2. Metabolic Enzyme/Protease
  3. Phospholipase
  4. VU533

APE-PLD (VU533) activator is a potent NAPE-PLD activator with an EC50 value of 0.30 µM. NAPE-PLD activator (VU533) can enhance NAPE-PLD activity and increase efferocytosis by macrophages. NAPE-PLD activator (VU533) can be used for cardiometabolic diseases research.

For research use only. We do not sell to patients.

VU533 Chemical Structure

VU533 Chemical Structure

CAS No. : 923417-09-8

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Based on 1 publication(s) in Google Scholar

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Description

APE-PLD (VU533) activator is a potent NAPE-PLD activator with an EC50 value of 0.30 µM. NAPE-PLD activator (VU533) can enhance NAPE-PLD activity and increase efferocytosis by macrophages. NAPE-PLD activator (VU533) can be used for cardiometabolic diseases research[1].

IC50 & Target

PLD1

0.3 μM (EC50)

In Vitro

NAPE-PLD activator (VU533) (0.1-30 μM, 24 h) has no cytotoxicity when tested at 30 μM in either RAW264.7 or HepG2 cells[1].
NAPE-PLD activator (0.1-30 μM, 24 h) increases Nape-pld activity in a concentration-dependent manner in RAW264.7 cells and HepG2 cells[1].
NAPE-PLD activator (10 μM, 6 h) enhances efferocytosis in bone-marrow derived macrophages (BMDM) [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

VU533 Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: RAW264.7, HepG2
Concentration: 0.1-30 μM
Incubation Time: 24 h
Result: Showed no cytotoxicity when tested at 30 μM in either RAW264.7 or HepG2 cells.

Cell Autophagy Assay[1]

Cell Line: BMDM
Concentration: 10 μM
Incubation Time: 6 h
Result: Enhanced efferocytosis compared to vehicle treated BMDM at 10μM.
Molecular Weight

447.55

Formula

C21H22FN3O3S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=C2SC(NC(C3CCN(S(=O)(C4=CC=C(C=C4)F)=O)CC3)=O)=NC2=C(C)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (279.30 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2344 mL 11.1719 mL 22.3439 mL
5 mM 0.4469 mL 2.2344 mL 4.4688 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2344 mL 11.1719 mL 22.3439 mL 55.8597 mL
5 mM 0.4469 mL 2.2344 mL 4.4688 mL 11.1719 mL
10 mM 0.2234 mL 1.1172 mL 2.2344 mL 5.5860 mL
15 mM 0.1490 mL 0.7448 mL 1.4896 mL 3.7240 mL
20 mM 0.1117 mL 0.5586 mL 1.1172 mL 2.7930 mL
25 mM 0.0894 mL 0.4469 mL 0.8938 mL 2.2344 mL
30 mM 0.0745 mL 0.3724 mL 0.7448 mL 1.8620 mL
40 mM 0.0559 mL 0.2793 mL 0.5586 mL 1.3965 mL
50 mM 0.0447 mL 0.2234 mL 0.4469 mL 1.1172 mL
60 mM 0.0372 mL 0.1862 mL 0.3724 mL 0.9310 mL
80 mM 0.0279 mL 0.1396 mL 0.2793 mL 0.6982 mL
100 mM 0.0223 mL 0.1117 mL 0.2234 mL 0.5586 mL
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VU533 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

VU533923417-09-8VU 533VU-533PhospholipaseNAPE-PLDsmall molecule enzyme activationefferocytosiscardiometabolic diseaseInhibitorinhibitorinhibit

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