21-Deoxycortisol
Based on 1 publication(s) in Google Scholar
21-Deoxycortisol is an adrenal-specific C21 steroid that accumulates via alternative pathways due to 21-hydroxylase (CYP21A2/P450c21) deficiency. 21-Deoxycortisol serves as a sensitive and specific biomarker for steroid 21-hydroxylase deficiency (21OHD).
For research use only. We do not sell to patients.
- Purity: 99.49%
- CAS No.: 641-77-0
- Formula: C21H30O4
- Molecular Weight:346.47
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 21-Deoxycortisol
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
21-Deoxycortisol does not increase in preterm infants or other forms of congenital adrenal hyperplasia (CAH). Quantitative detection of 21-Deoxycortisol by LC-MS/MS enables accurate differentiation of non-classical congenital adrenal hyperplasia (NCCAH), CYP21A2 heterozygotes and polycystic ovary syndrome (PCOS) in hyperandrogenemic females[1][2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 641-77-0
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Appearance Solid
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Molecular Weight 346.47
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Formula C21H30O4
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Color White to off-white
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SMILES
C[C@]1(C2)[C@](CC[C@@]1(C(C)=O)O)([H])[C@@](CC3)([H])[C@@]([C@H]2O)([H])[C@](CC4)(C)C3=CC4=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Gut Microbes
Gut microbiota signatures in primary aldosteronism and functional identification of an aldosterone-degrading gut bacterium. [Abstract]2026 Dec 31;18(1):2657047. PMID: 41964107
Solvent & Solubility
DMSO : 100 mg/mL (288.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.22 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.22 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1].
Miller WL. Congenital Adrenal Hyperplasia: Time to Replace 17OHP with 21-Deoxycortisol. Horm Res Paediatr. 2019;91(6):416-420.
[Content Brief]
[2]. Ng JL, et al. Serum 21-Deoxycortisol for Diagnosis of Nonclassic Congenital Adrenal Hyperplasia in Women With Androgen Excess. The Journal of clinical endocrinology and metabolism. 2023 Nov 17;108(12):e1560-e1570. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8863 mL | 14.4313 mL | 28.8625 mL | 72.1563 mL |
| 5 mM | 0.5773 mL | 2.8863 mL | 5.7725 mL | 14.4313 mL | |
| 10 mM | 0.2886 mL | 1.4431 mL | 2.8863 mL | 7.2156 mL | |
| 15 mM | 0.1924 mL | 0.9621 mL | 1.9242 mL | 4.8104 mL | |
| 20 mM | 0.1443 mL | 0.7216 mL | 1.4431 mL | 3.6078 mL | |
| 25 mM | 0.1155 mL | 0.5773 mL | 1.1545 mL | 2.8863 mL | |
| 30 mM | 0.0962 mL | 0.4810 mL | 0.9621 mL | 2.4052 mL | |
| 40 mM | 0.0722 mL | 0.3608 mL | 0.7216 mL | 1.8039 mL | |
| 50 mM | 0.0577 mL | 0.2886 mL | 0.5773 mL | 1.4431 mL | |
| 60 mM | 0.0481 mL | 0.2405 mL | 0.4810 mL | 1.2026 mL | |
| 80 mM | 0.0361 mL | 0.1804 mL | 0.3608 mL | 0.9020 mL | |
| 100 mM | 0.0289 mL | 0.1443 mL | 0.2886 mL | 0.7216 mL |
21-Deoxycortisol Related Classifications
HY-113405 Related Classifications
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- 21-Deoxycortisol
- 641-77-0
- Endogenous Metabolite
- steroid 21-hydroxylase deficiency
- nonclassic congenital adrenal hyperplasia
- congenital adrenal hyperplasia
- adrenal steroid
- CYP21A2 heterozygosity
- 11β-hydroxylase
- hyperandrogenic females
- 17-hydroxyprogesterone
- polycystic ovary syndrome
- premature infants
- Inhibitor
- inhibitor
- inhibit