1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. Biocytin

Biocytin (Synonyms: (+)-Biocytin)

Cat. No.: HY-101884 Purity: 98.50%
Handling Instructions

Biocytin is a conjugate of D-biotin and L-lysine, where the carboxylate of D-biotin is coupled with the ϵ-amine of L-lysine via a secondary amide bond. Biocytin is a classical neuroanatomical tracer commonly used to map brain connectivity. Biocytin is used as a versatile marker in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays.

For research use only. We do not sell to patients.

Biocytin Chemical Structure

Biocytin Chemical Structure

CAS No. : 576-19-2

Size Price Stock Quantity
Solution
10 mM * 1 mL in Water USD 66 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 66 In-stock
Estimated Time of Arrival: December 31
Solid
10 mg USD 60 In-stock
Estimated Time of Arrival: December 31
25 mg USD 84 In-stock
Estimated Time of Arrival: December 31
50 mg USD 108 In-stock
Estimated Time of Arrival: December 31
100 mg USD 162 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

Biocytin is a conjugate of D-biotin and L-lysine, where the carboxylate of D-biotin is coupled with the ϵ-amine of L-lysine via a secondary amide bond. Biocytin is a classical neuroanatomical tracer commonly used to map brain connectivity. Biocytin is used as a versatile marker in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays[1][2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Due to its high affinity for avidin, Biocytin can be visualized by using a host of avidin-conjugated markers at the light and electron microscopic level. Biocytin is used to visualize the morphology of dendritic and axonal arborizations[1].
The Biocytin wide-field bipolar cell in rabbit retina is a sparsely populated ON cone bipolar cell with a broad dendritic arbor that does not contact all cones in its dendritic field. Identification of bipolar cells by selective uptake of Biocytin, labeled the cones with peanut agglutinin, and then identification of cone types using antibodies against blue cone opsin and red-green cone opsin. The Biocytin-labeled cells selectively contact cones whose outer segments stained for blue cone opsin and avoided cones that do not. Biocytin wide-field bipolar cell is an ON blue cone bipolar cell in the rabbit retina and is homologous to the blue cone bipolar cells that have been previously described in primate, mouse, and ground squirrel retinas[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

372.48

Formula

C16H28N4O4S

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 6 mg/mL (16.11 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6847 mL 13.4235 mL 26.8471 mL
5 mM 0.5369 mL 2.6847 mL 5.3694 mL
10 mM 0.2685 mL 1.3424 mL 2.6847 mL
*Please refer to the solubility information to select the appropriate solvent.
References

Purity: 98.50%

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Biocytin
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