Vem-L-Cy5
Based on 1 Customer Validation
Vem-L-Cy5 (compound 3)，modified with the NIR fluorophore cyanine-5 (Cy5)，is a Vemurafenib (HY-12057)-based inhibitor of BRAF. Vem-L-Cy5 targets to BRAFV600E，and also inhibits MEK phosphorylation. Vem-L-Cy5 has cell permeability，and inhibits cell growth of many types of cancer.
For research use only. We do not sell to patients.
- Purity: 98.31%
- CAS No.: 3057544-65-4
- Formula: C63H68F5N7O9S
- Molecular Weight:1194.31
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 8505C | IC50 |
2.5 μM
Compound: 3; Vem-L-Cy5
|
Cytotoxicity against human 8505C cells expressing homozygous BRAF V600E mutant assessed as cell growth measured after 7 days of treatment by crystal violet assay
Cytotoxicity against human 8505C cells expressing homozygous BRAF V600E mutant assessed as cell growth measured after 7 days of treatment by crystal violet assay
|
[PMID: 37182332] |
| C32 | IC50 |
0.63 μM
Compound: 3; Vem-L-Cy5
|
Cytotoxicity against human C32 cells expressing heterozygous BRAF V600E mutant assessed as cell growth measured after 7 days of treatment by crystal violet assay
Cytotoxicity against human C32 cells expressing heterozygous BRAF V600E mutant assessed as cell growth measured after 7 days of treatment by crystal violet assay
|
[PMID: 37182332] |
| HT-29 | IC50 |
0.34 μM
Compound: 3; Vem-L-Cy5
|
Cytotoxicity against human HT-29 cells expressing heterozygous BRAF V600E mutant assessed as cell growth measured after 7 days of treatment by crystal violet assay
Cytotoxicity against human HT-29 cells expressing heterozygous BRAF V600E mutant assessed as cell growth measured after 7 days of treatment by crystal violet assay
|
[PMID: 37182332] |
Chemical Information
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CAS No. 3057544-65-4
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Appearance Solid
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Molecular Weight 1194.31
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Formula C63H68F5N7O9S
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Color Light blue to blue
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SMILES
CC1(C)C(/C=C/C=C/C=C2N(CCCCCC(NCCOCCOCC(NC3=CC=C(C4=CN=C(NC=C5C(C6=C(F)C=CC(NS(=O)(CCC)=O)=C6F)=O)C5=C4)C=C3)=O)=O)C7=C(C=CC=C7)C/2(C)C)=[N+](C)C8=C1C=CC=C8.O=C([O-])C(F)(F)F
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : ≥ 50 mg/mL (41.87 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (267 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.8373 mL | 4.1865 mL | 8.3730 mL | 20.9326 mL |
| 5 mM | 0.1675 mL | 0.8373 mL | 1.6746 mL | 4.1865 mL | |
| 10 mM | 0.0837 mL | 0.4187 mL | 0.8373 mL | 2.0933 mL | |
| 15 mM | 0.0558 mL | 0.2791 mL | 0.5582 mL | 1.3955 mL | |
| 20 mM | 0.0419 mL | 0.2093 mL | 0.4187 mL | 1.0466 mL | |
| 25 mM | 0.0335 mL | 0.1675 mL | 0.3349 mL | 0.8373 mL | |
| 30 mM | 0.0279 mL | 0.1396 mL | 0.2791 mL | 0.6978 mL | |
| 40 mM | 0.0209 mL | 0.1047 mL | 0.2093 mL | 0.5233 mL |
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