AMARA peptide TFA
Based on 1 Customer Validation
AMARA peptide TFA is a synthetic peptide substrate of sucrose non-fermenting-1-related protein kinase 1 (SnRK1). AMARA peptide TFA contains residues required for SnRK1 phosphorylation, retains the minimal motif recognized by SnRK1, and when this substrate is recognized by cauliflower SnRK1 HRK-A, the hydrophobic residues at the relative positions P-5 and P+4 of the phosphorylated serine require a critical spacing. AMARA peptide TFA can serve as a control substrate in peptide phosphorylation assays for the detection of SnRK1 activity in Arabidopsis thaliana, potato tuber tissues, and during SnRK1 purification.
For research use only. We do not sell to patients.
- Purity: 98.53%
- Formula: C64H116F3N27O18S
- Molecular Weight:1640.84
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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SnRK1 |
AMARA peptide TFA is potently phosphorylated by purified spinach SnRK1 in vitro with a specific activity of 142 nmol/min/mg[1].
AMARA peptide TFA is phosphorylated by crude soluble protein extracts from liquid-cultured Arabidopsis thaliana (Col-4) seedlings with a calcium-independent activity of 2.49 nmol/min/mg, 90% of which is attributable to SnRK1, plus a small additional calcium-dependent activity[1].
The AMARA peptide TFA functions as an improved synthetic substrate for plant SnRK1 compared to the SAMS peptide[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 1640.84
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Formula C64H116F3N27O18S
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Color White to off-white
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 50 mg/mL (30.47 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 33.33 mg/mL (20.31 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[2]. Halford NG, et al. Carbon metabolite sensing and signalling. Plant biotechnology journal. 2003 Nov;1(6):381-98. [Content Brief]
[3]. Halford NG, et al.. SNF1-related protein kinases: global regulators of carbon metabolism in plants? Plant Mol Biol. 1998 Jul;37(5):735-48. [Content Brief]
[4]. Halford NG, et al. Metabolic signalling and carbon partitioning: role of Snf1-related (SnRK1) protein kinase. Journal of experimental botany. 2003 Jan;54(382):467-75. [Content Brief]
[5]. Wu X, et al. Hydrogen sulfide promotes lateral root formation in peach through persulfidation of SnRK1α kinase. Plant Biotechnol J. 2025 Oct;23(10):4395-4411. [Content Brief]
[6]. McKibbin RS, et al. Production of high-starch, low-glucose potatoes through over-expression of the metabolic regulator SnRK1. Plant Biotechnol J. 2006 Jul;4(4):409-18. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 0.6094 mL | 3.0472 mL | 6.0944 mL | 15.2361 mL |
| 5 mM | 0.1219 mL | 0.6094 mL | 1.2189 mL | 3.0472 mL | |
| 10 mM | 0.0609 mL | 0.3047 mL | 0.6094 mL | 1.5236 mL | |
| 15 mM | 0.0406 mL | 0.2031 mL | 0.4063 mL | 1.0157 mL | |
| 20 mM | 0.0305 mL | 0.1524 mL | 0.3047 mL | 0.7618 mL | |
| 25 mM | 0.0244 mL | 0.1219 mL | 0.2438 mL | 0.6094 mL | |
| 30 mM | 0.0203 mL | 0.1016 mL | 0.2031 mL | 0.5079 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
AMARA peptide TFA Related Classifications
HY-P1576A Related Classifications
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