1. Metabolic Enzyme/Protease
  2. Phosphatase
  3. BN82002

BN82002 

Cat. No.: HY-112776
Handling Instructions

BN82002 is a potent, selective and irreversible inhibitor of CDC25 phosphatase family. BN82002 inhibits CDC25A, CDC25B2, CDC25B3, CDC25C CDC25A, and 25C-cat with IC50 values of 2.4, 3.9, 6.3, 5.4, and 4.6 µM, respectively. BN82002 displays ~20-fold greater selectivity over CD45 tyrosine phosphatase.

For research use only. We do not sell to patients.

BN82002 Chemical Structure

BN82002 Chemical Structure

CAS No. : 396073-89-5

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Solution
10 mM * 1 mL in DMSO USD 121 Ask For Quote & Lead Time
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
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Solid
5 mg USD 110 Ask For Quote & Lead Time
10 mg USD 150 Ask For Quote & Lead Time
50 mg USD 450 Ask For Quote & Lead Time
100 mg USD 750 Ask For Quote & Lead Time

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Other Forms of BN82002:

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Description

BN82002 is a potent, selective and irreversible inhibitor of CDC25 phosphatase family. BN82002 inhibits CDC25A, CDC25B2, CDC25B3, CDC25C CDC25A, and 25C-cat with IC50 values of 2.4, 3.9, 6.3, 5.4, and 4.6 µM, respectively. BN82002 displays ~20-fold greater selectivity over CD45 tyrosine phosphatase[1].

IC50 & Target

IC50: 2.4 μM (CDC25A), 3.9 μM (CDC25B2), 6.3 μM (CDC25B3), 5.4 μM (CDC25C), 4.6 μM (CDC25C-cat)[1].

In Vitro

The effect of BN82002 on cell proliferation is evaluated in vitro on several human tumor cell lines. Menadione, which has been reported to inhibit cell proliferation, is used as a control. All of the examined cell lines are sensitive to BN82002 and Menadione in a concentration-dependent manner in the low micromolar range. The most sensitive is the pancreatic cancer cell line MIA PaCa-2 with an IC50 of 7.2 μM, and the less sensitive cell line is the colon cancer HT-29 with an IC50 of 32.6 μM. The range of activity is very similar to the one observed with menadione (5-15 μM). It is also showed that 50 μM BN82002 is a concentration that fully inhibits cell proliferation, the cell cycle distribution is only modestly affected with a slight decrease in S phase and an increase in cells containing both a G1 and a G2 DNA content, suggesting that the cells treated with BN82002 are arrested at various stages of the cell cycle[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

359.42

Formula

C₁₉H₂₅N₃O₄

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 150 mg/mL (417.34 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7823 mL 13.9113 mL 27.8226 mL
5 mM 0.5565 mL 2.7823 mL 5.5645 mL
10 mM 0.2782 mL 1.3911 mL 2.7823 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.96 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.96 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Product Name:
BN82002
Cat. No.:
HY-112776
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