 Protein, HIV-1 (AAC31819, HEK293, His, HY-P76390) can bind VRC-01. The EC50 for this effect is 6.987 ng/mL.
Chemical Information
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CAS No. 1412901-55-3
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Appearance Liquid
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Molecular Weight 145.87 kDa
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Color Colorless to light yellow
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SMILES
[VRC-01]
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (260 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Boesch AW, et al. Highly parallel characterization of IgG Fc binding interactions. MAbs. 2014;6(4):915-927. [Content Brief]
[2]. Teh AY, et al. Characterization of VRC01, a potent and broadly neutralizing anti-HIV mAb, produced in transiently and stably transformed tobacco. Plant Biotechnol J. 2014;12(3):300-311. [Content Brief]
[3]. Sullivan MA, et al. Anti-idiotypic monobodies derived from a fibronectin scaffold. Biochemistry. 2013;52(10):1802-1813. [Content Brief]
[4]. Stelter S, et al. Engineering the interactions between a plant-produced HIV antibody and human Fc receptors. Plant Biotechnol J. 2020;18(2):402-414. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
VRC-01 Related Classifications
HY-P991069 Related Classifications
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