Cardiogenol C hydrochloride
Based on 1 Customer Validation
Cardiogenol C hydrochloride is a potent cell-permeable pyrimidine inducer which prompts the differentiation of ESCs into cardiomyocytes (EC50=100 nM). Cardiogenol C hydrochloride also acts cardiomyogenic on already lineage-committed progenitor cell types with a limited degree of plasticity. Cardiogenol C hydrochloride is a useful cardiomyogenic agent and can be used as a tool to improve cardiac repair by cell transplantation therapy in animal models.
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- Reinheit: 99.79%
- CAS. Nr.: 1049741-55-0
- Formel: C13H17ClN4O2
- Molecular Weight:296.75
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Speicherung:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
EC50: 100 nM (differentiation of ESCs into cardiomyocytes)[1]
Cardiogenol C hydrochloride (1 μM; 7 days) has a cardiomyogenic effect on P19 cells, it significantly increases atrial natriuretic factor (ANF, nppa) in P19 cells when it compares to untreated control cells[1].Cardiogenol C hydrochloride (0.01-100 μM; 7 days) significantly increases ANF expression. In addition, another frequently used cardiac marker gene (NKX2-5) is also significantly increased by this small molecule in C2C12 cells[2].Cardiogenol C hydrochloride (0.001-100 μM; 7 days) increases cardiac Nav1.5 sodium channel protein expression as dose-dependent manner in C2C12 cells[2].Cardiogenol C hydrochloride (1 μM; 35 days) addition from day 0 significantly increases myocardial differentiation and results in a significantly increased percentage of CBs with beating cardiomyocytes. This small moleculepromotes the development of beating cardiomyocytes in cardiovascular progenitor cell-derived cardiac bodies[2].Cardiogenol C hydrochloride (0.01-100 μM; 7 days) does not effect cell growth even at 10 μM. In addition, Cardiogenol C either solves in water or DMSO generates a similar effect. The highest concentration, 100 μM has significant cellular toxicity on C2C12 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:C2C12 cells
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Concentration:0.01 μM; 0.1 μM; 1 μM; 3 μM; 10 μM; 30 μM; 100 μM
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Incubation Time:7 days
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Result:Increased ANF and NKX2.5 mRNA level as a dose-dependent manner.
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Cell Line:C2C12 cells
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Concentration:0.001 μM; 0.01 μM; 0.1 μM; 1 μM; 10 μM
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Incubation Time:7 days
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Result:Increased cardiac Nav1.5 sodium channel protein levels.
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Cell Line:C2C12 cells
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Concentration:0.01 μM; 0.1 μM; 1 μM
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Incubation Time:7 days
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Result:Did not exert toxic effects on C2C12 cells at 0.01-10 μM treatment.
Chemical Information
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CAS. Nr. 1049741-55-0
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Appearance Solid
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Molecular Weight 296.75
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Formel C13H17ClN4O2
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Color White to off-white
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SMILES
[H]Cl.COC1=CC=C(NC2=NC=CC(NCCO)=N2)C=C1
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Lösungsmittel & Löslichkeit
DMSO : ≥ 59 mg/mL (198.82 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 2 mg/mL (6.74 mM; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (8.42 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.42 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 3.33 mg/mL (11.22 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (274 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Wu X, et al. Small molecules that induce cardiomyogenesis in embryonic stem cells.J Am Chem Soc. 2004 Feb 18;126(6):1590-1. [Content Brief]
[2]. Mike AK, et al. Small molecule cardiogenol C upregulates cardiac markers and induces cardiac functional properties in lineage-committed progenitor cells. Cell Physiol Biochem. 2014;33(1):205-21. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.3698 mL | 16.8492 mL | 33.6984 mL | 84.2460 mL |
| 5 mM | 0.6740 mL | 3.3698 mL | 6.7397 mL | 16.8492 mL | |
| DMSO | 10 mM | 0.3370 mL | 1.6849 mL | 3.3698 mL | 8.4246 mL |
| 15 mM | 0.2247 mL | 1.1233 mL | 2.2466 mL | 5.6164 mL | |
| 20 mM | 0.1685 mL | 0.8425 mL | 1.6849 mL | 4.2123 mL | |
| 25 mM | 0.1348 mL | 0.6740 mL | 1.3479 mL | 3.3698 mL | |
| 30 mM | 0.1123 mL | 0.5616 mL | 1.1233 mL | 2.8082 mL | |
| 40 mM | 0.0842 mL | 0.4212 mL | 0.8425 mL | 2.1061 mL | |
| 50 mM | 0.0674 mL | 0.3370 mL | 0.6740 mL | 1.6849 mL | |
| 60 mM | 0.0562 mL | 0.2808 mL | 0.5616 mL | 1.4041 mL | |
| 80 mM | 0.0421 mL | 0.2106 mL | 0.4212 mL | 1.0531 mL | |
| 100 mM | 0.0337 mL | 0.1685 mL | 0.3370 mL | 0.8425 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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