5-Methoxycanthin-6-one
5-Methoxycanthin-6-one is an orally active inhibitor of Leishmania strains. 5-Methoxycanthin-6-one shows cytotoxicity with an IC50 of 5.44 μg/mL against Ailanthus altissima cells.
For research use only. We do not sell to patients.
- CAS No.: 15071-56-4
- Formula: C15H10N2O2
- Molecular Weight:250.25
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
IC50 & Target
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Leishmania |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | ED50 |
3.1 μg/mL
Compound: 6
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Cytotoxicity against human A549 cells after 3 days by SRB assay
Cytotoxicity against human A549 cells after 3 days by SRB assay
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[PMID: 11141127] |
| CAKI-1 | ED50 |
4.4 μg/mL
Compound: 6
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Cytotoxicity against human CAKI1 cells after 3 days by SRB assay
Cytotoxicity against human CAKI1 cells after 3 days by SRB assay
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[PMID: 11141127] |
| HCT-8 | ED50 |
2.5 μg/mL
Compound: 6
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Cytotoxicity against human HCT8 cells after 3 days by SRB assay
Cytotoxicity against human HCT8 cells after 3 days by SRB assay
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[PMID: 11141127] |
| KB | ED50 |
2.9 μg/mL
Compound: 6
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Cytotoxicity against human KB cells after 3 days by SRB assay
Cytotoxicity against human KB cells after 3 days by SRB assay
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[PMID: 11141127] |
| MCF7 | ED50 |
5 μg/mL
Compound: 6
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Cytotoxicity against human MCF7 cells after 3 days by SRB assay
Cytotoxicity against human MCF7 cells after 3 days by SRB assay
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[PMID: 11141127] |
| SK-MEL-2 | ED50 |
17 μg/mL
Compound: 6
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Cytotoxicity against human SK-MEL-2 cells after 3 days by SRB assay
Cytotoxicity against human SK-MEL-2 cells after 3 days by SRB assay
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[PMID: 11141127] |
Chemical Information
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CAS No. 15071-56-4
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Molecular Weight 250.25
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Formula C15H10N2O2
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SMILES
O=C1N2C3=C(N=CC=C3C4=C2C=CC=C4)C=C1OC
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
[1]. Ferreira ME, et al. Leishmanicidal activity of two canthin-6-one alkaloids, two major constituents of Zanthoxylum chiloperone var. angustifolium. J Ethnopharmacol. 2002 May;80(2-3):199-202. [Content Brief]
[2]. Anderson LA, et al. Production of cytotoxic canthin-6-one alkaloids by Ailanthus altissima plant cell cultures. J Nat Prod. 1983 May-Jun;46(3):374-8. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
5-Methoxycanthin-6-one Related Classifications
HY-N9194 Related Classifications
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