1. Stem Cell/Wnt
  2. β-catenin
    Wnt
  3. Cardiogenol C hydrochloride

Cardiogenol C hydrochloride 

Cat. No.: HY-12319A Purity: 96.22%
Handling Instructions

Cardiogenol C hydrochloride is a potent cell-permeable pyrimidine inducer which prompts the differentiation of ESCs into cardiomyocytes (EC50=100 nM). Cardiogenol C hydrochloride also acts cardiomyogenic on already lineage-committed progenitor cell types with a limited degree of plasticity. Cardiogenol C hydrochloride is a useful cardiomyogenic agent and can be used as a tool to improve cardiac repair by cell transplantation therapy in animal models.

For research use only. We do not sell to patients.

Cardiogenol C hydrochloride Chemical Structure

Cardiogenol C hydrochloride Chemical Structure

CAS No. : 1049741-55-0

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10 mM * 1 mL in DMSO USD 66 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Cardiogenol C hydrochloride is a potent cell-permeable pyrimidine inducer which prompts the differentiation of ESCs into cardiomyocytes (EC50=100 nM)[1]. Cardiogenol C hydrochloride also acts cardiomyogenic on already lineage-committed progenitor cell types with a limited degree of plasticity. Cardiogenol C hydrochloride is a useful cardiomyogenic agent and can be used as a tool to improve cardiac repair by cell transplantation therapy in animal models[2].

IC50 & Target

EC50: 100 nM (differentiation of ESCs into cardiomyocytes)[1]

In Vitro

Cardiogenol C hydrochloride (1 μM; 7 days) has a cardiomyogenic effect on P19 cells, it significantly increases atrial natriuretic factor (ANF, nppa) in P19 cells when it compares to untreated control cells[1].
Cardiogenol C hydrochloride (0.01-100 μM; 7 days) significantly increases ANF expression. In addition, another frequently used cardiac marker gene (NKX2-5) is also significantly increased by this small molecule in C2C12 cells[2].
Cardiogenol C hydrochloride (0.001-100 μM; 7 days) increases cardiac Nav1.5 sodium channel protein expression as dose-dependent manner in C2C12 cells[2].
Cardiogenol C hydrochloride (1 μM; 35 days) addition from day 0 significantly increases myocardial differentiation and results in a significantly increased percentage of CBs with beating cardiomyocytes. This small moleculepromotes the development of beating cardiomyocytes in cardiovascular progenitor cell-derived cardiac bodies[2].
Cardiogenol C hydrochloride (0.01-100 μM; 7 days) does not effect cell growth even at 10 μM. In addition, Cardiogenol C either solves in water or DMSO generates a similar effect. The highest concentration, 100 μM has significant cellular toxicity on C2C12 cells[2].

RT-PCR[2]

Cell Line: C2C12 cells
Concentration: 0.01 μM; 0.1 μM; 1 μM; 3 μM; 10 μM; 30 μM; 100 μM
Incubation Time: 7 days
Result: Increased ANF and NKX2.5 mRNA level as a dose-dependent manner.

Western Blot Analysis[2]

Cell Line: C2C12 cells
Concentration: 0.001 μM; 0.01 μM; 0.1 μM; 1 μM; 10 μM
Incubation Time: 7 days
Result: Increased cardiac Nav1.5 sodium channel protein levels.

Cell Proliferation Assay[2]

Cell Line: C2C12 cells
Concentration: 0.01 μM; 0.1 μM; 1 μM
Incubation Time: 7 days
Result: Did not exert toxic effects on C2C12 cells at 0.01-10 μM treatment.
Molecular Weight

296.75

Formula

C₁₃H₁₇ClN₄O₂

CAS No.

1049741-55-0

SMILES

[H]Cl.COC1=CC=C(NC2=NC=CC(NCCO)=N2)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 59 mg/mL (198.82 mM)

H2O : 2 mg/mL (6.74 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3698 mL 16.8492 mL 33.6984 mL
5 mM 0.6740 mL 3.3698 mL 6.7397 mL
10 mM 0.3370 mL 1.6849 mL 3.3698 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (8.42 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (8.42 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.42 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

Cardiogenol Cβ-cateninWntBeta cateninCardiomyogenicembryonicstemcellstransplantation,?progenitorplasticityescpyrimidineMHCInhibitorinhibitorinhibit

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Cardiogenol C hydrochloride
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