Biocytin
Based on 6 publication(s) in Google Scholar
Biocytin is a conjugate of D-biotin and L-lysine, where the carboxylate of D-biotin is coupled with the -amine of L-lysine via a secondary amide bond. Biocytin is a classical neuroanatomical tracer commonly used to map brain connectivity. Biocytin is used as a versatile marker in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays.
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 576-19-2
- Formula: C16H28N4O4S
- Molecular Weight:372.48
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Publications Citing Use of MedChemExpress (MCE) Biocytin
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Biological Activity
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Human Endogenous Metabolite |
Due to its high affinity for avidin, Biocytin can be visualized by using a host of avidin-conjugated markers at the light and electron microscopic level. Biocytin is used to visualize the morphology of dendritic and axonal arborizations[1].
The Biocytin wide-field bipolar cell in rabbit retina is a sparsely populated ON cone bipolar cell with a broad dendritic arbor that does not contact all cones in its dendritic field. Identification of bipolar cells by selective uptake of Biocytin, labeled the cones with peanut agglutinin, and then identification of cone types using antibodies against blue cone opsin and red-green cone opsin. The Biocytin-labeled cells selectively contact cones whose outer segments stained for blue cone opsin and avoided cones that do not. Biocytin wide-field bipolar cell is an ON blue cone bipolar cell in the rabbit retina and is homologous to the blue cone bipolar cells that have been previously described in primate, mouse, and ground squirrel retinas[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 576-19-2
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Appearance Solid
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Molecular Weight 372.48
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Formula C16H28N4O4S
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Color White to off-white
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SMILES
N[C@@H](CCCCNC(CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O)=O)C(O)=O
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Synonyms
(+)-Biocytin
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (6)
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Journal Impact Factor
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Most Recent
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Acta Pharmacol Sin
Effects of Kv1.3 knockout on pyramidal neuron excitability and synaptic plasticity in piriform cortex of mice. [Abstract]2024 Oct;45(10):2045-2060. PMID: 38862816
Biocytin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 Oct;45(10):2045-2060. [Abstract]
Representative pyramidal neuron labeled with Biocytin (3 %) and its action potential in the second layer of the piriform cortex.
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ACS Environ Au
Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]2025 Aug 5;5(6):573-582. PMID: 41277996 -
Cell Rep
Green light relieves stress-induced anxiety-like behaviors via visual-to-prefrontal projections in mice. [Abstract]2026 May 23;45(6):117426. PMID: 42176269 -
Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
J Neurochem
Somatostatin interneurons inhibit excitatory transmission mediated by astrocytic GABAB and presynaptic GABAB and adenosine A1 receptors in the hippocampus. [Abstract]2022 Nov;163(4):310-326. PMID: 35775994
Biocytin purchased from MedChemExpress. Usage Cited in: J Neurochem. 2022 Nov;163(4):310-326. [Abstract]
The spreading of intracellular solution in the glial syncytium was visualized using Biocytin (0.2 %).
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Solvent & Solubility
H2O : 50 mg/mL (134.24 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (268.47 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Purity & Documentation
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Data Sheet (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Mishra A, et al. Improved neuronal tract tracing with stable biocytin-derived neuroimaging agents. ACS Chem Neurosci. 2010 Feb 17;1(2):129-38. [Content Brief]
[2]. Roth G, et al. Morphology and axonal projection pattern of neurons in the telencephalon of the fire-bellied toad Bombina orientalis: an anterograde, retrograde, and intracellular biocytin labeling study. J Comp Neurol. 2004 Oct 4;478(1):35-61. [Content Brief]
[3]. MacNeil MA, et al. Biocytin wide-field bipolar cells in rabbit retina selectively contact blue cones. J Comp Neurol. 2008 Jan 1;506(1):6-15. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.6847 mL | 13.4235 mL | 26.8471 mL | 67.1177 mL |
| 5 mM | 0.5369 mL | 2.6847 mL | 5.3694 mL | 13.4235 mL | |
| 10 mM | 0.2685 mL | 1.3424 mL | 2.6847 mL | 6.7118 mL | |
| 15 mM | 0.1790 mL | 0.8949 mL | 1.7898 mL | 4.4745 mL | |
| 20 mM | 0.1342 mL | 0.6712 mL | 1.3424 mL | 3.3559 mL | |
| 25 mM | 0.1074 mL | 0.5369 mL | 1.0739 mL | 2.6847 mL | |
| 30 mM | 0.0895 mL | 0.4475 mL | 0.8949 mL | 2.2373 mL | |
| 40 mM | 0.0671 mL | 0.3356 mL | 0.6712 mL | 1.6779 mL | |
| 50 mM | 0.0537 mL | 0.2685 mL | 0.5369 mL | 1.3424 mL | |
| 60 mM | 0.0447 mL | 0.2237 mL | 0.4475 mL | 1.1186 mL | |
| 80 mM | 0.0336 mL | 0.1678 mL | 0.3356 mL | 0.8390 mL | |
| 100 mM | 0.0268 mL | 0.1342 mL | 0.2685 mL | 0.6712 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Biocytin Related Classifications
HY-101884 Related Classifications
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