Crosstide
Based on 2 publication(s) in Google Scholar
Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt.
For research use only. We do not sell to patients.
- Purity: 98.37%
- CAS No.: 171783-05-4
- Formula: C48H77N17O17
- Molecular Weight:1164.23
-
Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocol
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Crosstide
More
Biological Activity
16HBE14o? cells exposed to RV39 demonstrate a crosstide kinase activity in vitro. Serine phosphorylation of crosstide is confirmed by immunoblotting, and phosphorylation is blocked by PP2 but not by PP3[1]. The wildtype GST-AKT2 shows significant phosphoryl transferase activity towards crosstide, reaching an initial velocity of 16 pmol phosphate/min/μg enzyme. The mutant GST-AKT2T/E,S/D displayed an initial velocity of 85 pmol phosphate/min/μg kinase for phosphorylation of Crosstide, corresponding to a 5-fold increase compared to the wildtype enzyme[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 171783-05-4
-
Appearance Solid
-
Molecular Weight 1164.23
-
Formula C48H77N17O17
-
Color White to off-white
-
Sequence
Gly-Arg-Pro-Arg-Thr-Ser-Ser-Phe-Ala-Glu-Gly
-
Sequence Shortening
GRPRTSSFAEG
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
-
Journal Impact Factor
-
Most Recent
-
FASEB J
2021 May;35(5):e21526. PMID: 33813773 -
Solvent & Solubility
H2O : ≥ 50 mg/mL (42.95 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
After serum deprivation for 24 h, cells are incubated with digoxigenin-labeled sham protein or digoxigenin-labeled RV39 at an MOI of 1.0 for 10 min. Cell homogenates are immunoprecipitated with mouse anti-digoxigenin antibody and precipitates incubated with Crosstide and [γ-32P]ATP. Crosstide is a glycogen synthase kinase α/β fusion protein sequence (GRPRTSSFAEG) which is a substrate for Akt. Samples are processed for autoradiography and immunoblotting using rabbit anti-phospho-Tyr416 Src, mouse anti-Src (clone GD11), rabbit anti-phospho-Ser473, or rabbit anti-Akt.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Bentley JK, et al. Rhinovirus activates interleukin-8 expression via a Src/p110beta phosphatidylinositol 3-kinase/Akt pathway in human airway epithelial cells. J Virol. 2007 Feb;81(3):1186-94. Epub 2006 Nov 22. [Content Brief]
[2]. Baer K, et al. Activation of a GST-tagged AKT2/PKBbeta. Biochim Biophys Acta. 2005 Oct 10;1725(3):340-7. Epub 2005 Apr 20. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.8589 mL | 4.2947 mL | 8.5894 mL | 21.4734 mL |
| 5 mM | 0.1718 mL | 0.8589 mL | 1.7179 mL | 4.2947 mL | |
| 10 mM | 0.0859 mL | 0.4295 mL | 0.8589 mL | 2.1473 mL | |
| 15 mM | 0.0573 mL | 0.2863 mL | 0.5726 mL | 1.4316 mL | |
| 20 mM | 0.0429 mL | 0.2147 mL | 0.4295 mL | 1.0737 mL | |
| 25 mM | 0.0344 mL | 0.1718 mL | 0.3436 mL | 0.8589 mL | |
| 30 mM | 0.0286 mL | 0.1432 mL | 0.2863 mL | 0.7158 mL | |
| 40 mM | 0.0215 mL | 0.1074 mL | 0.2147 mL | 0.5368 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Crosstide Related Classifications
HY-P0315 Related Classifications
Powered by Bioz