VB124

Name: VB124
Brand: MedChemExpress
SKU: HY-139665
Rating: 5.0 (7 reviews)

Cat. No.: HY-139665 Purity: 98.91%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

VB124 is an orally active, potent, and selective MCT4 inhibitor. VB124 can specifically inhibit lactate efflux with IC₅₀s of 8.6 nM and 19 nM for lactate import and export in MDA-MB-231 cells, respectively. VB124 is highly selective for MCT4 over MCT1. VB124 can be used for the research of cardiac hypertrophy, heart failure, and metabolism.

For research use only. We do not sell to patients.

CAS No. : 2230186-18-0

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 7 publication(s) in Google Scholar

7 Publications Citing Use of MCE VB124

•Res Sq. 2025 May 08.

Cell Proliferation/Viability Assay

Flow Cytometry

In Vivo Efficacy Study

: VB124 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Mar 18:102038. [Abstract]; Dose-response curves of the MCT4 inhibitor VB124 in HuH7 KO and Hepa1-6 KO cells.

: VB124 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Mar 18:102038. [Abstract]; Colony formation assays of HuH7 KO and Hepa1-6 KO cells treated with VB124 (10 μM).

: VB124 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Mar 18:102038. [Abstract]; KD of LDHA and treatment with the MCT1 inhibitor AR-C155858 and the MCT4 inhibitor VB124 (1, 10, 20 nM) significantly reduced the population of Foxp-3⁺ Treg cells.

: VB124 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Mar 18:102038. [Abstract]; Tumorigenicity assay of Hepa1-6 KO cells in immunoproficient mice treated with anti-PD-1 antibody and VB124 (30 mg/kg, p.o.).

: VB124 purchased from MedChemExpress. Usage Cited in: Chin Med. 2025 Oct 23;20(1):180. [Abstract]; Representative immunoblot demonstrating β-MHC and ANP protein expression in H9C2 cells treated with VB124

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MCT1 MCT2 MCT4

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

MCT4

In Vitro

VB124 (10 μM) inhibits the cell proliferation of MDA-MB-231 cells, and the cell proliferation rate is less than 50%^[1].
VB124 is highly selective for MCT4 over MCT1, showing very little MCT1 inhibitory activity (lactate export IC₅₀=24 μM) in MCT1-expressing BT20 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

VB124 (30 mg/kg; p.o.; daily for 28 days) attenuates isoproterenol-induced cardiac hypertrophy in mice^[1].
VB124 (30 mg/kg; twice per day for 180 days) has no effect on the body, heart, liver, or lung weight of mice, suggesting no overt toxicities^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	12 weeks old C57BL/6 mice^[1]
Dosage:	30 mg/kg
Administration:	Oral gavage; daily for 28 days (dissolved in 0.5% methylcellulose and 0.1% Tween-20)
Result:	Prevented cardiac hypertrophy in mice.

Molecular Weight

426.89

Formula

C₂₃H₂₃ClN₂O₄

CAS No.

2230186-18-0

Appearance

Solid

Color

White to off-white

SMILES

CC(C)(OCC1=NN(C2=CC=CC=C2Cl)C(C3=CC=CC(OC4CC4)=C3)=C1)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (234.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3425 mL	11.7126 mL	23.4252 mL
5 mM	0.4685 mL	2.3425 mL	4.6850 mL
10 mM	0.2343 mL	1.1713 mL	2.3425 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.86 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.86 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (277 KB) SDS (251 KB)

COA (254 KB) HNMR (269 KB) LCMS (106 KB)

Handling Instructions (2659 KB)

References

[1]. Cluntun AA, et.al. The pyruvate-lactate axis modulates cardiac hypertrophy and heart failure. Cell Metab. 2021 Mar 2;33(3):629-648.e10. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3425 mL	11.7126 mL	23.4252 mL	58.5631 mL
	5 mM	0.4685 mL	2.3425 mL	4.6850 mL	11.7126 mL
	10 mM	0.2343 mL	1.1713 mL	2.3425 mL	5.8563 mL
	15 mM	0.1562 mL	0.7808 mL	1.5617 mL	3.9042 mL
	20 mM	0.1171 mL	0.5856 mL	1.1713 mL	2.9282 mL
	25 mM	0.0937 mL	0.4685 mL	0.9370 mL	2.3425 mL
	30 mM	0.0781 mL	0.3904 mL	0.7808 mL	1.9521 mL
	40 mM	0.0586 mL	0.2928 mL	0.5856 mL	1.4641 mL
	50 mM	0.0469 mL	0.2343 mL	0.4685 mL	1.1713 mL
	60 mM	0.0390 mL	0.1952 mL	0.3904 mL	0.9761 mL
	80 mM	0.0293 mL	0.1464 mL	0.2928 mL	0.7320 mL
	100 mM	0.0234 mL	0.1171 mL	0.2343 mL	0.5856 mL

VB124 Related Classifications

Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

VB1242230186-18-0VB 124VB-124Monocarboxylate TransporterMDA-MB-231selectivecardiac hypertrophycelllactate effluxInhibitorinhibitorinhibit

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VB124

Customer Review

VB124 Related Antibodies

7 Publications Citing Use of MCE VB124

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Monocarboxylate Transporter Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

VB124 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

VB124 Related Classifications

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