Cat. No.: HY-N0774

Isofraxidin

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Isofraxidin, a coumarin component from Acanthopanax senticosus, inhibits MMP-7 expression and cell invasion of human hepatoma cells. Isofraxidin inhibits the phosphorylation of ERK1/2 in hepatoma cells. Isofraxidin attenuates the expression of iNOS and COX-2, Isofraxidinalso inhibits TLR4/myeloid differentiation protein-2 (MD-2) complex formation.

For research use only. We do not sell to patients.

Purity: 99.94%
CAS No.: 486-21-5
Formula: C₁₁H₁₀O₅
Molecular Weight:222.19

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Isofraxidin Chemical Structure

CAS No.: 486-21-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Isofraxidin, a coumarin component from Acanthopanax senticosus, inhibits MMP-7 expression and cell invasion of human hepatoma cells. Isofraxidin inhibits the phosphorylation of ERK1/2 in hepatoma cells^[1]. Isofraxidin attenuates the expression of iNOS and COX-2, Isofraxidinalso inhibits TLR4/myeloid differentiation protein-2 (MD-2) complex formation^[2].

IC₅₀ & Target^[1]^[2]

COX-2

TLR4

MMP-7

All ERK Isoforms :

All COX Isoforms :

All MMP Isoforms :

All Toll-like Receptor (TLR) Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.4 μg/mL Compound: Isofraxidin	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 31784199]
BV-2	IC₅₀	>100 μM Compound: 12	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay	[PMID: 27919656]
HCT-15	IC₅₀	0.4 μg/mL Compound: Isofraxidin	Cytotoxicity against human HCT15 cells by SRB assay Cytotoxicity against human HCT15 cells by SRB assay	[PMID: 31784199]
KB	ED₅₀	>100 μg/mL Compound: 1, isofraxidin, NSC-324637	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 7463096]
P388	ED₅₀	1.7 μg/mL Compound: 1, isofraxidin, NSC-324637	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 7463096]
RAW264.7	IC₅₀	>100 μM Compound: 14	Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay	[PMID: 25666824]
SK-MEL-2	IC₅₀	0.4 μg/mL Compound: Isofraxidin	Cytotoxicity against human SK-MEL-2 cells by SRB assay Cytotoxicity against human SK-MEL-2 cells by SRB assay	[PMID: 31784199]
SK-OV-3	IC₅₀	0.4 μg/mL Compound: Isofraxidin	Cytotoxicity against human SKOV3 cells by SRB assay Cytotoxicity against human SKOV3 cells by SRB assay	[PMID: 31784199]
XF498	IC₅₀	0.4 μg/mL Compound: Isofraxidin	Cytotoxicity against human XF498 cells by SRB assay Cytotoxicity against human XF498 cells by SRB assay	[PMID: 31784199]

In Vitro

Isofraxidin inhibits expression of MMP-7 and in vitro cell invasion at a non-toxic level through inhibiting ERK1/2 phosphorylation in hepatoma cell lines^[1].
Isofraxidin competitively inhibits TLR4/MD-2 complex formation, and thus TLR4/NF-κB signalling cascades.Isofraxidin has potential in the treatment of Osteoarthritis (OA)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 486-21-5
Appearance Solid
Molecular Weight 222.19
Formula C₁₁H₁₀O₅
Color Off-white to yellow
SMILES

O=C1C=CC2=CC(OC)=C(O)C(OC)=C2O1
Structure Classification
- Phenylpropanoids
- Coumarins
- Phenols
- Monophenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (4)

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Journal Impact Factor

Acupunct Herb Med

2024 Nov 12.
J Ethnopharmacol

Sarcandra glabra (Thunb.) Nakai relieves pain behavior by inhibiting NaV1.7 channels. [Abstract]

2025 May 22:120002. PMID: 40412780
Int Immunopharmacol

The coumarin component isofraxidin targets the G-protein-coupled receptor S1PR1 to modulate IL-17 signaling and alleviate ulcerative colitis. [Abstract]

2024 Apr 20:131:111814. PMID: 38479159
J Cent Nerv Syst Dis

Neuroprotective potential of isofraxidin: Alleviating parkinsonian symptoms, inflammation and microglial activation. [Abstract]

2025 Jan 9:17:11795735241312661. PMID: 39790641

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (1125.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.5007 mL

22.5033 mL

45.0065 mL

5 mM

0.9001 mL

4.5007 mL

9.0013 mL

10 mM

0.4501 mL

2.2503 mL

4.5007 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (9.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (9.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yamazaki T, et al. Isofraxidin, a coumarin component from Acanthopanax senticosus, inhibits matrix metalloproteinase-7 expression and cell invasion of human hepatoma cells.Biol Pharm Bull. 2010;33(10):1716-22. [Content Brief]

[2]. Jin J , et al. Isofraxidin targets the TLR4/MD-2 axis to prevent osteoarthritis development.Food Funct. 2018 Nov 14;9(11):5641-5652. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.5007 mL	22.5033 mL	45.0065 mL	112.5163 mL
	5 mM	0.9001 mL	4.5007 mL	9.0013 mL	22.5033 mL
	10 mM	0.4501 mL	2.2503 mL	4.5007 mL	11.2516 mL
	15 mM	0.3000 mL	1.5002 mL	3.0004 mL	7.5011 mL
	20 mM	0.2250 mL	1.1252 mL	2.2503 mL	5.6258 mL
	25 mM	0.1800 mL	0.9001 mL	1.8003 mL	4.5007 mL
	30 mM	0.1500 mL	0.7501 mL	1.5002 mL	3.7505 mL
	40 mM	0.1125 mL	0.5626 mL	1.1252 mL	2.8129 mL
	50 mM	0.0900 mL	0.4501 mL	0.9001 mL	2.2503 mL
	60 mM	0.0750 mL	0.3751 mL	0.7501 mL	1.8753 mL
	80 mM	0.0563 mL	0.2813 mL	0.5626 mL	1.4065 mL
	100 mM	0.0450 mL	0.2250 mL	0.4501 mL	1.1252 mL

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Isofraxidin

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Publications Citing Use of MedChemExpress (MCE) Isofraxidin

All ERK Isoforms

All COX Isoforms

All MMP Isoforms

All Toll-like Receptor (TLR) Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Isofraxidin Related Classifications

HY-N0774 Related Classifications