AZD0156

Name: AZD0156
Brand: MedChemExpress
SKU: HY-100016
Rating: 5.0 (14 reviews)

Cat. No.: HY-100016 Purity: 99.83%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

AZD0156 is a potent, selective and orally active ATM inhibitor with an IC₅₀ of 0.58 nM. AZD0156 inhibits the ATM-mediated signaling, prevents DNA damage checkpoint activation, disrupts DNA damage repair, and induces tumor cell apoptosis.

For research use only. We do not sell to patients.

CAS No. : 1821428-35-6

Size	Stock	Quantity
Free Sample (0.1 - 0.2 mg)	Apply Now
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 14 publication(s) in Google Scholar

Other Forms of AZD0156:

AZD0156 (Standard) Get quote

14 Publications Citing Use of MCE AZD0156

In Vivo Efficacy Study

Gel Electrophoresis

Cell Proliferation/Viability Assay

: AZD0156 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2025 Jun 16:189:118273. [Abstract]; Dose-response curves obtained using the MTS assay in niraparib-sensitive CCA cell lines after treatment with AZD0156 at concentrations of 5-15 μM for 72 h.

: AZD0156 purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2024 Mar;25(3):1469-1489. [Abstract]; Cell viability assays in HCT116 and HT-29 cancer cells cultured at pH 7.4 or 6.5, and treated with the indicated doses of ATMi AZD0156 (0.01-10 μM) for 72 h.

: AZD0156 purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2024 Mar;25(3):1469-1489. [Abstract]; (C, D) Effects of 10 µM ATMi AZD0156, 10 µM 5-FU or the combination of both drugs on the growth of colorectal cancer patient-derived tumor organoids. Representative pictures of organoids at day 7 post-treatment are presented (C) together with quantification (D). Scale bars = 500 µm.

: AZD0156 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Oct 9:2025.10.08.680157.; pICL^Pt was replicated in egg extract supplemented with [α-32P]dCTP and the ATM inhibitor AZD0156, as indicated. Replication intermediates were resolved on a native agarose gel. As with KU-55933 treatment, AZD0156 (100 μM; 30-240 min) treatment resulted in stabilization of reversed fork intermediates and suppressed formation of okadaic acid-induced putative fork cleavage products.

: AZD0156 purchased from MedChemExpress. Usage Cited in: PLoS One. 2022 Sep 23;17(9):e0274735. [Abstract]; Results of inducing white turbidity with galactose as in C (upper panel) and a subsequent 2–4 days in galactose medium containing AZD0156 dissolved in DMSO to final concentrations of 2.5, 5, 10, 20, and 40 μM (lower panel).

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ATM ATR TRRAP

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

ATM

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	0.46 nM Compound: AZD0156	Inhibition of colony formation in human HCT-116 cells preincubated for 1 hr followed by exposure to 2 Gy gamma irradiation and measured after 1 week by crystal violet staining based chemiluminescence assay Inhibition of colony formation in human HCT-116 cells preincubated for 1 hr followed by exposure to 2 Gy gamma irradiation and measured after 1 week by crystal violet staining based chemiluminescence assay	[PMID: 37438997]
MCF7	IC₅₀	0.6 μM Compound: 6; AZD-0156	Antiproliferative activity against human MCF7 cells measured after 2 days by SRB assay Antiproliferative activity against human MCF7 cells measured after 2 days by SRB assay	[PMID: 35231830]
SW480	IC₅₀	1.1 μM Compound: 6; AZD-0156	Antiproliferative activity against human SW480 cells measured after 3 days by SRB assay Antiproliferative activity against human SW480 cells measured after 3 days by SRB assay	[PMID: 35231830]

In Vitro

AZD0156 inhibits the kinase activity of ATM and ATM-mediated signaling, prevents DNA damage checkpoint activation, and disrupts DNA damage repair, inducs tumor cell apoptosis, and leads to cell death in ATM-overexpressing tumor cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

461.56

Formula

C₂₆H₃₁N₅O₃

CAS No.

1821428-35-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1C2CCOCC2)N(C)C3=C1C4=CC(C5=CC=C(OCCCN(C)C)N=C5)=CC=C4N=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 20 mg/mL (43.33 mM; ultrasonic and adjust pH to 3 with HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1666 mL	10.8328 mL	21.6657 mL
5 mM	0.4333 mL	2.1666 mL	4.3331 mL
10 mM	0.2167 mL	1.0833 mL	2.1666 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 0.83 mg/mL (1.80 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 0.83 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 0.83 mg/mL (1.80 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.83 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 0.83 mg/mL (1.80 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 0.83 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (273 KB) SDS (396 KB)

COA (248 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Imidazo[4,5-c]quinolin-2-one compounds and their use in treating cancer.?

Cell Assay
^[1]

HT29 cells are seeded into 384 well assay plates at a density of 6000 cells/well in 40 μL EMEM medium containing 1% L glutamine and 10% FBS and allowed to adhere overnight. The following morning compound of Formula (I) in 100% DMSO is added to assay plates by acoustic dispensing. After lh incubation at 37°C and 5% C0₂, 40 nL of 3 mM 4NQO in 100% DMSO is added to all wells by acoustic dispensing, except minimum control wells which are left untreated with 4NQO to generate a null response control. Plates are returned to the incubator for a further lh. Then cells are fixed by adding 20 μL of 3.7% formaldehyde in PBS solution and incubating for 20 mins at r.t.. Then 20 μL of 0.1% Triton XI 00 in PBS is added and incubated for 10 minutes at r.t., to permeabalise cells. Then the plates are washed once with 50 μL/well PBS, using a Biotek EL405 plate washer.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Imidazo[4,5-c]quinolin-2-one compounds and their use in treating cancer.?

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / DMSO	1 mM	2.1666 mL	10.8328 mL	21.6657 mL	54.1641 mL
	5 mM	0.4333 mL	2.1666 mL	4.3331 mL	10.8328 mL
	10 mM	0.2167 mL	1.0833 mL	2.1666 mL	5.4164 mL
	15 mM	0.1444 mL	0.7222 mL	1.4444 mL	3.6109 mL
	20 mM	0.1083 mL	0.5416 mL	1.0833 mL	2.7082 mL
	25 mM	0.0867 mL	0.4333 mL	0.8666 mL	2.1666 mL
	30 mM	0.0722 mL	0.3611 mL	0.7222 mL	1.8055 mL
	40 mM	0.0542 mL	0.2708 mL	0.5416 mL	1.3541 mL

AZD0156 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AZD01561821428-35-6AZD 0156AZD-0156ATM/ATRApoptosisAtaxia telangiectasia mutatedATM and RAD3 relatedInhibitorinhibitorinhibit

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AZD0156

Customer Review

Other Forms of AZD0156:

AZD0156 Related Antibodies

14 Publications Citing Use of MCE AZD0156

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All ATM/ATR Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

AZD0156 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

AZD0156 Related Classifications

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