Asperuloside
Based on 3 publication(s) in Google Scholar
Asperuloside is an iridoid isolated from Hedyotis diffusa, with anti-inflammatory activity. Asperuloside inhibits inducible nitric oxide synthase (iNOS), suppresses NF-κB and MAPK signaling pathways.
For research use only. We do not sell to patients.
- Purity : 99.56%
- CAS No.: 14259-45-1
- Formula: C18H22O11
- Molecular Weight:414.36
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Asperuloside
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Biological Activity
Description
IC50 & Target
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iNOS |
In Vitro
Asperuloside (0-160 μg/mL, 1 h) inhibits productions of NO, PGE2, TNF-α and IL-6 in LPS-Induced RAW 264.7 cells[1].
Asperuloside (0-5 mM, 24 h) inhibits cell viability and induces ER stress dependent apoptosis in leukemia cell lines (U937, HL-60, AML)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:U937 cells
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Concentration:0-5 mM
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Incubation Time:24 h
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Result:Increased the cleaved Caspase-3, Caspase-9, and PARP, and Cyto-c release from mitochondria.
Induced expression of GRP78, p-PERK, p-eIF2α, CHOP, p-IRE1, XBP1, ATF6 and cleaved Caspase-12
In Vivo
Asperuloside (30 and 60 mg/kg, i.p., 30 days) inhibits tumor growth of U937 xenografts mice model[3].
Asperuloside (20-80 mg/kg, i.p.) relieves LPS-induced acute lung injury via inhibiting MAPK and NF-κB signaling in BALB/c mice[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rats consuming a high fat diet (HFD)[2]
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Dosage:3 mg/day
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Administration:p.o., 0.3% in diet, daily, 12 weeks
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Result:Reduced body weight, energy intake, adiposity, blood glucose, and plasma insulin.
Chemical Information
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CAS No. 14259-45-1
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Appearance Solid
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Molecular Weight 414.36
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Formula C18H22O11
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Color Off-white to light yellow
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SMILES
O=C1O[C@]2([H])[C@@]3([H])[C@](C(COC(C)=O)=C2)([H])[C@H](O[C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O)O)O)OC=C13
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (3)
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Journal Impact Factor
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Most Recent
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J Ethnopharmacol
Ginseng root extract attenuates inflammation by inhibiting the MAPK/NF-κB signaling pathway and activating autophagy and p62-Nrf2-Keap1 signaling in vitro and in vivo. [Abstract]2022 Jan 30:283:114739. PMID: 34648903 -
Molecules
2023 Mar 22;28(6):2867. PMID: 36985838
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (241.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 25 mg/mL (60.33 mM; Need ultrasonic)
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (285 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. He J, et al. Asperuloside and Asperulosidic Acid Exert an Anti-Inflammatory Effect via Suppression of the NF-κB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Macrophages. Int J Mol Sci. 2018 Jul 12;19(7). [Content Brief]
[2]. Ishaq M, et al. Asperuloside Enhances Taste Perception and Prevents Weight Gain in High-Fat Fed Mice. Front Endocrinol (Lausanne). 2021 Apr 13;12:615446. [Content Brief]
[3]. Rong C, et al. Asperuloside exhibits a novel anti-leukemic activity by triggering ER stress-regulated apoptosis via targeting GRP78. Biomed Pharmacother. 2020 May;125:109819. [Content Brief]
[4]. Qiu J, et al. Pretreatment with the compound asperuloside decreases acute lung injury via inhibiting MAPK and NF-κB signaling in a murine model. Int Immunopharmacol. 2016 Feb;31:109-15. [Content Brief]
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 2.4134 mL | 12.0668 mL | 24.1336 mL | 60.3340 mL |
| 5 mM | 0.4827 mL | 2.4134 mL | 4.8267 mL | 12.0668 mL | |
| 10 mM | 0.2413 mL | 1.2067 mL | 2.4134 mL | 6.0334 mL | |
| 15 mM | 0.1609 mL | 0.8045 mL | 1.6089 mL | 4.0223 mL | |
| 20 mM | 0.1207 mL | 0.6033 mL | 1.2067 mL | 3.0167 mL | |
| 25 mM | 0.0965 mL | 0.4827 mL | 0.9653 mL | 2.4134 mL | |
| 30 mM | 0.0804 mL | 0.4022 mL | 0.8045 mL | 2.0111 mL | |
| 40 mM | 0.0603 mL | 0.3017 mL | 0.6033 mL | 1.5084 mL | |
| 50 mM | 0.0483 mL | 0.2413 mL | 0.4827 mL | 1.2067 mL | |
| 60 mM | 0.0402 mL | 0.2011 mL | 0.4022 mL | 1.0056 mL | |
| DMSO | 80 mM | 0.0302 mL | 0.1508 mL | 0.3017 mL | 0.7542 mL |
| 100 mM | 0.0241 mL | 0.1207 mL | 0.2413 mL | 0.6033 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Asperuloside Related Classifications
HY-N1382 Related Classifications
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