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  3. Crosstide

Crosstide 

Cat. No.: HY-P0315 Purity: 95.70%
Handling Instructions

Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Crosstide Chemical Structure

Crosstide Chemical Structure

CAS No. : 171783-05-4

Size Price Stock Quantity
1 mg USD 132 In-stock
Estimated Time of Arrival: December 31
5 mg USD 528 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt.

IC50 & Target[2]

Akt

 

In Vitro

16HBE14o− cells exposed to RV39 demonstrate a crosstide kinase activity in vitro. Serine phosphorylation of crosstide is confirmed by immunoblotting, and phosphorylation is blocked by PP2 but not by PP3[1]. The wildtype GST-AKT2 shows significant phosphoryl transferase activity towards crosstide, reaching an initial velocity of 16 pmol phosphate/min/μg enzyme. The mutant GST-AKT2T/E,S/D displayed an initial velocity of 85 pmol phosphate/min/μg kinase for phosphorylation of Crosstide, corresponding to a 5-fold increase compared to the wildtype enzyme[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1164.23

Formula

C₄₈H₇₇N₁₇O₁₇

CAS No.
Sequence

Gly-Arg-Pro-Arg-Thr-Ser-Ser-Phe-Ala-Glu-Gly

Sequence Shortening

GRPRTSSFAEG

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -80°C 2 years
-20°C 1 year
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : ≥ 50 mg/mL (42.95 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8589 mL 4.2947 mL 8.5894 mL
5 mM 0.1718 mL 0.8589 mL 1.7179 mL
10 mM 0.0859 mL 0.4295 mL 0.8589 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[1]

After serum deprivation for 24 h, cells are incubated with digoxigenin-labeled sham protein or digoxigenin-labeled RV39 at an MOI of 1.0 for 10 min. Cell homogenates are immunoprecipitated with mouse anti-digoxigenin antibody and precipitates incubated with Crosstide and [γ-32P]ATP. Crosstide is a glycogen synthase kinase α/β fusion protein sequence (GRPRTSSFAEG) which is a substrate for Akt. Samples are processed for autoradiography and immunoblotting using rabbit anti-phospho-Tyr416 Src, mouse anti-Src (clone GD11), rabbit anti-phospho-Ser473, or rabbit anti-Akt.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

CrosstideAktPKBProtein kinase BInhibitorinhibitorinhibit

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Crosstide
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HY-P0315
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