1. NF-κB
    Anti-infection
    Metabolic Enzyme/Protease
    Immunology/Inflammation
  2. NF-κB
    HIV
    Reactive Oxygen Species
  3. Aurothioglucose

Aurothioglucose  (Synonyms: Gold thioglucose)

Cat. No.: HY-A0068
COA Handling Instructions

Aurothioglucose (Gold thioglucose), containing monovalent gold ion, is a potent active-site inhibitor of TrxR1 (thioredoxin reductase 1), with an IC50 of 65 nM. Aurothioglucose inhibits the DNA binding of NF-κB in vitro. Aurothioglucose shows anti-HIV and anti-rheumatic activities.

For research use only. We do not sell to patients.

Aurothioglucose Chemical Structure

Aurothioglucose Chemical Structure

CAS No. : 12192-57-3

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Based on 1 publication(s) in Google Scholar

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Description

Aurothioglucose (Gold thioglucose), containing monovalent gold ion, is a potent active-site inhibitor of TrxR1 (thioredoxin reductase 1), with an IC50 of 65 nM. Aurothioglucose inhibits the DNA binding of NF-κB in vitro. Aurothioglucose shows anti-HIV and anti-rheumatic activities[1][2].

IC50 & Target

IC50: 65 nM (TrxR1)[1]

In Vitro

Aurothioglucose (0-100 μM, 24-72 h) inhibits TrxR1 activity in HeLa cell cytosol but has no effect on the viability of the cells[1].
Aurothioglucose (0-30 μM, 6 h) exhibits very low cytotoxicity on cells[1].
Aurothioglucose (0-20 μM, 24 h) combined with Ebselen (HY-13750) shows a strong synergistic effect, leading to Trx1 (thioredoxin 1) oxidation, reactive oxygen species (ROS) accumulation, and cell death[1].
Aurothioglucose (0-100 μM, 3-12 days) inhibits p24 levels in OM10.1 and Ach2 cells, and inhibits HIV-1 replication in vitro[2].
Aurothioglucose (0-25 μM, 12 days) increases the accumulation of metal gold in a dose-dependent manner[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: HeLa cells
Concentration: 0, 5, 10, 50, 100 μM
Incubation Time: 24, 48, 72 h
Result: Inhibited TrxR activity by more than 90% at 100 μM in HeLa cells. Cell viability was unaffected by ATG treatment, even at 100 μM after 72 h.

Western Blot Analysis[1]

Cell Line: HeLa cells
Concentration: 0, 5, 10, and 100 μM
Incubation Time: 24 h
Result: Showed no significant oxidation of Trx1 or Trx2 in HeLa cells.

Western Blot Analysis[2]

Cell Line: OM10.1, Ach2 cells
Concentration: 0, 4, 10, 25 and 100 μM
Incubation Time: 3, 6 or 12 days
Result: Significantly inhibited p24 levels. After 12 days of incubation, the viability of cells treated with 10, 25 and 100 μM Aurothioglucose had decreased to 60% of the control.
In Vivo

Aurothioglucose (25 mg/kg, i.p., single) significantly attenuates lung injury and enhances survival in a clinically relevant murine model of ARDS. The protective effects of Aurothioglucose are GSH dependent[3].
Aurothioglucose (300 mg/kg, i.p., single) induces hypothalamic obesity in mice[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male C3H/HeN mice (8-12 week, LPS/hyperoxia-exposed mice, inflammation/hyperoxia ARDS model)[3]
Dosage: 25 mg/kg
Administration: IP, single, at 12 h after intratracheal LPS administration
Result: Significantly attenuated lung injury, increased lung GCLM expression and GSH levels, and decreased mortality.
Molecular Weight

392.18

Formula

C6H11AuO5S

CAS No.
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Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (318.73 mM; Need ultrasonic)

DMSO : 6 mg/mL (15.30 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5498 mL 12.7492 mL 25.4985 mL
5 mM 0.5100 mL 2.5498 mL 5.0997 mL
10 mM 0.2550 mL 1.2749 mL 2.5498 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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