3-Methoxy-9H-Carbazole
Based on 1 Customer Validation
3-Methoxy-9H-Carbazole is a NF-κB inhibitor. 3-Methoxy-9H-Carbazole inhibits NF-κB gene expression. 3-Methoxy-9H-Carbazole induces cell apoptosis, increases cellular caspase-3 activity and promotes ROS production. 3-Methoxy-9H-Carbazole exerts cytotoxic effects on cancer cells. 3-Methoxy-9H-Carbazole can be used in the research of breast cancer.
For research use only. We do not sell to patients.
- Purity: 99.64%
- CAS No.: 18992-85-3
- Formula: C13H11NO
- Molecular Weight:197.24
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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All Caspase Isoforms
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Biological Activity
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Caspase 3 |
3-Methoxy-9H-Carbazole (3-Methoxy carbazole) binds to the NF-κB precursor protein p105 via hydrogen bonding and hydrophobic interactions[1].
3-Methoxy-9H-Carbazole (20-80 µM; 24 h) reduces the viability of MCF-7, MDA-MB-231, and MCF-7 cells in a dose-dependent manner[1].
3-Methoxy-9H-Carbazole (20-80 µM; 24 h) dose-dependently induces apoptosis in MCF-7 cells and increases the activity of caspase-3 in the cells[1].
3-Methoxy-9H-Carbazole (20-80 µM; 12 h) dose-dependently increases intracellular ROS production in MCF-7 cells, with treatment at 80 µM elevating ROS levels by 53.75% and reducing the level of NF-κB (p105/p50) in the cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7, MDA-MB-231 cells
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Concentration:20 µM, 40 µM, 80 µM
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Incubation Time:24 h
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Result:Reduced MCF-7 cell viability in a dose-dependent manner: to 88.40% (20 µM), 62.88% (40 µM), and 34.08% (80 µM).
Induced dose-dependent cell shrinkage, swelling, and rupturing observed via phase-contrast microscopy.
Reduced MDA-MB-231 cell viability in a dose-dependent manner: to 85.33% (20 µM), 68.71% (40 µM), and 32.19% (80 µM).
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Cell Line:MCF-7 cells
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Concentration:20 µM, 40 µM, 80 µM
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Incubation Time:24 h
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Result:Induced dose-dependent apoptosis in MCF-7 cells, characterized by increased red fluorescence, nuclear chromatin condensation, and blebbing.
Showed a significant increase in apoptotic cell fluorescence relative to control at 40 µM and 80 µM.
Chemical Information
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CAS No. 18992-85-3
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Appearance Solid
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Molecular Weight 197.24
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Formula C13H11NO
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Color White to off-white
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SMILES
COC1=CC2=C(NC3=C2C=CC=C3)C=C1
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (507.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.0700 mL | 25.3498 mL | 50.6997 mL | 126.7491 mL |
| 5 mM | 1.0140 mL | 5.0700 mL | 10.1399 mL | 25.3498 mL | |
| 10 mM | 0.5070 mL | 2.5350 mL | 5.0700 mL | 12.6749 mL | |
| 15 mM | 0.3380 mL | 1.6900 mL | 3.3800 mL | 8.4499 mL | |
| 20 mM | 0.2535 mL | 1.2675 mL | 2.5350 mL | 6.3375 mL | |
| 25 mM | 0.2028 mL | 1.0140 mL | 2.0280 mL | 5.0700 mL | |
| 30 mM | 0.1690 mL | 0.8450 mL | 1.6900 mL | 4.2250 mL | |
| 40 mM | 0.1267 mL | 0.6337 mL | 1.2675 mL | 3.1687 mL | |
| 50 mM | 0.1014 mL | 0.5070 mL | 1.0140 mL | 2.5350 mL | |
| 60 mM | 0.0845 mL | 0.4225 mL | 0.8450 mL | 2.1125 mL | |
| 80 mM | 0.0634 mL | 0.3169 mL | 0.6337 mL | 1.5844 mL | |
| 100 mM | 0.0507 mL | 0.2535 mL | 0.5070 mL | 1.2675 mL |
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