Methoxy-X04
Based on 6 publication(s) in Google Scholar
Methoxy-X04 is a fluorescent dye that crosses the blood-brain barrier and selectively binds to beta-pleated sheets found in dense core amyloid Aβ plaques. Methoxy-X04 retains in vitro binding affinity for amyloid b (Ab) fibrils (Ki= 26.8 nM). Methoxy-X04 is fluorescent and stains plaques, tangles, and cerebrovascular amyloid in postmortem sections of AD brain with good specificity.
For research use only. We do not sell to patients.
- Purity: 98.65%
- CAS No.: 863918-78-9
- Formula: C23H20O3
- Molecular Weight:344.40
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Methoxy-X04
More
Customer Validation & Images
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Histological Imaging/Staining
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Bio/Physico-chemical Assay
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In Vivo Imaging
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In Vivo Imaging
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Histological Imaging/Staining
Biological Activity
1. Methoxy-X04 Solution Preparation[1]
Prepare a 5 mg/mL solution of Methoxy-X04 by dissolving Methoxy-X04 into a solution containing 10% dimethyl sulfoxide (DMSO), 45% propylene glycol, and 45% sodium phosphate buffered saline (0.9% NaCl in 100 mM phosphate buffer, pH 7.4).
2. Methoxy-X04 Solution Injection
24 hr prior to perfusion, weigh the mice and give each mouse an intraperitoneal injection of methoxy-X04 at a dose of 10 mg/kg of body weight.
3. Transcardial Perfusion of the Injected Mice
4. Brain Sectioning Using a Vibratome
5. Section Screening for the Presence of Methoxy-X04-stained Plaques
Examine each section successively, to avoid drying out the sections, using the following procedure:
Add a droplet of PBS to a microscope slide with a disposable pipette, and place the section on the droplet.
Examine the section under a fluorescent microscope (excitation 340-380 nm) to identify regions containing Methoxy-X04 labeled Aβ plaques.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 863918-78-9
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Appearance Solid
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Molecular Weight 344.40
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Formula C23H20O3
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Color Light yellow to light brown
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SMILES
COC1=CC(/C=C/C2=CC=C(O)C=C2)=CC=C1/C=C/C3=CC=C(O)C=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (6)
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Journal Impact Factor
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Most Recent
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Immunity
Astrocytic calcium-dependent enzyme PAD2 governs microglia activity to exacerbate amyloid pathology via citrullinated vimentin. [Abstract]2026 Jun 9;59(6):1579-1598.e9. PMID: 41962542 -
Methoxy-X04 purchased from MedChemExpress. Usage Cited in: Matter. 2025 May 07.
Histological comparison of stained adjacent brain slices from AD mice with Methoxy-X04 (green) and SiR-Aβ (red).
Methoxy-X04 purchased from MedChemExpress. Usage Cited in: Matter. 2025 May 07.
Comparison of the permeability of SiR-Aβ and Methoxy-X04 in the in vitro BBB model.
Methoxy-X04 purchased from MedChemExpress. Usage Cited in: Matter. 2025 May 07.
Co-incubation of SiR-Aβ and Methoxy-X04 for in vivo imaging. Fluorescence images were acquired using excitation wavelengths of 488 nm for Methoxy-X04 and 635 nm for SiR-Aβ.
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Biomaterials
Engineered peripheral CD4 T cells delivery across the BBB promote intracerebral Treg conversion unleashes microglial phagocytotic activity for Alzheimer's disease treatment. [Abstract]2026 Feb:325:123574. PMID: 40763582 -
Alzheimers Dement
ACLY regulates autolysosome acidification through tubulin acetylation-mediated assembly of V-ATPase subunits in Alzheimer's disease model mice. [Abstract]2025 Nov;21(11):e70919. PMID: 41255266 -
Alzheimers Dement
Inhibition of IFITM3 in cerebrovascular endothelium alleviates Alzheimer's-related phenotypes. [Abstract]2025 Feb;21(2):e14543. PMID: 39807629
Methoxy-X04 purchased from MedChemExpress. Usage Cited in: Alzheimers Dement. 2025 Feb;21(2):e14543. [Abstract]
Live two‐photon imaging showed fluorescent probes Methoxy‐x04 (10 mg/kg) of Aβ plaques (blue) were inside the cerebral blood vessels (green).
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Methoxy-X04 purchased from MedChemExpress. Usage Cited in: RSC Adv. 2017 Aug 16.
An illustrative diagram indicating the time duration of treatment and detectable Ab deposits stain. The worms were then stained with Methoxy-X04 (1 mM in 10 mM Tris, pH 8.0) for 4 h at room temperature.
Solvent & Solubility
DMSO : 25 mg/mL (72.59 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Bisht K, et al. Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques. J Vis Exp. 2016 Jun 1;(112):54060. [Content Brief]
[2]. Klunk WE, et al. Imaging Abeta plaques in living transgenic mice with multiphoton microscopy and methoxy-X04, a systemically administered Congo red derivative. J Neuropathol Exp Neurol. 2002 Sep;61(9):797-805. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.9036 mL | 14.5180 mL | 29.0360 mL | 72.5900 mL |
| 5 mM | 0.5807 mL | 2.9036 mL | 5.8072 mL | 14.5180 mL | |
| 10 mM | 0.2904 mL | 1.4518 mL | 2.9036 mL | 7.2590 mL | |
| 15 mM | 0.1936 mL | 0.9679 mL | 1.9357 mL | 4.8393 mL | |
| 20 mM | 0.1452 mL | 0.7259 mL | 1.4518 mL | 3.6295 mL | |
| 25 mM | 0.1161 mL | 0.5807 mL | 1.1614 mL | 2.9036 mL | |
| 30 mM | 0.0968 mL | 0.4839 mL | 0.9679 mL | 2.4197 mL | |
| 40 mM | 0.0726 mL | 0.3630 mL | 0.7259 mL | 1.8148 mL | |
| 50 mM | 0.0581 mL | 0.2904 mL | 0.5807 mL | 1.4518 mL | |
| 60 mM | 0.0484 mL | 0.2420 mL | 0.4839 mL | 1.2098 mL |
Methoxy-X04 Related Classifications
HY-103240 Related Classifications
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